IZUMO1 antibody
Principal name
IZUMO1 antibody
Alternative names for IZUMO1 antibody
Izumo sperm-egg fusion protein 1
Gene ID
284359 (IZUMO1)
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of IZUMO1 antibody
IZUMO1, a sperm-specific protein, is essential for sperm-egg plasma membrane binding and fusion. It is involved in the fertilization process and in the fusion of the sperm with the egg (1,2). IZUMO1 is expressed as at least four isoforms produced by altertive splicing and contains one Ig-like (immunoglobulin-like) C2-type domain (3). Lack of IZUMO1 in males, due to homozygous mutation in the gene that encodes IZUMO1, results in sterility due to an ibility to penetrate the plasma membrane of the egg (4).
General readings
Saha,P., (er) Fertil. Steril. (2007) In press
Western Blot: IZUMO1 Lysate [NBL1-12090] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for IZUMO1
Human Heart; WB Suggested Anti-IZUMO1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human heart; IZUMO1 antibody - C-terminal region (ARP51341_P050) in Human Heart cells using Western Blot
Western Blot: IZUMO1 Antibody [NBP1-69692] - This Anti-IZUMO1 antibody was used in Western Blot of Fetal Heart tissue lysate at a concentration of 1ug/ml.
Immunohistochemistry-Paraffin: IZUMO1 Antibody [NBP1-83086] - Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.
Western Blot: IZUMO1 Antibody [NBP1-83086] Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: Negative control (vector only transfected HEK293T lysate) | Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
Detection of IZUMO1 in Fetal Heart using anti-IZUMO1 antibody (GTX45858) at 1 µg/mL.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA339199 | IZUMO1 antibody |
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 |
Rabbit | IgG | Purified | Hu | WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA349189 | IZUMO1 antibody |
||||||
 |
Rabbit | IgG | Purified | Hu | WB |
0.1 mg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| H00284359-B01P | IZUMO1 antibody |
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 |
Mouse | Hu | WB |
0.5 mg /
€1,400.00
|
|||
| Abnova Taiwan Corp. | |||||||
| ARP51341_P050 | IZUMO1 antibody |
||||||
 |
Rabbit | Aff - Purified | Hu | WB |
50 µg /
€350.00
|
||
| AVIVA Systems Biology | |||||||
| NBP1-69692 | IZUMO1 antibody |
||||||
![Western Blot: IZUMO1 Antibody [NBP1-69692] - This Anti-IZUMO1 antibody was used in Western Blot of Fetal Heart tissue lysate at a concentration of 1ug/ml.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/IZUMO1 Antibody-Western Blot-NBP1-69692-img0001.jpg) |
Rabbit | Aff - Purified | Hu | WB |
50 µg /
€440.00
|
||
| Novus Biologicals Inc. | |||||||
| NBP1-83086 | IZUMO1 antibody |
||||||
![Immunohistochemistry-Paraffin: IZUMO1 Antibody [NBP1-83086] - Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/IZUMO1 Antibody-Immunohistochemistry-Paraffin-NBP1-83086-0005.jpg) |
Rabbit | IgG | Aff - Purified | Hu | P, WB |
0.1 ml /
€500.00
|
|
| Novus Biologicals Inc. | |||||||
 +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP306671 | IZUMO1 |
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 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€680.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates (Overexpressed)
| Catalog No. | ||
|---|---|---|
| LY405465 | IZUMO1 overexpression lysate |
|
|
0.1 mg /
€280.00
|
|
| OriGene Technologies, Inc. |
