Kanamycin kinase type II antibody

Principal name

Kanamycin kinase type II antibody

Alternative names for Kanamycin kinase type II antibody

APH(3')-II, neo, kan, nptII, Aminoglycoside 3'-phosphotransferase, Neomycin-kanamycin phosphotransferase type II

SwissProt ID

P00552 (Klepn)

Ncbi ID


Available reactivities

Hu (Human), Bac (Bacteria)

Available hosts


Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB)

Background of Kanamycin kinase type II antibody

Neomycin phosphotransferase II (nptII) gene is used in selection of transformed organisms. It was initially isolated from the transposon Tn5 that was present in the bacterium strain Escherichia coli K12. The gene codes for the aminoglycoside 3'-phosphotransferase (denoted aph(3')-II or NPTII) enzyme. NPTII is probably the most widely used selectable marker for plant transformation. It is also used in gene expression and regulation studies in different organisms in part because N-terminal fusions can be constructed that retain enzymatic activity. In animal cells, G418 and neomycin are used as selectable agents.

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