KIAA0226 antibody

Principal name

KIAA0226 antibody

SwissProt ID

Q80U62 (Mouse), Q92622 (Human)

Gene ID

9711 (KIAA0226), 100502698 (1700021K19Rik)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB), Immunoprecipitation (IP), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of KIAA0226 antibody

Two Beclin-1-interacting proteins, the run domain Beclin-1 interacting and cysteine-rich containing protein (Rubicon) and ATG14L, reciprocally regulate autophagy at different stages. Knockdown of Rubicon caused enhancement of autophagy while that of ATG14L caused a defect in autophagosome formation (1). Rubicon functions as part of a Beclin-1-PIK3C3-containing autophagy complex and is also an essential, positive regulator of the PDH oxidase complex (2). Upon microbial infection or TLR2 activation, Rubicon interacts with the CYBA subunit of the PDH oxidase complex, leading to a burst of reactive oxygen species and inflammatory cytokines (2).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

KIAA0226 antibody

Western blot analysis of Rubicon in 293 cell lysate with Rubicon antibody at 1 ug/mL. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

KIAA0226 antibody

KIAA0226 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

KIAA0226 antibody

KIAA0226 Rabbit IgG Aff - Purified Hu, Ms WB
50 µg / €680.00
  Funakoshi Co., Ltd.

KIAA0226 antibody

Immunoprecipitation: KIAA0226 Antibody [NBP1-30461]-Whole cell lysate (5, 15 and  50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells.  NBP1-30461 used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B)  and used for IP at 3 mcg/mg lysate. Rubicon was also  immunoprecipitated by another rabbit anti-Rubicon antibody,  which recognizes an upstream epitope. Rabbit Aff - Purified Hu IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

KIAA0226

KIAA0226 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

KIAA0226

KIAA0226 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

KIAA0226

KIAA0226 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

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