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KCNJ2 antibody

Principal name

KCNJ2 antibody

Alternative names for KCNJ2 antibody

KCNJ2, HIRK1, Inward rectifier K(+) channel Kir2.1, Cardiac inward rectifier potassium channel, IRK1, Inward rectifier potassium channel 2, Potassium channel, inwardly rectifying subfamily J member 2

SwissProt ID

O19182 (Bovin), P35561 (Mouse), P63252 (Human), Q64273 (Rat)

Gene ID

3759 (KCNJ2), 16518 (Kcnj2), 29712

Ncbi ID

9606, NP_000882, NP_000882.1, NP_032451.1, NM_008425, NM_017296, NM_000891

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit, Chicken

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of KCNJ2 antibody

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces.
There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligandgated, other gating systems and finally those that are classified differently, having more exotic characteristics.
The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2).
The Kir2.1 inward-rectifier potassium ion channel is encoded by the KCNJ2 gene. A defect in this gene is associated with Andersen-Tawil syndrome (3).

General readings

Hinard V. et al. Initiation of human myoblast differentiation via dephosphorylation of Kir2.1 K+ channels at tyrosine 242. Development, Mar 2008; 135: 859 – 867.

KCNJ2 polyclonal antibody (A01), Lot # 051011JC01. Western Blot analysis of KCNJ2 expression in Daoy.

H00003759-A01

(LEFT) Western blot analysis of KCNJ2 Antibody (C-term) (AP17516PU-N) in NCI-H460 cell line lysates (35ug/lane). KCNJ2 (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of KCNJ2 Antibody (C-term) (AP17516PU-N) in mouse lung tissue lysates (35ug/lane). KCNJ2 (arrow) was detected using the purified Pab.

AP17516PU-N

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using KCNJ2 Antibody. The picture on the right is treated with the synthesized peptide.

Kir2.1 K+ Channel western blots of Rat Brain Membranes (RBM) and COS cells transiently transfected with HA-tagged Kir2.1 or untagged Kv2.1 plasmids.

MO50013-100

Kir2.1 K+ Channel staining of rat hippocampus.

MO50013-100

Western blot analysis of Kir2.1 expression in HeLa (A); mouse brain (B); rat kidney (C) whole cell lysates.

Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with HA-tagged Kir2.1 or untagged Kv2.1 plasmids and probed with N112B/14 (left) and K89/41 (right) TC supe.

75-210

Adult rat hippocampus immunohistochemistry

75-210

73-210

Adult rat hippocampus immunohistochemistry

73-210

Western blot analysis of Kir2.1 in COS transient cells using a 1:1000 dilution of the antibody

TA326486

IF analysis of Kir2.1 in mouse backskin sections. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.

TA326486

Western blot analysis of rat heart (1 and 3) and rat brain (2 and 4) membranes: 1, 2. Anti-Kir2.1 antibody , (1:200). 3, 4. Anti-Kir2.1, antibody, preincubated with the control peptide antigen.

TA328938

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA326486	KCNJ2 antibody
	Mouse Monoclonal Anti-Kir2.1 Antibody
	Mouse	IgG1	S21-32	Purified	Hu, Ms, Rt	C, WB	0.1 mg / €410.00
							OriGene Technologies, Inc.
+1 additional image
TA328938	KCNJ2 antibody
	Rabbit polyclonal Anti-Kir2.1
	Rabbit			Purified	Hu, Ms, Rt	WB	0.2 ml / €622.00
							OriGene Technologies, Inc.
73-210	KCNJ2 antibody
	Mouse	IgG1	N112B/14	Supernatant	Ms, Rt	ICC/IF, WB	5 ml / €460.00
							Antibodies Incorporated
+1 additional image
75-210	KCNJ2 antibody
	Mouse	IgG1	N112B/14	Purified	Ms, Rt	ICC/IF, WB	0.1 ml / €540.00
							Antibodies Incorporated
+1 additional image
AP17516PU-N	KCNJ2 (C-term) antibody
	Rabbit				Hu, Ms	WB	0.4 ml / €370.00
							Acris Antibodies GmbH
AP55033PU-N	KCNJ2 antibody
	Chicken			Purified	Hu	C, E, WB	50 µg / €300.00
							Acris Antibodies GmbH
AP55169SU-N	KCNJ2 antibody
	Rabbit			Serum	Hu	C, E, WB	0.2 ml / €400.00
							Acris Antibodies GmbH
C16400-1	KCNJ2 antibody
	Rabbit			Aff - Purified	Hu, Rt	E, P	50 µg / €230.00
							Assay Biotech
C16400-2	KCNJ2 antibody
	Rabbit			Aff - Purified	Hu, Rt	E, P	0.1 mg / €330.00
							Assay Biotech
MO50013-100	KCNJ2 (390-411) antibody
	Mouse	IgG1		Aff - Purified	Ms, Rt	ICC/IF, WB	0.1 ml / €490.00
							Neuromics Antibodies
+3 additional images

CRISPR

Catalog No.	Species	Donor Vector
KN308656	Kcnj2 - mouse gene knockout kit via CRISPR
	Mouse	GFP-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN308656BN	Kcnj2 - mouse gene knockout kit via CRISPR
	Mouse	mBFP-Neo	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN308656LP	Kcnj2 - mouse gene knockout kit via CRISPR
	Mouse	Luciferase-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN308656RB	Kcnj2 - mouse gene knockout kit via CRISPR
	Mouse	RFP-BSD	1 kit / €1,290.00
			OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.
RC217518L2V	Lenti ORF particles, KCNJ2 (mGFP-tagged) - Human potassium inwardly-rectifying channel, subfamily J, member 2 (KCNJ2), 200 uL, >10^7 TU/mL
		200 µl / €1,051.00
		OriGene Technologies, Inc.

RC217518L1V	Lenti ORF particles, KCNJ2 (Myc-DDK tagged) - Human potassium inwardly-rectifying channel, subfamily J, member 2 (KCNJ2) , 200 uL, >10^7 TU/mL
		200 µl / €938.00
		OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR221662L1V	Lenti ORF particles, Kcnj2 (Myc-DDK-tagged) - Mouse potassium inwardly-rectifying channel, subfamily J, member 2 (Kcnj2), 200 uL, >10^7 TU/mL
		200 µl / €804.00
		OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.
RR208941L2V	Lenti ORF particles, Kcnj2 (GFP-tagged ORF) - Rat potassium inwardly-rectifying channel, subfamily J, member 2 (Kcnj2), 200 uL, >10^7 TU/mL
		200 µl / €907.00
		OriGene Technologies, Inc.

RR208941L1V	Lenti ORF particles, Kcnj2 (Myc-DDK-tagged ORF) - Rat potassium inwardly-rectifying channel, subfamily J, member 2 (Kcnj2), 200 uL, >10^7 TU/mL
		200 µl / €804.00
		OriGene Technologies, Inc.

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