KEAP1 antibody

Principal name

KEAP1 antibody

Alternative names for KEAP1 antibody

KEAP1, INRF2, KIAA0132, KLHL19, Kelch-like ECH-associated protein 1, Cytosolic inhibitor of Nrf2, INrf2, Kelch-like protein 19

SwissProt ID

P57790 (Rat), Q14145 (Human), Q5R774 (Ponab), Q684M4 (Pig), Q9Z2X8 (Mouse)

Gene ID

9817 (KEAP1)

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), African clawed frog, Can (Canine), Ze (Zebrafish), Mky (Monkey), Eq (Equine), Rb (Rabbit), GP (Guinea Pig)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F), Frozen Sections (C)

Background of KEAP1 antibody

The nuclear factor-like 2 (NRF2) transcriptiol activator binds antioxidant response elements (ARE) of target gene promoter regions to regulate expression of oxidative stress response genes. Under basal conditions, the NRF2 inhibitor INrf2 (also called KEAP1) binds and retains NRF2 in the cytoplasm where it can be targeted for ubiquitin-mediated degradation. Small amounts of constitutive nuclear NRF2 maintains cellular homeostasis through regulation of basal expression of antioxidant response genes. Following oxidative or electrophilic stress, KEAP1 releases NRF2, thereby allowing the activator to translocate to the nucleus and bind to ARE-containing genes. The coordited action of NRF2 and other transcription factors mediates the response to oxidative stress. Altered expression of NRF2 is associated with chronic obstructive pulmory disease (COPD). NRF2 activity in lung cancer cell lines directly correlates with cell proliferation rates, and inhibition of NRF2 expression by siR enhances anti-cancer drug-induced apoptosis.The NRF2 repressor KEAP1 contains an amino termil BTB/POZ domain and a carboxyl termil KELCH domain. The KELCH domain is required for interacting with NRF2 and the BTB/POZ domain functions in binding Cul3 E3 ubiquitin ligase. Under normal conditions, the complex leads to the cytoplasmic sequestration and ubiquitin-mediated proteasomal degradation of NRF2. Electrophilic modification of KEAP1 leads to disassociation of the NRF2/KEAP1 complex. KEAP1 also targets the down regulation of NF-?B activity by targeting IKK¦Â degradation. Mutation of the corresponding KEAP1 gene is seen in lung cancer cases and can lead to uncontrolled activation of NRF2.

General readings

Zhang DD, Lo SC, Cross JV, et al. Keap1 is a redox-regulated substrate adaptor protein for a Cul3-dependent ubiquitin ligase complex. Mol. Cell. Biol. 2004;24:10941-53.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

KEAP1 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-KEAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502059) Mouse IgG1 OTI1B4 Purified Hu, Ms F, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

KEAP1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-KEAP1 Rabbit Polyclonal antibody. (TA590238) Rabbit IgG Purified Hu E, P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

KEAP1 (41-53) antibody

AP32137PU-N KEAP1 antibody staining of paraffin embedded Human Cerebellum at 3.8 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

KEAP1 (C-term) antibody

Western blot analysis of KEAP1 in human lung tissue lysate with KEAP1 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

KEAP1 antibody

Predicted band size: 70 kDa . Positive control: Jurkat cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

KEAP1 antibody

Western blot analysis of extracts of HepG2 cell lines, using KEAP1 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

KEAP1 antibody

WB Suggested Anti-KEAP1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

KEAP1 antibody

WB Suggested Anti-KEAP1 Antibody Titration: 1.0 ug/ml; Positive Control: HepG2 cell lysate KEAP1 is supported by BioGPS gene expression data to be expressed in HepG2 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

KEAP1 antibody

WB Suggested Anti-KEAP1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

KEAP1 antibody

Western blot analysis of Keap1 expression in NIH 3T3 lysate Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

KEAP1 antibody

Anti-KEAP1 mouse monoclonal antibody (TA501989) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KEAP1(RC202189). Mouse IgG1 OTI1G2 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

KEAP1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KEAP1 (RC202189, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KEAP1. Mouse IgG1 OTI1A1 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

KEAP1 (C-term) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle reacted with KEAP1 Antibody (C-term) Cat.-No AP52328PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

KEAP1 (C-term) Control Peptide

KEAP1
50 µg / €160.00
  Acris Antibodies GmbH

KEAP1 antibody

Human Kidney; Kidney; KEAP1 antibody - N-terminal region (ARP34727_P050) in Human Kidney cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

KEAP1 antibody

Human kidney; KEAP1 antibody - C-terminal region (ARP34728_T100) in Human kidney cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Por, Rt, Ze P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

KEAP1 antibody

Human Muscle; KEAP1 antibody - C-terminal region (ARP38981_P050) in Human Muscle cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Hu, Ms, Por, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

KEAP1 antibody

KEAP1 Rabbit IgG Aff - Purified Hu WB
0.1 mg / €290.00
  Boster Immunoleader

KEAP1 antibody

ELISA: KEAP1 Antibody [36540002] Rabbit Hu E, WB
50 µg / €400.00
  Novus Biologicals Inc.

KEAP1 antibody

Western Blot: KEAP1 Antibody [NBP1-78793] - 0.2ug/ml staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

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