ISCU / NIFUN antibody

Principal name

ISCU / NIFUN antibody

Alternative names for ISCU / NIFUN antibody

NifU-like N-terminal domain-containing protein, NifU-like protein, Iron-sulfur cluster assembly enzyme ISCU mitochondrial

SwissProt ID

Q9D7P6, Q9H1K1

Gene ID

66383 (Iscu), 23479 (ISCU)

Available reactivities

Bov (Bovine), Can (Canine), GP (Guinea Pig), Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of ISCU / NIFUN antibody

Iron-sulfur cluster assembly enzyme, also known as ISCU, is a member of the nifU family. Iron-sulfur (Fe-S) clusters are necessary for several mitochondrial enzymes and other subcellular compartment proteins. It is interact with ISCS, a cysteine desulfurase, to sequester inorganic sulfur for Fe-S cluster assembly. ISCU-ISCS protein complex localizes in both mitochondria and cytosol, implying that Fe-S cluster assembly takes place in multiple subcellular compartments in mammalian cells. Recombinant human ISCU protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

General readings

Tong WH., et al. (2000) EMBO J. 19:5692-5700.
Tong WH, et al. (2006). Cell Metab. 3 (3): 199–210.

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  2. Highly Validated (1)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ISCU / NIFUN antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ISCU mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA803397) Mouse IgG1 OTI4F5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ISCU / NIFUN antibody

WB Suggested Anti-Iscu Antibody; Titration: 1.0 ug/ml; Positive Control: Rat Lung Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

ISCU / NIFUN antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ISCU (RC212031, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ISCU. Mouse IgG1 OTI8H2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ISCU / NIFUN antibody

ISCU / NIFUN Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

ISCU / NIFUN antibody

Immunofluorescence of monoclonal antibody to NIFUN on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG1 3B8-1C4 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ISCU / NIFUN antibody

ISCU / NIFUN Mouse IgG1 3D12-1D10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ISCU / NIFUN (transcript variant 1)

ISCU / NIFUN Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

ISCU / NIFUN (35-167, His-tag)

ISCU / NIFUN Human Purified > 90 % E. coli
50 µg / €750.00
  Acris Antibodies GmbH

ISCU / NIFUN (35-167, His-tag)

ISCU / NIFUN Human Purified > 90 % E. coli
10 µg / €300.00
  Acris Antibodies GmbH

ISCU / NIFUN

ISCU / NIFUN Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

ISCU / NIFUN

ISCU / NIFUN Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

ISCU / NIFUN

ISCU / NIFUN Human Purified
10 µg / €380.00
  Novus Biologicals Inc.

ISCU / NIFUN

ISCU / NIFUN Human Purified
50 µg / €870.00
  Novus Biologicals Inc.

Lysates

Catalog No.    

NIFUN 293T Cell Transient Overexpression Lysate(Denatured)

NIFUN 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

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