JMJD2D / KDM4D antibody

Principal name

JMJD2D / KDM4D antibody

Alternative names for JMJD2D / KDM4D antibody

JHDM3D, JmjC domain-containing histone demethylation protein 3D, Jumonji domain-containing protein 2D, Lysine-specific demethylase 4D

SwissProt ID

Q3U2K5 (Mouse), Q6B0I6 (Human)

Gene ID

55693 (KDM4D)

Available reactivities

Hu (Human), Mky (Monkey), Prim (Primate)

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of JMJD2D / KDM4D antibody

Jumonji, AT-rich interactive domain 1C (JARID1C) protein belongs to the highly conserved ARID protein family, which is involved in chromatin remodeling and transcriptional regulation during cell growth, differentiation, and development. In human this gene plays an important role in normal brain development. the bioinformatic prediction indicated several conserved domains incudind an ARID domain, a JmjC domain a C5HC2 zinc finger domain and a JmJ domain. The epigenetic information encoded as methylated CpG di-nucleotides to the transcriptional machinery is transferred by a multifunction methylated DNA binding protein called Methylated CpG biding protein 2.
Jumonji domain containing 2D protein (Jmj2D) is a histone demethylase that specifically demethylates’Lys-9’ of histone H3, thereby playing a central role in histone code. Jmj2D does not demethylate histone H3 at’Lys-4’, H3 at’Lys-27’,H3 at ’Lys-36’ nor H4 at ’Lys-20’. Jmj2D demethylates both di- and trimethylated H3 ’Lys-9’ residue, while it has no activity on monomethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Differential expression of various genes has been studied in esophageal squamous cell carcinomas (ESCs) using genomic hybridization studies. Frequent amplification of DNA copy number has been reported at chromosome 9p23-24 in ESCs that is rich in oncogenes and other tumor-associated genes. One of the novel gene over-expressed in ESCs was cloned and designated as GASC1 or JmjC. The protein contains 2 PD-finger motifs and a P domain. The PHD domains are characteristics of nuclear proteins that participate in chromatin-mediated transcriptional regulation and are present in number of oncogenes (1). Jumonji domain containing protein 2D is a amino acid protein abundantly expressed in brain in gonads suggesting its role in brain and gonads functions. Jumonj3C is a histone demethylase and it demethylates Lys-9 and Lys-36 residues on histone H3 and thus play an important and central role n histone code. Interesting ly, the Jumonji3C does not demethylates histone H3 at position Lys-4, H3 lys-27 nor does it catalyze H4 Lys-20. Jmj3C demethylates trimethylated H3 Lys-9 and Lys-36 residues while it has no activity on mono and dimethylated residues. The enzymatic demethylation of lysine residues generated formaldehyde and succinate. The enzyme also binds to 1 mole of iron as a cofactor, JmjC domain belongs to the cupin susperfamily that posses protein hydroxylases that catalyze novel histone modifications (2). Jmj3C protein also contains 2 Tudor domains that recognizes and binds methylated histones, the double Tudor domain has in terdigitated structure and an unusual fold that is required for its ability to bind methylated histones by tails.

General readings

Ralf Janknecht, et al., (2007) Biochem Biophy Res Comm 359:742

14 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

JMJD2D / KDM4D antibody

Detection of JMJD2D in HeLa nuclear extracts using anti-JMJD2D. Rabbit Purified Hu, Prim WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

JMJD2D / KDM4D antibody

WB Suggested Anti-JMJD2D Antibody Titration: 5.0 ug/ml; ELISA Titer: 1:62500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

JMJD2D / KDM4D antibody

WB Suggested Anti-JMJD2D Antibody Titration: 0.2-1 ug/ml; Positive Control: Human brain Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

JMJD2D / KDM4D control peptide

JMJD2D / KDM4D
0.25 mg / €250.00
  Acris Antibodies GmbH

JMJD2D / KDM4D (N-term) antibody

JMJD2D / KDM4D Rabbit Aff - Purified Hu, Mky E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

JMJD2D / KDM4D (C-term) antibody

Figure 1. Western blot analysis of anti-JMJD2D (C-term) Pab in Hela cell line lysate. JMJD2D (C-term)(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

JMJD2D / KDM4D antibody

Western Blot analysis of JMJD2D expression in transfected 293T cell line (H00055693-T01) by JMJD2D MaxPab polyclonal antibody.

Lane 1: JMJD2D transfected lysate(58.60 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

JMJD2D / KDM4D antibody

Western blot analysis of JMJD2D polyclonal antibody ( Cat # PAB2197 ) in Hela cell line lysate. JMJD2D ( C-term ) ( arrow ) was detected using the purified polyclonal antibody. Rabbit Hu E, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

JMJD2D / KDM4D antibody

Western blot analysis of JMJD2D in HeLa nuclear extracts using JMJD2D polyclonal antibody ( Cat # PAB12409 ). Rabbit Hu WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

JMJD2D / KDM4D antibody

Human HepG2; WB Suggested Anti-JMJD2D Antibody Titration: 5.0ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate; JMJD2D antibody - C-terminal region (ARP35946_T100) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Hu WB
0.1 mg / €310.00
  AVIVA Systems Biology

JMJD2D / KDM4D antibody

Human Intestine; JMJD2D antibody - C-terminal region (ARP39325_P050) in Human Intestine cells using Immunohistochemistry Rabbit Aff - Purified Hu P, WB
50 µg / €350.00
  AVIVA Systems Biology

JMJD2D / KDM4D antibody

JMJD2D / KDM4D Rabbit Aff - Purified Hu, Mky WB
25 µl / €160.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

JMJD2D / KDM4D

JMJD2D / KDM4D Human Purified
2 µg / €300.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

KDM4D overexpression lysate

KDM4D overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

14 Item(s)

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