JMJD2C / KDM4C antibody

Principal name

JMJD2C / KDM4C antibody

Alternative names for JMJD2C / KDM4C antibody

GASC1, JHDM3C, KIAA0780, GASC-1 protein, Lysine-specific demethylase 4C, Gene amplified in squamous cell carcinoma 1 protein, JmjC domain-containing histone demethylation protein 3C, Jumonji domain-containing protein 2C

SwissProt ID

Q8VCD7 (Mouse), Q9H3R0 (Human)

Gene ID

23081 (KDM4C)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse)

Available hosts

Rabbit, Mouse

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Frozen Sections (C)

Background of JMJD2C / KDM4C antibody

Jumonji, AT-rich interactive domain 1C (JARID1C) protein belongs to the highly conserved ARID protein family, which is involved in chromatin remodeling and transcriptional regulation during cell growth, differentiation, and development. In human this gene plays an important role in normal brain development. the bioinformatic prediction indicated several conserved domains incudind an ARID domain, a JmjC domain a C5HC2 zinc finger domain and a JmJ domain. The epigenetic information encoded as methylated CpG dinucleotides to the transcriptional machinery is transferred by a multifunction methylated DNA binding protein called Methylated CpG biding protein 2.
Differential expression of various genes has been studied in esophageal squamous cell carcinomas (ESCs) using genomic hybridization studies. Frequent amplification of DNA copy number has been reported at chromosome 9p23-24 in ESCs that is rich in oncogenes and other tumor-associated genes. One of the novel gene over-expressed in ESCs was cloned and designated as GASC1 or JmjC. The protein contains 2 PD-finger motifs and a P domain. The PHD domains are characteristics of nuclear proteins that participate in chromatin-mediated transcriptional regulation and are present in number of oncogenes (1).
Jumonji3C is amj3C is 1056 amino acid protein abundantly expressed in brain in gonads suggesting its role in brain and gonads functions. Jumonj3C is a histone demthylase and it demethylates Lys-9 and Lys-36 resdues on histone H3 and thus play an important and central role n histone code. Interesting ly, the Jumonji3C does not demthylates histone H3 at position Lys-4, H3 lys-27 nor does it catalyze H4 Lys-20. Jmj3C demthylates trimethylated H3 Lys-9 and Lys-36 residues while it has no activity on mono and dimethylated residues. The enzymatic demethylation of lysine residues generated formaldehyde and succinate. The enzyme also binds to 1 mole of iron as a cofactor, JmjC domain belongs to the cupin susperfamily that posses protein hydroxylases that catalyze novel histone modifications (2). Jmj3C protein also contains 2 Tudor domains that recognizes and binds methylated histones, the double Tudor domain has in terdigitated structure and an unusual fold that is required for its ability to bind methylated histones by tails.

General readings

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010)
Suikki, H.E., et al. Prostate 70(8):889-898(2010)
Kantojarvi, K., et al. Psychiatr. Genet. 20(3):102-108(2010)
Liu, G., et al. Oncogene 28(50):4491-4500(2009)
Canova, C., et al. Cancer Res. 69(7):2956-2965(2009)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

JMJD2C / KDM4C antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-KDM4C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500567, Dilution 1:50) Mouse IgG1 OTI5B9 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

JMJD2C / KDM4C antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-KDM4C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500587) Mouse IgG1 OTI4B1 Purified Hu F, ICC/IF, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

JMJD2C / KDM4C antibody

Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-KDM4C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500588, Dilution 1:50) Mouse IgG1 OTI8A1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

JMJD2C / KDM4C antibody

Immunohistochemistry-Paraffin: JMJD2C Antibody (5B9) [NBP1-47815] - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-KDM4C mouse monoclonal antibody. (Dilution 1:50) Mouse 5B9 Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €620.00
  Novus Biologicals Inc.

JMJD2C / KDM4C antibody

Western Blot: JMJD2C Antibody - Western blot analysis of JMJD2C in HeLa nuclear extracts. Rabbit Purified Hu, Ms C, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

JMJD2C / KDM4C antibody

Host: Rabbit; Target Name: JMJD2C; Antibody Dilution: 1.0ug/ml; Sample Tissue: Jurkat cell lysate Rabbit IgG Purified Hu, Ms WB
50 µg / €325.00
  OriGene Technologies, Inc.

JMJD2C / KDM4C antibody

WB using the antibody against JMJD2c diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 120 kDa) is indicated on the right; the marker (in kDa) is shown on the left. The smaller fragment of approximately 92 kDa may represent a splicing variant. Rabbit IgG Purified Hu E
0.1 ml / €325.00
  OriGene Technologies, Inc.

JMJD2C / KDM4C antibody

Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against human KDM4D. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:12,750. Rabbit IgG Purified Hu E
0.1 ml / €325.00
  OriGene Technologies, Inc.

JMJD2C / KDM4C Control Peptide

JMJD2C / KDM4C
0.25 mg / €250.00
  Acris Antibodies GmbH

JMJD2C / KDM4C (502-520) antibody

JMJD2C / KDM4C Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

JMJD2C / KDM4C (C-term) antibody

Western blot analysis of JMJD2C Antibody (C-term) Cat.-No AP52272PU-N in K562 cell line lysates (35ug/lane). This demonstrates the JMJD2C antibody detected the JMJD2C protein (arrow). Rabbit Ig Aff - Purified Hu F, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

JMJD2C / KDM4C antibody

JMJD2C / KDM4C Rabbit Hu, Rt E, WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

JMJD2C / KDM4C antibody

Nuclear extracts of HeLa cells ( 40 µg ) were analysed by Western blot using JMJD2B polyclonal antibody ( Cat # PAB14071 ) diluted 1 : 1,000 in TBS-Tween containing 5% skimmed milk. Rabbit Hu E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

JMJD2C / KDM4C antibody

Human Jurkat; Host: Rabbit. Target Name: JMJD2C. Antibody Dilution: 1.0ug/ml. Sample Tissue: Jurkat cell lysate; JMJD2C antibody - C-terminal region (ARP32974_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Hu, Ms P, WB
50 µg / €350.00
  AVIVA Systems Biology

JMJD2C / KDM4C antibody

Western Blot: JMJD2C Antibody [NB110-38884] - Detection of Human JMJD2C. Samples:  Whole cell lysate (15 and 50 ug) from HeLa cells.  Antibodies:  Rabbit anti-JMJD2C antibody NB110-38884 used  for WB at 0.1 ug/ml. Rabbit Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

JMJD2C / KDM4C antibody

JMJD2C / KDM4C Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €360.00
  Novus Biologicals Inc.

JMJD2C / KDM4C antibody

JMJD2C / KDM4C Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €390.00
  Novus Biologicals Inc.

JMJD2C / KDM4C antibody

JMJD2C / KDM4C Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €390.00
  Novus Biologicals Inc.

JMJD2C / KDM4C antibody

JMJD2C / KDM4C Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €390.00
  Novus Biologicals Inc.

JMJD2C / KDM4C antibody

JMJD2C / KDM4C Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €390.00
  Novus Biologicals Inc.

Items 1 to 20 of 22 total

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