ITCH / AIF4 antibody

Principal name

ITCH / AIF4 antibody

Alternative names for ITCH / AIF4 antibody

NAPP1, E3 ubiquitin-protein ligase Itchy homolog, Atrophin-1-interacting protein 4, NFE2-associated polypeptide 1

SwissProt ID

Q8C863 (Mouse), Q96J02 (Human)

Gene ID

83737 (ITCH)

Available reactivities

Hu (Human), Ms (Mouse), Can (Canine), Rt (Rat)

Available hosts

Goat, Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of ITCH / AIF4 antibody

Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. It can act as a transcriptional corepressor of p45/NFE2 and may participate in the regulation of immune responses by modifying Notch-mediated signaling. It is highly similar to the mouse Itch protein, which has been implicated in the regulation and differentiation of erythroid and lymphoid cells. [provided by RefSeq]. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Di Marcotullio L, Ferretti E, Greco A, De Smaele E, Po A, Sico MA, Alimandi M, Giannini G, Maroder M, Screpanti I, Gulino A.
Numb is a suppressor of Hedgehog signalling and targets Gli1 for Itch-dependent ubiquitination.
Nat Cell Biol. 2006 Dec;8(12):1415-23. Epub 2006 Nov 19.
PMID: 17115028

Items 1 to 20 of 23 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ITCH / AIF4 antibody

TA302519 (0.03µg/ml) staining of Human Brain (Frontal Cortex) lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ITCH / AIF4 antibody

Western blot analysis of extracts from mouse brain cells, using ITCH (Ab-420) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ITCH / AIF4 antibody

Western blot analysis of ITCH in 3T3 cell lysate with ITCH antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ITCH / AIF4 antibody

WB Suggested Anti-ITCH Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: COLO205 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

ITCH / AIF4 pTyr420 antibody

Immunohistochemical analysis of paraffin-embedded human brain tissue using ITCH (Phospho-Tyr420) antibody AP55793PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms P
0.1 ml / €340.00
  Acris Antibodies GmbH

ITCH / AIF4 pTyr420 antibody

Immunohistochemical analysis of paraffin-embedded human brain tissue using ITCH (Phospho-Tyr420) antibody AP55793PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms P
50 µl / €270.00
  Acris Antibodies GmbH

ITCH / AIF4 antibody

Western Blot analysis of ITCH expression in transfected 293T cell line (H00083737-T01) by ITCH MaxPab polyclonal antibody.

Lane1:ITCH transfected lysate(94.82 KDa).
Lane2:Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

ITCH / AIF4 antibody

ITCH / AIF4 Mouse IgG2b 1B8 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

ITCH / AIF4 antibody

ITCH polyclonal antibody ( Cat # PAB7215 ) ( 0.03 µg/ml ) staining of human brain ( frontal cortex ) lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

ITCH / AIF4 pTyr420 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using ITCH (Phospho-Tyr420) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
50 µg / €205.00
  Assay Biotech

ITCH / AIF4 pTyr420 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using ITCH (Phospho-Tyr420) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
0.1 mg / €310.00
  Assay Biotech

ITCH / AIF4 antibody

Western blot analysis of extracts from mouse brain cells, using ITCH (Ab-420) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €205.00
  Assay Biotech

ITCH / AIF4 antibody

Western blot analysis of extracts from mouse brain cells, using ITCH (Ab-420) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €310.00
  Assay Biotech

ITCH / AIF4 antibody

Human COLO205; WB Suggested Anti-ITCH Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: COLO205 cell lysate; ITCH antibody - N-terminal region (ARP43347_P050) in Human COLO205 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

ITCH / AIF4 antibody

NB100-68142 (0.03ug/ml) staining of Human Brain (Frontal Cortex) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

ITCH / AIF4 antibody

Western Blot: Itch Antibody [NBP1-18881]-  Whole cell lysate (5, 15 and 50 mcg) from Jurkat cells. Affinity purified rabbit anti-Itch antibody used for WB at 0.04 mcg/ml. Rabbit IgG Aff - Purified Hu WB
0.1 ml / €440.00
  Novus Biologicals Inc.

ITCH / AIF4 antibody

ITCH / AIF4 Rabbit IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

ITCH / AIF4 antibody

ITCH / AIF4 Rabbit IgG Aff - Purified Hu, Ms E, WB
50 µg / €290.00
  Novus Biologicals Inc.

ITCH / AIF4 antibody

Immunohistochemistry-Paraffin: ITCH Antibody [NBP1-89180] - Immunohistochemical staining of human duodenum shows strong positivity in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ITCH / AIF4

ITCH / AIF4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Items 1 to 20 of 23 total

per page
Page:
  1. 1
  2. 2
  • LinkedIn