Isocitrate dehydrogenase / IDH2 antibody

Principal name

Isocitrate dehydrogenase / IDH2 antibody

Alternative names for Isocitrate dehydrogenase / IDH2 antibody

mitochondrial Oxalosuccinate decarboxylase, ICDH, IDP, ICD-M, mitochondrial Isocitrate dehydrogenase [NADP], NADP(+)-specific ICDH

SwissProt ID

P33198 (Pig), P48735 (Human), P54071 (Mouse), P56574 (Rat), Q04467 (Bovin)

Gene ID

3418 (IDH2), 269951 (Idh2), 361596

Available reactivities

Hu (Human), Bov (Bovine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Can (Canine), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Isocitrate dehydrogenase / IDH2 antibody

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. IDH2 is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex.Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Shin,S.W., (2008) Biochem. Biophys. Res. Commun. 366 (4), 1012-1018

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Isocitrate dehydrogenase / IDH2 antibody

Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 5F11 Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

Isocitrate dehydrogenase / IDH2 antibody

  Rabbit Polyclonal antibody to IDH2 (isocitrate dehydrogenase 2 (NADP+), mitochondrial)  
Sample (50 ug of whole cell lysate). A: Mouse brain. 10% SDS PAGE. TA308664 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Isocitrate dehydrogenase / IDH2 antibody

  Goat Anti-IDH2 Antibody  
(0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Isocitrate dehydrogenase / IDH2 (C-term) antibody

  Rabbit Polyclonal IDH1 Antibody  
Western blot analysis of IDH1 in HepG2 cell lysate with IDH1 antibody at (A) 1 and (B) 2 ug/mL. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Isocitrate dehydrogenase / IDH2 (N-term) antibody

  Rabbit Polyclonal IDH2 Antibody  
Western blot analysis of IDH2 in human heart tissue lysate with IDH2 antibody at (A) 1 and (B) 2 ug/mL. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Isocitrate dehydrogenase / IDH2 antibody

  Rabbit Polyclonal IDH2 Antibody  
Western Blot: IDH2 Antibody [TA336931] - Western blot analysis of Caki-1 cell lysate (A), human skeletal muscle tissue (B), mouse skeletal muscle tissue ©, and rat skeletal muscle tissue (D) using IDH2 antibody at a concentration of 2 ug/ml. Rabbit Purified Hu, Ms, Rt C, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Isocitrate dehydrogenase / IDH2 antibody

IDH2 polyclonal antibody (A01). Western Blot analysis of IDH2 expression in human ovary cancer. Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Isocitrate dehydrogenase / IDH2 antibody

Immunoprecipitation of IDH2 transfected lysate using anti-IDH2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with IDH2 monoclonal antibody (M01), clone 5F11 (H00003418-M01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

Isocitrate dehydrogenase / IDH2 antibody

Immunoperoxidase of monoclonal antibody to IDH2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml] Mouse IgG1 5F11 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Isocitrate dehydrogenase / IDH2 antibody

Isocitrate dehydrogenase / IDH2 Mouse IgG2b 2G10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Isocitrate dehydrogenase / IDH2 antibody

Rat kidney; IDH2 antibody - middle region (ARP48207_P050) validated by WB using Proximal kidney tubules purfied from cortex at 1:1000.; IDH2 antibody - middle region (ARP48207_P050) in Rat kidney cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

CRISPR

Catalog No. Species Donor Vector  

Idh2 - mouse gene knockout kit via CRISPR

Idh2 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Idh2 - mouse gene knockout kit via CRISPR

Idh2 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Idh2 - mouse gene knockout kit via CRISPR

Idh2 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Idh2 - mouse gene knockout kit via CRISPR

Idh2 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Isocitrate dehydrogenase / IDH2

  Recombinant protein of human isocitrate dehydrogenase 2 (NADP+), mitochondrial (IDH2), nuclear gene encoding mitochondrial protein  
Isocitrate dehydrogenase / IDH2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Isocitrate dehydrogenase / IDH2 (C-term MYC/DDK tag)

  Recombinant protein of human isocitrate dehydrogenase 2 (NADP+), mitochondrial (IDH2 mutant R172K), nuclear gene encoding mitochondrial protein , with C-terminal MYC/DDK tag, expressed in human cells, 20ug  
Isocitrate dehydrogenase / IDH2 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Isocitrate dehydrogenase / IDH2 (C-term MYC/DDK tag)

  Recombinant protein of human isocitrate dehydrogenase 2 (NADP+), mitochondrial (IDH2 mutant R172M), nuclear gene encoding mitochondrial protein , with C-terminal MYC/DDK tag, expressed in human cells, 20ug  
Isocitrate dehydrogenase / IDH2 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Isocitrate dehydrogenase / IDH2 (C-term MYC/DDK tag)

  Recombinant protein of human isocitrate dehydrogenase 2 (NADP+), mitochondrial (IDH2 mutant R172G), nuclear gene encoding mitochondrial protein , with C-terminal MYC/DDK tag, expressed in human cells, 20ug  
Isocitrate dehydrogenase / IDH2 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Isocitrate dehydrogenase / IDH2 (C-term DDK tag)

  Purified recombinant protein of mutant(R172G) of Homo sapiens isocitrate dehydrogenase 2 (NADP+), mitochondrial (IDH2),with C-terminal DDK tag,expressed in sf9 cells, 20 µg  
Isocitrate dehydrogenase / IDH2 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
SF9 cells
20 µg / €399.00
  OriGene Technologies, Inc.

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