ISG15 / G1P2 antibody

Principal name

ISG15 / G1P2 antibody

Alternative names for ISG15 / G1P2 antibody

UCRP, Interferon-induced 15 kDa protein, Interferon-induced 17 kDa protein, Ubiquitin cross-reactive protein

SwissProt ID

O02741 (Bovin), P05161 (Human), Q64339 (Mouse)

Gene ID

9636 (ISG15)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), Gt (Goat), Por (Porcine), Rb (Rabbit), Sh (Sheep)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection))

Background of ISG15 / G1P2 antibody

Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. ISG15 (Interferon Stimulating Gene-15) shows no amino acid sequence homology to cytokines and is synthesized as a precursor that is activated through processing by a thiol protease. ISG15 is secreted by
monocytes and lymphocytes. Synthesis is induced in response to IFN-α or IFN-β or IFN-∞, but not IFN-γ. ISG15 expression is induced also by overexpression of some interferon regulatory factors that have been shown to play a role in the transcriptional regulation of IFN genes. ISG15 is secreted also by cell lines of monocyte (U937 cell line), T-lymphocyte,
B-lymphocyte (DAUDI cells), human fibroblasts, and epithelial origins. The induction of terminal differentiation in human melanoma cells is associated, among other things, with alterations in the expression of ISG15. Intracellularly ISG15 has been shown to function as a ubiquitin homologue. It is known also as UCRP (ubiquitin cross-reactive protein). Serpin 2a (spi2a), a member of the serine protease inhibitor (serpin) protein family that is highly induced in macrophages during bacillus Calmette-Guerin infection has been shown to bind ISG15. ISG15 has been shown to modulate immune cell function. It possesses activities of cytokines and induces production of IFN-γ. It enhances proliferation and functions of natural killer and LAK cells.

General readings

D'Cunha, J. et al. (1996) Immunoregulatory properties of ISG15 interferon-induced cytokine. Proc. Natl, Acad. Sci. USA 93; 211-215.
Potter J.L.et al (1999) Precursor Processing of Pro-ISG15/UCRP, an Interferon--induced Ubiquitin-like Protein. J Biol Chem 274;35, 25061-25068.
Malakhov MP et al (2002) UBP43 (USP18) specifically removes ISG15 from conjugated proteins. J Biol Chem.277(12):9976-81.
Muller, S. , Hoege, C. , Pyrowolakis, G. and Jentsch, S. (2001) SUMO, ubiquitin's mysterious cousin. Nat Rev Mol Cell Biol, 2(3): 202-10.
Liakopoulos D et al. (1998). A novel protein modification pathway related to the ubiquitin system. EMBO J. 15;17(8):2208-14.
Jentsch S, Pyrowolakis G. (2000) Ubiquitin and its kin: how close are the family ties? Trends Cell Biol. 10(8):335-42.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ISG15 / G1P2 antibody

WB Suggested Anti-ISG15 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human kidney Rabbit IgG Purified Bov, Can, Eq, Gt, Hu, Por, Rb, Sh P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

ISG15 / G1P2 (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ISG15 / G1P2 antibody

ISG15 MaxPab rabbit polyclonal antibody. Western Blot analysis of ISG15 expression in mouse kidney. Rabbit Serum Hu, Ms E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

ISG15 / G1P2 antibody

ISG15 MaxPab rabbit polyclonal antibody. Western Blot analysis of ISG15 expression in mouse kidney. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

ISG15 / G1P2 (Center) antibody

Mouse Brain; Researcher: Dr. Yuzhi Chen, University of Arkansas for Medical Science. Application: IHC. Species+tissue/cell type: Mouse brain stem cells. Primary antibody dilution: 1:500. Secondary antibody: Goat anti-rabbit Alexa-Fluor 594. Secondary antibody dilution: 1:1000; ISG15 antibody - middle region (ARP59386_P050) in Mouse Brain cells using Immunohistochemistry Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

