Isocitrate dehydrogenase / IDH antibody

Principal name

Isocitrate dehydrogenase / IDH antibody

Alternative names for Isocitrate dehydrogenase / IDH antibody

PICD, Cytosolic NADP-isocitrate dehydrogenase, ICDH, IDP, Oxalosuccinate decarboxylase, cytoplasmic Isocitrate dehydrogenase [NADP], NADP(+)-specific ICDH

SwissProt ID

O75874 (Human), O88844 (Mouse), P20304 (Pig), P41562 (Rat), P50217 (Soltu), Q6XUZ5 (Sheep), Q802Y2, Q9XSG3 (Bovin)

Gene ID

3417 (IDH1), 100006589 (idh1)

Available reactivities

Hu (Human), Ms (Mouse), Por (Porcine), Rb (Rabbit), Rt (Rat), African clawed frog, Bov (Bovine), Can (Canine), Sh (Sheep), Ze (Zebrafish), Mky (Monkey)

Available hosts

Rabbit, Goat, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Dot blot (Dot), Immunodiffusion (ID), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Radioimmunoassay (R), ELISA (detection) (E(detection)), Frozen Sections (C), Flow Cytometry (F), Luminex (LMNX)

Background of Isocitrate dehydrogenase / IDH antibody

Isocitrate dehydrogenase enzymes catalyze the oxidative decarboxylation of isocitrate to produce alfa ketoglutarate. The human genome has 5 IDH genes coding for 3 IDH enzymes. The IDH1 and IDH2 require nicotinamide adeninedinucleotide phosphate (NADP) as co-substrate, whereas IDH3 require nicotinamide adenine dinucleotide (NAD). The IDH 2 and 3 are localized in mitochondria and are actively involved in the citric acid cycle (TCA) for energy production in contrast, IDH1 is localized in cytoplasm and peroxisomes where it generates NADPH, reduced form of NADP for biosynthetic and other types of reaction (1). Since alfa KG and NADPH both are intermediately substrate for a number of cellular process, which allows the possibility of oncogenic or tumor suppressive activities of IDH1.
The high throughput DNA sequencing is providing an unprejudiced picture of cancer associated mutations across the genome. The identification of mutations in a specific isoform of IDH1 gene in glioblastoma multiforme suggest that this enzyme may be promoting oncogenesis (2). Heterozygous IDH1 mutations have been identified in up to 80% of certain types of glioblastoma multifome and remarkably all these mutations are confined to a single residue, conserved in all three IDH enzymes, Arginine 132 to histidine substitution. Additionally five more mutations have been identified (Ser, Cys, Gly, Val and Leu) which suggest that IDH1 may be a atypical tumor suppressor as a result of mutations causing a loss of function or an oncogne in which the mutation causes gain in function (1). The mutant IDH1 enzyme dimerizes with wild type IDH1 to form an inactive complex in hypoxia inducible factor mediated oncogenic pathway thus causing a decrease in alfa KG and dioxygenase family enzymes that catalyses the hydroxylation of HIF and make it susceptible to degradation (3). Other Arginine residues Arg100 and 109 of human IHD1 are implicated in isocitrate binding and mutations at these sites resulted in inactive enzyme (4). The mutations at Arg132 causes impairments in the affinity of IDH1 for its substrate and inhibit the wild type IDH1 activity by formation of catalytically inactive heterodimers (5). Thus IDH1appears to function as a tumor suppressor but mutational inactivated protein contribute tumorogenesis in part through induction of hypoxia inducible factor (5). IDH1 is a 414 amino acid protein (51kDa) located on chromosome 2q.33.3a and contains a PTS peroxisomal signal targeting sequence.

General readings

Thorsness P.E. et al. (1987) J. Biol. Chem. 262: 10422-10425.Geisbrecht B.V. et al. (1999) J. Biol. Chem. 274: 30527-30533.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Isocitrate dehydrogenase / IDH antibody

Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-IDH1  mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500610, Dilution 1:50) Mouse IgG1 OTI2H9 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Isocitrate dehydrogenase / IDH antibody

Western blot analysis of extracts of MCF7 cell lines, using IDH1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Isocitrate dehydrogenase / IDH antibody

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-IDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800406) Mouse IgG1 OTI3G9 Purified Can, Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Isocitrate dehydrogenase / IDH antibody

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-IDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800426) Mouse IgG2b OTI24A2 Purified Can, Hu, Mky, Ms, Rt P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Isocitrate dehydrogenase / IDH antibody

