ISG12a / IFI27 antibody

Principal name

ISG12a / IFI27 antibody

Alternative names for ISG12a / IFI27 antibody

p27, IFI-27, Interferon alpha-inducible protein 27,Interferon-alpha-induced 11.5 kDa protein, Interferon-stimulated gene 12a protein

SwissProt ID

P40305 (Human)

Gene ID

3429 (IFI27)

Available reactivities

Hu (Human)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Dot blot (Dot), Paraffin Sections (P)

Background of ISG12a / IFI27 antibody

Treatment of breast carcinoma MCF7cells with estradiol led to induction of a new 27 kDa protein, the cDNA was later cloned and identified as p27. This highly hydrophobic protein of 122 amino acids has 33% over all sequence similarity to the product of the 6-16 gene (1), that is induced by alfa/beta type interferons. It has been shown that p27is transcriptionaly induced by interferon alfa in various human cell lines. The induction induced by interferon alfa is independent of the presence of estradiol receptors on the cells. High levels of p27 mRNA, detected by Northern blotting, was found in 50% of the primary breast carcinoma cells, suggesting p27 can be a diagnostic marker for breast carcinoma detection (2). Examination of p27 expression by in situ hybridization in p27 over expressing tumors suggest that p27 gene is localized in cancer cells and some times also in fibroblastic cells of tumor stroma. IFI27 mRNA expression is highly up-regulated in lesional psoriatic epidermis and in non-lesional keratinocytes. It was also expressed in lichen planus, chronic eczema, cutaneous squamous cell cancers, and during normal wound repair when IFI27 was found in the proliferating subpopulation of keratinocytes (3). P27 Further studies are now necessary to elucidate the cause of p27 gene overexpression in breast carcinoma and in particular to determine whether it corresponds to chromosomal rearrangements in the 14q32 region and/or to induction by interferons of the alpha/beta type.
P27 protein is highly hydrophobic, containing 2 trans-membrane spanning regions localized on human chromosome in band q32. The apparent molecular weight of IFI27 is 26-29 kDa on reduced SDS-PAGE. IFI induced proteins like P27 and P16 genes have also showed potential targets for anti-angiogenic therapy employing INFs.

General readings

1. R. L. Friedman, S. P. Manly, M. McMahon, I. M. Kerr, and G. R. Stark, Cell, 38: 745-755, 1984.
2. Rasmussen UB, Wolf C, Mattei MG, Chenard MP, Bellocq JP, Chambon P, Rio MC, Basset P. Identification of a new interferonalphainducible
gene (p27) on human chromosome 14q32 and its expression in breast carcinoma. Cancer Res. 1993 Sep 1;53(17):4096-101.
3. Suomela S, Cao L, Bowcock A, Saarialho-Kere U. Interferon alpha-inducible protein 27 (IFI27) is upregulated in psoriatic skin and certain epithelial cancers. J Invest Dermatol. 2004 Mar;122(3):717-21.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ISG12a / IFI27 control peptide

ISG12a / IFI27
0.25 mg / €250.00
  Acris Antibodies GmbH

ISG12a / IFI27 (N-term) antibody

ISG12a / IFI27 Rabbit Aff - Purified Hu Dot, E
0.1 mg / €420.00
  Acris Antibodies GmbH

ISG12a / IFI27 control peptide

ISG12a / IFI27
0.25 mg / €250.00
  Acris Antibodies GmbH

ISG12a / IFI27 (C-term) antibody

ISG12a / IFI27 Rabbit Aff - Purified Hu Dot, E
0.1 mg / €420.00
  Acris Antibodies GmbH

ISG12a / IFI27 antibody

ISG12a / IFI27 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

ISG12a / IFI27 antibody

Western Blot analysis of IFI27 expression in transfected 293T cell line (H00003429-T02) by IFI27 MaxPab polyclonal antibody.

Lane 1: IFI27 transfected lysate(11.30 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

ISG12a / IFI27 antibody

ISG12a / IFI27 Mouse IgG1 4B8-G2 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

ISG12a / IFI27 antibody

ISG12a / IFI27 Rabbit Hu Dot, E
0.1 mg / €470.00
  Abnova Taiwan Corp.

ISG12a / IFI27 antibody

ISG12a / IFI27 Rabbit Hu Dot, E
0.1 mg / €470.00
  Abnova Taiwan Corp.

ISG12a / IFI27 antibody

ISG12a / IFI27 Rabbit Aff - Purified Hu E
0.1 mg / €440.00
  Novus Biologicals Inc.

ISG12a / IFI27 antibody

Immunohistochemistry-Paraffin: IFI27 Antibody [NBP1-84745] Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ISG12a / IFI27

ISG12a / IFI27 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

ISG12a / IFI27

ISG12a / IFI27 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates (Overexpressed)

Catalog No.    

IFI27 overexpression lysate

IFI27 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

14 Item(s)

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