ISG15 / G1P2 antibody

Western blot analysis of ISG15(arrow) using rabbit polyclonal ISG15 Antibody (Center R87) (Cat.#TA302035). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with ISG15 gene (Lane 2) (Origene Technologies). Rabbit Ig Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ISG15 / G1P2 antibody

Predicted band size: 18 kDa . Positive control: Human brain tissue, Hela cell and mouse kidney tissue lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lane1: Human brain tissue lysate Lane2: Hela cell lysate Lane3: Mouse kidney tissue lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ISG15 / G1P2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ISG15 (RC201235, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ISG15.(1:2000) Mouse IgG1 OTI6C8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ISG15 / G1P2 (1-157) antibody

Western blot analysis: Cell lysates of HeLa (each 50 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ISG15 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 3E5 Purified Hu E, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

ISG15 / G1P2 (1-157) antibody

Western blot analysis: Cell lysates of HeLa (each 50 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ISG15 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 3E5 Purified Hu E, ICC/IF, WB
50 µl / €230.00
  Acris Antibodies GmbH

ISG15 / G1P2 antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Brain, cortex. Rabbit Purified Hu E, IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

ISG15 / G1P2 (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P
0.4 ml / €370.00
  Acris Antibodies GmbH

ISG15 / G1P2 antibody

Immunoblot of hISG15 fusion protein.
Anti-hISG15 antibody, generated by immunization
with recombinant human ISG15, was tested by immunoblot against a hISG15-GFP fusion protein produced in E.coli cell lysate soluble fraction.
Dilution of the antibody between 1:200 and 1:1,000
showed strong reactivity specifically with hISG15
and ISG15 coupled proteins. Free hISG15 is indicated by the arrowhead. In this blot the antibody was used at a 1:200 dilution incubated
overnight at 4° C in 5% non-fat dry milk in TTBS.
Detection occurred using a 1:2000 dilution of HRPlabeled
Donkey anti-Rabbit IgG for 1 hour at room temperature. A
chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results. Data contributed by M.Malakhov, personal Rabbit Purified Hu E, WB
0.5 mg / €440.00
  Acris Antibodies GmbH

ISG15 / G1P2 antibody

ISG15 / G1P2 Mouse Hu WB
50 µg / €370.00
  Abnova Taiwan Corp.

ISG15 / G1P2 antibody

Western blot analysis of HeLa cell lysate ( each 50 µg ) was resolved by SDS - PAGE , transferred to PVDF membrane and probed with ISG15 monoclonal antibody , clone 3E5 ( 1 : 1000 ) ( Cat # MAB1090 ) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 3E5 Hu E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

ISG15 / G1P2 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with ISG15 polyclonal antibody ( Cat # PAB1719 ) at a 1 : 50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu E, P
0.4 ml / €390.00
  Abnova Taiwan Corp.

ISG15 / G1P2 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with ISG15 polyclonal antibody ( Cat # PAB1720 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

ISG15 / G1P2 antibody

ISG15 / G1P2 Hu
0.5 mg / €390.00
  Abnova Taiwan Corp.

ISG15 / G1P2 antibody

Western Blot: ISG15 Antibody [NB600-891] - Immunoblot of hISG15 fusion protein. Anti-hISG15 antibody, generated by immunization with recombinant human ISG15, was tested by immunoblot against a hISG15-GFP fusion protein  produced in E.coli cell lysate soluble fraction. Dilution of the antibody between 1:200 and 1:1,000 showed strong reactivity specifically with hISG15 and ISG15 coupled proteins. Free hISG15 is  indicated by the arrowhead. In this blot the antibody was used at a 1:200 dilution incubated overnight at 4C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP labeled  Donkey anti-Rabbit IgG for 1h at room temperature. A chemiluminescence system was used for signal detection. Rabbit IgG Purified Hu E, P, WB
0.5 mg / €510.00
  Novus Biologicals Inc.

ISG15 / G1P2 antibody

ISG15 / G1P2 Mouse IgG1 3E5 Purified Hu E, WB
0.1 ml / €380.00
  Novus Biologicals Inc.

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