ITCH / AIF4 antibody

Principal name

ITCH / AIF4 antibody

Alternative names for ITCH / AIF4 antibody

NAPP1, E3 ubiquitin-protein ligase Itchy homolog, Atrophin-1-interacting protein 4, NFE2-associated polypeptide 1

SwissProt ID

Q8C863 (Mouse), Q96J02 (Human)

Gene ID

83737 (ITCH), 16396 (Itch)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Goat, Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P)

Background of ITCH / AIF4 antibody

Kit Component:
- KN308466G1, Itch gRNA vector 1 in pCas-Guide vector
- KN308466G2, Itch gRNA vector 2 in pCas-Guide vector
- KN308466D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Di Marcotullio L, Ferretti E, Greco A, De Smaele E, Po A, Sico MA, Alimandi M, Giannini G, Maroder M, Screpanti I, Gulino A.
Numb is a suppressor of Hedgehog signalling and targets Gli1 for Itch-dependent ubiquitination.
Nat Cell Biol. 2006 Dec;8(12):1415-23. Epub 2006 Nov 19.
PMID: 17115028

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ITCH / AIF4 antibody

TA302519 (0.03µg/ml) staining of Human Brain (Frontal Cortex) lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ITCH / AIF4 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue using ITCH (Phospho-Tyr420) antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ITCH / AIF4 antibody

Western blot analysis of extracts from mouse brain cells, using ITCH (Ab-420) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ITCH / AIF4 antibody

Western blot analysis of ITCH in 3T3 cell lysate with ITCH antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ITCH / AIF4 antibody

WB Suggested Anti-ITCH Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: COLO205 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

ITCH / AIF4 pTyr420 antibody

Immunohistochemical analysis of paraffin-embedded human brain tissue using ITCH (Phospho-Tyr420) antibody AP55793PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms P
0.1 ml / €340.00
  Acris Antibodies GmbH

ITCH / AIF4 pTyr420 antibody

Immunohistochemical analysis of paraffin-embedded human brain tissue using ITCH (Phospho-Tyr420) antibody AP55793PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms P
50 µl / €270.00
  Acris Antibodies GmbH

ITCH / AIF4 antibody

Western Blot analysis of ITCH expression in transfected 293T cell line (H00083737-T01) by ITCH MaxPab polyclonal antibody.

Lane1:ITCH transfected lysate(94.82 KDa).
Lane2:Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

ITCH / AIF4 antibody

ITCH / AIF4 Mouse IgG2b 1B8 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

ITCH / AIF4 antibody

ITCH polyclonal antibody ( Cat # PAB7215 ) ( 0.03 µg/ml ) staining of human brain ( frontal cortex ) lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

ITCH / AIF4 pTyr420 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using ITCH (Phospho-Tyr420) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
50 µg / €205.00
  Assay Biotech

ITCH / AIF4 pTyr420 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using ITCH (Phospho-Tyr420) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
0.1 mg / €310.00
  Assay Biotech

ITCH / AIF4 antibody

Western blot analysis of extracts from mouse brain cells, using ITCH (Ab-420) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €205.00
  Assay Biotech

ITCH / AIF4 antibody

Western blot analysis of extracts from mouse brain cells, using ITCH (Ab-420) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €310.00
  Assay Biotech

ITCH / AIF4 antibody

Human COLO205; WB Suggested Anti-ITCH Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: COLO205 cell lysate; ITCH antibody - N-terminal region (ARP43347_P050) in Human COLO205 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ITCH / AIF4

ITCH / AIF4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

ITCH / AIF4

ITCH / AIF4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

ITCH 293T Cell Transient Overexpression Lysate(Denatured)

ITCH 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

ITCH overexpression lysate

ITCH overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Itch (GFP-tagged) - Mouse itchy, E3 ubiquitin protein ligase (cDNA clone MGC:67157 IMAGE:6409535), 200 uL, >10^7 TU/mL

Lenti ORF particles, Itch (GFP-tagged) - Mouse itchy, E3 ubiquitin protein ligase (cDNA clone MGC:67157 IMAGE:6409535), 200 uL, >10^7 TU/mL  
200 µl / €1,390.00
  OriGene Technologies, Inc.

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