IRS1 antibody

Principal name

IRS1 antibody

Alternative names for IRS1 antibody

Insulin receptor substrate 1, IRS-1

SwissProt ID

P35568 (Human), P35569 (Mouse), P35570 (Rat)

Gene ID

3667 (IRS1), 16367 (Irs1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Mky (Monkey), Por (Porcine)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Enzyme Immunoassay (E), Dot blot (Dot), Immunoprecipitation (IP), ELISA (detection) (E(detection))

Background of IRS1 antibody

The insulin receptor substrate-1 (IRS-1), a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor. The association of 14-3-3 with IRS-1 increases significantly upon treatment with okadaic acid, a potent serine/ threonine phosphatase inhibitor. Therefore, the association of 14-3-3 protein may play a role in the regulation of insulin sensitivity by interrupting the association between the insulin receptor and IRS-1.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

IRS1 pSer616 antibody

Indirect ELISA: Serial dilution of the antibody starting with 5 µg/well was done to check for the affinity. 1 µg of coated antigen can be sensitively detected by anti-Human IRS pSer616 antibody used at 300 pg/well concentration. Mouse IgG1 HIR-B1 Aff - Purified Hu E, WB
0.1 mg / €400.00
  OriGene Technologies GmbH

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