Kelch-like protein 12 antibody

Principal name

Kelch-like protein 12 antibody

Alternative names for Kelch-like protein 12 antibody

KLHL12, C3IP1, CUL3-interacting protein 1

SwissProt ID

Q53G59 (Human), Q5U374 (Danre), Q6NRH0 (Xenla), Q8BZM0 (Mouse), Q8R2H4 (Rat)

Gene ID

59349 (KLHL12), 266772 (Klhl12)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Chicken, Mouse, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of Kelch-like protein 12 antibody

KLHL12 is a substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a negative regulator of Wnt sigling pathway and ER-Golgi transport. The BCR(KLHL12) complex is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitition of SEC31 (SEC31A or SEC31B). As part of the BCR(KLHL12) complex, also acts as a negative regulator of the Wnt sigling pathway by mediating ubiquitition and subsequent proteolysis of DVL3. The BCR(KLHL12) complex also mediates polyubiquitition of DRD4, without leading to degradation of DRD4.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Kelch-like protein 12 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-KLHL12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA803209) Mouse IgG2b OTI4E1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Kelch-like protein 12 antibody

Host: Rabbit; Target Name: KLHL12; Sample Tissue: Stomach Tumor lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Kelch-like protein 12 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KLHL12 (RC200894, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KLHL12. Mouse IgG1 OTI1E6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Kelch-like protein 12 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KLHL12 (RC200894, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KLHL12. Mouse IgG1 OTI4C10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Kelch-like protein 12 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KLHL12 (RC200894, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KLHL12. Mouse IgG1 OTI5C12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Kelch-like protein 12 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KLHL12 (RC200894, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KLHL12. Mouse IgG1 OTI1D2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Kelch-like protein 12 antibody

Western blot analysis using KLHL12 mouse mAb against Hela (1) cell lysate. Mouse IgG1 2G2 Ascites Hu E, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

Kelch-like protein 12 antibody

Western Blot analysis of KLHL12 expression in transfected 293T cell line (H00059349-T01) by KLHL12 MaxPab polyclonal antibody.

Lane 1: KLHL12 transfected lysate(62.48 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Kelch-like protein 12 (N-term) antibody

Rat Brain; WB Suggested Anti-Klhl12 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Rat Brain; Klhl12 antibody - N-terminal region (ARP39507_P050) in Rat Brain cells using Western Blot Rabbit Aff - Purified Hu, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

Kelch-like protein 12 antibody

Western Blot: E coli-derived fusion protein as test antigen. Affi-pure IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-KLHL12 ) Chicken Aff - Purified Hu, Ms, Rt ICC/IF, WB
50 µg / €520.00
  Novus Biologicals Inc.

Kelch-like protein 12 antibody

Western Blot: KLHL12/C3IP1  Antibody [NBP1-49927] - Detection of Human KLHL12 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-KLHL12 antibody used for IP at 3 mcg/mg lysate.  KLHL12 was also immunoprecipitated by rabbit anti-KLHL12 antibody  which recognizes a downstream epitope.  For blotting immunoprecipitated KLHL12 was used at 1 mcg/ml.  Detection: Chemiluminescence with an exposure time of 30 seconds. Rabbit Aff - Purified Hu IP
0.1 mg / €440.00
  Novus Biologicals Inc.

Kelch-like protein 12 antibody

Western Blot: C3IP1 Antibody (2G2) [NBP1-51616] - Western blot analysis using KLHL12 mouse mAb against Hela (1) cell lysate. Mouse IgG1 2G2 Purified Hu E, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

Kelch-like protein 12 antibody

Kelch-like protein 12 Rabbit Aff - Purified Hu, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Kelch-like protein 12

Kelch-like protein 12 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
2 µg / €300.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

KLHL12 293T Cell Transient Overexpression Lysate(Denatured)

KLHL12 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

15 Item(s)

per page
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