KAT2A / GCN5L2 antibody

Principal name

KAT2A / GCN5L2 antibody

Alternative names for KAT2A / GCN5L2 antibody

Histone acetyltransferase KAT2A, Histone acetyltransferase GCN5, HsGCN5, HGCN5, Lysine acetyltransferase 2A, STAF97, General control of amino acid synthesis protein 5-like 2

SwissProt ID

Q92830 (Human), Q9JHD2 (Mouse)

Gene ID

2648 (KAT2A), 264895 (Acsf2), 14534

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F)

Background of KAT2A / GCN5L2 antibody

GCN5 (general control of amino-acid synthesis 5) is a 94 kD member of the N-acetyltransferase superfamily. This nuclear protein is highly expressed in the liver where it functions as a transcriptiol co-activator, transfering acetyl group from acetyl-CoA to epsilon amino acid of lysine 14 in core histone H3 to control chromatin remodeling during transcriptiol activation. GCN5 is a component of the human STAGA transactivator complex (SPT3, TAF, and GCN5) and can be modified by phosphorylation (which inhibits activity). In addition to the STAGA complex proteins, GCN5 has been shown to interact with E1A and Myc. The Poly6073 antibody has been shown to be useful for Western blotting of the human GCN5 protein. The Poly6073 antibody is directed against the human GCN5 N-terminus.

General readings

Terreni, M., et al. Retrovirology 7, 18 (2010)
Atanassov, B.S., et al. Mol. Cell 35(3):352-364(2009)
Kelly, T.J., et al. J. Biol. Chem. 284(30):19945-19952(2009)
Mao, X., et al. Genes Dev. 23(7):849-861(2009)
Paolinelli, R., et al. Nat. Struct. Mol. Biol. 16(4):412-420(2009)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

KAT2A / GCN5L2 antibody

Immunofluorescence of HeLa cells were stained by monoclonal anti-human GCN5L2 antibody (1:500) with Alexa 488 (Green). Mouse IgG2a AT3G13 Purified Hu E, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

KAT2A / GCN5L2 antibody

Immunofluorescence of HeLa cells were stained by monoclonal anti-human GCN5L2 antibody (1:500) with Alexa 488 (Green). Mouse IgG2a AT3G13 Purified Hu E, ICC/IF, WB
50 µl / €230.00
  Acris Antibodies GmbH

KAT2A / GCN5L2 antibody

Western blot analysis of extracts from mouse brain cells, using GCN5L2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

KAT2A / GCN5L2 antibody

MCF-7 nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-GCN5 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and chemiluminescence detection system. Rabbit IgG Poly6072 Purified Hu ICC/IF, WB
50 µl / €325.00
  OriGene Technologies, Inc.

KAT2A / GCN5L2 antibody

HepG2 nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-GCN5 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Poly6073 Purified Hu P, WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

KAT2A / GCN5L2 antibody

Western blot (WB) analysis of GCN5 antibody (Cat.-No.: AP01242PU-N) in extracts from mouse brain. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

KAT2A / GCN5L2 (N-term) antibody

Western blot analysis of anti-GCN5 Antibody (N-term) (Cat.#AP11102PU-N) in 293 cell line lysates (35ug/lane). GCN5 (arrow) was detected using the purified Pab (1:60 dilution). Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

KAT2A / GCN5L2 (C-term) antibody

Flow cytometric analysis of Neuro-2a cells using KAT2A / GCN5L2 Antibody (C-term) Cat.-No AP52039PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis. Rabbit Ig Purified Hu, Ms F, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

KAT2A / GCN5L2 (C-term) antibody

Western blot analysis of KAT2A / GCN5L2 Antibody (C-term) Cat.-No AP52291PU-N in UM cell line lysates (35ug/lane). This demonstrates the KAT2A antibody detected the KAT2A protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

KAT2A / GCN5L2 antibody

Immunofluorescence of monoclonal antibody to GCN5L2 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG2b 4D3 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

KAT2A / GCN5L2 antibody

GCN5L2 monoclonal antibody (M03), clone 4F9 Western Blot analysis of GCN5L2 expression in MCF-7 ( Cat # L046V1 ). Mouse IgG2a 4F9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

KAT2A / GCN5L2 antibody

Immunoperoxidase of monoclonal antibody to GCN5L2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml] Mouse IgG2b 3F8 Purified Hu, Rt E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

KAT2A / GCN5L2 antibody

Western blot analysis of GCN5L2 polyclonal antibody ( Cat # PAB2244 ) in 293 cell line lysates ( 35 µg/lane ) . GCN5 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) . Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

KAT2A / GCN5L2 antibody

Western blot analysis of extracts from mouse brain cells, using GCN5L2 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €205.00
  Assay Biotech

KAT2A / GCN5L2 antibody

Western blot analysis of extracts from mouse brain cells, using GCN5L2 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €310.00
  Assay Biotech

KAT2A / GCN5L2 antibody

Immunohistochemical analysis of GCN5 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Chk, Hu, Ms, Rt P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

KAT2A / GCN5L2 antibody

Immunohistochemical analysis of GCN5 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Chk, Hu, Ms, Rt P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

KAT2A / GCN5L2 antibody

Immunohistochemical analysis of GCN5 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Chk, Hu, Ms, Rt P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

KAT2A / GCN5L2 antibody

Hela cells stained with purified rabbit polyclonal antibody against GCN5 (poly6072), followed by Rhodamine Red-X conjugated goat anti-rabbit IgG and DAPI Rabbit IgG Aff - Purified Hu, Ms WB
0.2 ml / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

KAT2A / GCN5L2 (411-837, His-tag)

KAT2A / GCN5L2 Human Purified > 90 % E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

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