JMJD2B / KDM4B antibody

Principal name

JMJD2B / KDM4B antibody

Alternative names for JMJD2B / KDM4B antibody

JHDM3B, KIAA0876, JmjC domain-containing histone demethylation protein 3B, Lysine-specific demethylase 4B, Jumonji domain-containing protein 2B

SwissProt ID

O94953 (Human), Q91VY5 (Mouse)

Gene ID

23030 (KDM4B), 193796 (Kdm4b)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Paraffin Sections (P), Flow Cytometry (F), Frozen Sections (C)

Background of JMJD2B / KDM4B antibody

JMJD2B is a 1,064 amino acid protein encoded by the human gene JMJD2B. JMJD2B belongs to the JMJD2B histone demethylase family and contains one JmjC domain, one JmjN domain, two PHD-type zinc fingers and two Tudor domains. The two Tudor domains recognize and bind methylated histones and have an interdigitated structure; the unusual fold is required for its ability to bind methylated histone tails. JMJD2B is a histone demethylase that specifically demethylates Lys 9 residues of Histone H3, thereby playing a role in histone code. It does not demethylate Histone H3 Lys 4, H3 Lys 27, H3 Lys 36 or H4 Lys 20, however, and is only able to demethylate trimethylated H3 Lys-9 and has weaker activity than JMJD2A, JMJD2C and JMJD2D. JMJD2B demethyl-ation of Lysine residues will generate formaldehyde and succinate. JMJD2B is a ubiquitously expressed nuclear protein.

General readings

Beyer, S., et al. J. Biol. Chem. 283(52):36542-36552(2008)
Pollard, P.J., et al. Biochem. J. 416(3):387-394(2008)
Katoh, Y., et al. Int. J. Mol. Med. 20(2):269-273(2007)
Whetstine, J.R., et al. Cell 125(3):467-481(2006)
Gray, S.G., et al. J. Biol. Chem. 280(31):28507-28518(2005)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

JMJD2B / KDM4B antibody

Western blot analysis of JMJD2B in A20 cell lysate with JMJD2B antibody at (A) 1 and (B) 2 µg/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

JMJD2B / KDM4B antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using JHD3B antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

JMJD2B / KDM4B antibody

WB Suggested Anti-JMJD2B Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

JMJD2B / KDM4B antibody

WB Suggested Anti-JMJD2B Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human kidney Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

JMJD2B / KDM4B antibody

WB using the antibody against JMJD2b diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. Rabbit IgG Purified Hu E
0.1 ml / €325.00
  OriGene Technologies, Inc.

JMJD2B / KDM4B Control Peptide

JMJD2B / KDM4B
50 µg / €160.00
  Acris Antibodies GmbH

JMJD2B / KDM4B (N-term) antibody

Western blot analysis of JMJD2B Antibody (N-term) Cat.-No AP52268PU-N in A375 cell line lysates (35ug/lane). This demonstrates the JMJD2B antibody detected the JMJD2B protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

JMJD2B / KDM4B (N-term) antibody

Formalin-fixed and paraffin-embedded human Testis tissue reacted with JMJD2B Antibody (N-term) Cat.-No AP52269PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

JMJD2B / KDM4B antibody

Western blot analysis of Phospho-JMJD2B-pT305 Cat.-No AP52270PU-N in NIH-3T3 cell line lysates (35ug/lane). JMJD2B (arrow) was detected using the purified Pab. Rabbit Ig Aff - Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

JMJD2B / KDM4B antibody

Western Blot analysis of JMJD2B expression in transfected 293T cell line (H00023030-T01) by JMJD2B MaxPab polyclonal antibody.

Lane 1: JMJD2B transfected lysate(120.56 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

JMJD2B / KDM4B antibody

Western Blot analysis of JMJD2B expression in transfected 293T cell line (H00023030-T01) by JMJD2B MaxPab polyclonal antibody.

Lane 1: JMJD2B transfected lysate(120.56 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

JMJD2B / KDM4B antibody

ELISA was performed using a serial dilution of JMJD2B polyclonal antibody ( Cat # PAB14071 ). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 3,000. Rabbit Hu E, WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

JMJD2B / KDM4B antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using JHD3B Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, P
50 µg / €205.00
  Assay Biotech

JMJD2B / KDM4B antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using JHD3B Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, P
0.1 mg / €310.00
  Assay Biotech

JMJD2B / KDM4B antibody

Human kidney; WB Suggested Anti-JMJD2B Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human kidney; JMJD2B antibody - middle region (ARP58207_P050) in Human kidney cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu WB
50 µg / €350.00
  AVIVA Systems Biology

JMJD2B / KDM4B antibody

Human HepG2; WB Suggested Anti-JMJD2B Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; JMJD2B antibody - middle region (ARP58398_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Can, Hu WB
50 µg / €350.00
  AVIVA Systems Biology

JMJD2B / KDM4B antibody

JMJD2B / KDM4B Rabbit IgG Aff - Purified Ms WB
50 µg / €680.00
  Funakoshi Co., Ltd.

JMJD2B / KDM4B antibody

Western Blot: JMJD2B Antibody [NB100-74604] - Detection of Human JMJD2B by Western Blot of  Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP,  20% of IP loaded) from HeLa cells. Antibodies: Affinity purified  rabbit anti-JMJD2B antibody NB100-74604 used for IP at 3  mcg/mg lysate. JMJD2B was also immunoprecipitated by rabbit  anti-JMJD2B antibody NB100-74605, which recognizes a  downstream epitope. Detection: Chemiluminescence with an exposure time of 3  seconds. Rabbit IgG Aff - Purified Hu IP
0.1 mg / €440.00
  Novus Biologicals Inc.

JMJD2B / KDM4B antibody

Western Blot and  Immunoprecipitation: JMJD2B Antibody [NB100-74605] - Detection of Human JMJD2B on HeLa whole cell lysate using NB100-74605. JMJD2B was also  immunoprecipitated by rabbit anti-JMJD2B antibody NB100-74604. Rabbit IgG Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

JMJD2B / KDM4B antibody

JMJD2B / KDM4B Rabbit IgG Aff - Purified Hu E, P
0.1 mg / €360.00
  Novus Biologicals Inc.

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