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-IDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800427) Mouse IgG1 OTI1D1 Purified Can, Hu, Mky, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Isocitrate dehydrogenase / IDH antibody

Human Testis; Rabbit Anti-IDH1 Antibody. Catalog Number: ARP54787. Paraffin Embedded Tissue: Human Testis. Antibody Concentration: 5 ug/ml; IDH1 antibody - C-terminal region (ARP54787_P050) in Human Testis cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Hu, Ms, Rb, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

Isocitrate dehydrogenase / IDH antibody

Human Testis; IDH1 antibody - C-terminal region (ARP54788_P050) in Human Testis cells using Immunohistochemistry Rabbit Aff - Purified Bov, Hu, Ms, Rb, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

Isocitrate dehydrogenase / IDH antibody

Immunohistochemistry-Paraffin: Isocitrate dehydrogenase Antibody (2H9) [NBP1-47804] - Immunohistochemical staining of paraffin-embedded breast tissue using anti-IDH1 mouse monoclonal antibody. (Dilution 1:50) Mouse IgG1 2H9 Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €530.00
  Novus Biologicals Inc.

Isocitrate dehydrogenase / IDH (153-164) antibody

AP22440PU-N IDH1 antibody staining of HepG2 lysate at 0.3 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rb, Rt E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Isocitrate dehydrogenase / IDH (65-77) antibody

AP22441PU-N IDH1 antibody staining of HepG2 lysate at 0.1 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt, Sh E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Isocitrate dehydrogenase / IDH antibody

HEK293T cells were transfected with pCMV6-ENTRY control (Lane 1) or pCMV6-ENTRY IDH1 (Lane 2) plasmid for 48 hrs. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDH1-biotin and Streptavidin-HRP. Mouse IgG1 OTI2H9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Isocitrate dehydrogenase / IDH antibody

HEK293T cells were transfected with pCMV6-ENTRY control (Lane 1) or pCMV6-ENTRY IDH1 (Lane 2) plasmids for 48 hrs. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDH1-HRP. Mouse IgG1 OTI2H9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Isocitrate dehydrogenase / IDH antibody

HEK293T cells were transfected with pCMV6-ENTRY control (PS01, left panel) or IDH1 cDNA (right panel) and incubated for 48 hours after transfection. The cells were detached, fixed with 2% formaldehyde for 30 min, permeabilized with 0.1% trixon-X100, and then stained with PE-labeled IDH1 antibodies (TA400028). Mouse IgG1 OTI2H9 Purified Hu F
0.1 ml / €325.00
  OriGene Technologies, Inc.

Isocitrate dehydrogenase / IDH antibody

IDH1 Elisa with 2H9 Capture (TA600198) and 24A2 Detection (TA700199) Antibodies. Substrate used: Recombinant Human IDH1 (TP310582) Mouse IgG1 OTI2H9 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

Isocitrate dehydrogenase / IDH antibody

IDH1 Elisa with 24A2 Capture (TA600199) and 2H9 Detection (TA700198) Antibodies. Substrate used: Recombinant Human IDH1 (TP310582) Mouse IgG2b OTI24A2 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

Isocitrate dehydrogenase / IDH antibody

IDH1 Elisa with 24A2 Capture (TA600199) and 2H9 Detection (TA700198) Antibodies. Substrate used: Recombinant Human IDH1 (TP310582) Mouse IgG1 OTI2H9 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

Isocitrate dehydrogenase / IDH antibody

IDH1 Elisa with 2H9 Capture (TA600198) and 24A2 Detection (TA700199) Antibodies. Substrate used: Recombinant Human IDH1 (TP310582) Mouse IgG2b OTI24A2 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

Isocitrate dehydrogenase / IDH (IDH1/IDH2) antibody

AP08590PU-N Isocitrate Dehydrogenase antibody staining of Formalin-Fixed Paraffin-Embedded Human Heart at 10 µg/ml followed by biotinylated Goat anti-Rabbit IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen. Rabbit IgG Aff - Purified Hu, Ms, Por E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Isocitrate dehydrogenase / IDH Control Peptide

Isocitrate dehydrogenase / IDH
0.25 mg / €250.00
  Acris Antibodies GmbH

Isocitrate dehydrogenase / IDH (109-127) antibody

Western blot of AP10105PU-N at 1:500. Rabbit Aff - Purified Ms, Rt E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

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