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JIP-2 antibody

Principal name

JIP-2 antibody

Alternative names for JIP-2 antibody

JNK-interacting protein 2, MAPK8IP2, IB2, JIP2, PRKM8IPL, JIP2, Islet-brain-2, C-jun-amino-terminal kinase-interacting protein 2, JNK MAP kinase scaffold protein 2, Mitogen-activated protein kinase 8-interacting protein 2

SwissProt ID

Q13387 (Human), Q9ERE9 (Mouse)

Gene ID

23542 (MAPK8IP2), 60597 (Mapk8ip2)

Ncbi ID

9606, NP_036456.1, NP_036456, NP_068740.3, NM_021921, NM_016431

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Frozen Sections (C)

Background of JIP-2 antibody

c-Jun NH2-terminal kinases (JNKs) are distant members of the MAP kinase family. JNK1 is activated by dual phosphorylation at a Thr-Pro-Tyr motif in response to ultraviolet (UV) light, and it functions to phosphorylate c-Jun at amino terminal serine regulatory sites, Ser 63 and Ser 73, resulting in transcriptional activation. Two additional JNK family members have been identified as JNK2 and JNK3. JIP-1 (for JNK interacting protein-1) has been identified as a cytoplasmic inhibitor of JNK that retains JNK in the cytoplasm, thereby inhibiting JNK-regulated gene expression. Evidence suggests that JNK1 and JNK2 bind to JIP-1 with greater affinity than to ATF-2 and c-Jun, which are targets of the JNK signaling pathway. JIP-1 contains an amino terminal JNK binding domain and a carboxy terminal SH3 domain. ATF-2 and c-Jun also contain the JNK binding domain and are thought to compete with JIP-1 for JNK binding. Multiple splice variants of JIP-1, including JIP-1b, JIP-1c (also designated islet-brain 1 or IB-1), JIP-2a, JIP-2b and JIP-3, have been identified in brain.

Immunohistochemistry (IHC) analyzes of JIP-2 antibody (Cat.-No.: AP06724PU-N) in paraffin-embedded human brain tissue.

AP06724PU-N

Western blot (WB) analysis of JIP-2 antibody (Cat.-No.: AP06724PU-N) in extracts from raw264.7 cells.

AP06724PU-N

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using JIP2 Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using JIP2 Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from mouse brain cells, using JIP2 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemical analysis of JIP2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of JIP2 staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of JIP2 expression in Raw264.7 (A); mouse brain (B) whole cell lysates.

Adult rat brain membrane immunoblot

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Ttissue and transfected cell immunoblot: extracts of adult rat brain membrane RBM and COS cells transiently transfected with Flag-tagged JIP-1, JIP-2 or untagged Kv2.1 plasmid and probed with N135/37 (left), mouse anti-Flag (middle) and N52A/42 (right).

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Adult rat brain immunohistochemistry

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Immunoblot versus extracts from various WT (+/+) and IB2 KO (-/-) mouse brain regions probed with N135/37. Data courtesy of Mitchell Goldfarb (Hunter College) and reproduced with permission from the Journal of Neuroscience (2010 Giza et al, PMID 21048139).

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Adult rat brain membrane immunoblot

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Adult rat brain immunohistochemistry

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Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
73-163	JIP-2 antibody
	Mouse	IgG1	N135/37	Supernatant	Hu, Ms, Rt	C, ICC/IF, WB	5 ml / €460.00
							Antibodies Incorporated
+3 additional images	Highly validated antibody!
75-163	JIP-2 antibody
	Mouse	IgG1	N135/37	Purified	Hu, Ms, Rt	C, ICC/IF, WB	0.1 ml / €540.00
							Antibodies Incorporated
+3 additional images	Highly validated antibody!
AM60064PU-N	JIP-2 (226-421) antibody
	Mouse	IgG1	S135-37	Purified	Ms, Rt	ICC/IF, P, WB	0.1 mg / €380.00
							Acris Antibodies GmbH
AP06724PU-N	JIP-2 antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	ICC/IF, P, WB	0.1 mg / €350.00
							Acris Antibodies GmbH
+1 additional image
C10973-1	JIP-2 antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	E, ICC/IF, P, WB	50 µg / €230.00
							Assay Biotech
+2 additional images
C10973-2	JIP-2 antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	E, ICC/IF, P, WB	0.1 mg / €330.00
							Assay Biotech
+2 additional images

CRISPR

Catalog No.	Species	Donor Vector
KN206272	MAPK8IP2 - human gene knockout kit via CRISPR
	Human	GFP-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN206272BN	MAPK8IP2 - human gene knockout kit via CRISPR
	Human	mBFP-Neo	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN206272LP	MAPK8IP2 - human gene knockout kit via CRISPR
	Human	Luciferase-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN206272RB	MAPK8IP2 - human gene knockout kit via CRISPR
	Human	RFP-BSD	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN309770	Mapk8ip2 - mouse gene knockout kit via CRISPR
	Mouse	GFP-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN309770BN	Mapk8ip2 - mouse gene knockout kit via CRISPR
	Mouse	mBFP-Neo	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN309770LP	Mapk8ip2 - mouse gene knockout kit via CRISPR
	Mouse	Luciferase-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN309770RB	Mapk8ip2 - mouse gene knockout kit via CRISPR
	Mouse	RFP-BSD	1 kit / €1,290.00
			OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.
RC219089L2V	Lenti ORF particles, MAPK8IP2 (mGFP-tagged) - Human mitogen-activated protein kinase 8 interacting protein 2 (MAPK8IP2), transcript variant 2, 200 uL, >10^7 TU/mL
		200 µl / €1,772.00
		OriGene Technologies, Inc.

RC219089L1V	Lenti ORF particles, MAPK8IP2 (Myc-DDK tagged) - Human mitogen-activated protein kinase 8 interacting protein 2 (MAPK8IP2), transcript variant 2 , 200 uL, >10^7 TU/mL
		200 µl / €1,597.00
		OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR210859L2V	Lenti ORF particles, Mapk8ip2 (GFP-tagged) - Mouse mitogen-activated protein kinase 8 interacting protein 2 (Mapk8ip2), 200 uL, >10^7 TU/mL
		200 µl / €1,607.00
		OriGene Technologies, Inc.

MR210859L1V	Lenti ORF particles, Mapk8ip2 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase 8 interacting protein 2 (Mapk8ip2), 200 uL, >10^7 TU/mL
		200 µl / €1,442.00
		OriGene Technologies, Inc.

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