KIF11 antibody

Principal name

KIF11 antibody

Alternative names for KIF11 antibody

KIF11, EG5, KNSL1, TRIP5, Kinesin-like protein KIF11, Kinesin-related motor protein Eg5, Kinesin-like spindle protein HKSP, Thyroid receptor-interacting protein 5, TRIP-5, Kinesin-like protein 1

SwissProt ID

P52732 (Human), Q6P9P6 (Mouse), Q6S001 (Dicdi)

Gene ID

3832 (KIF11)

Available reactivities

Hu (Human), Mam (Mammalia), Mky (Monkey), Ms (Mouse), Por (Porcine), Rt (Rat), Bov (Bovine), Gt (Goat), Hst (Hamster), Sh (Sheep)

Available hosts

Rabbit, Mouse

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Flow Cytometry (F)

Background of KIF11 antibody

Eg5 (also known as kinesin-like protein KIF11, kinesin-related motor protein Eg5, kinesin-like spindle protein HKSP, and thyroid receptor interacting protein 5 (TRIP5)) is a 119 kD kinesin-like protein family, BimC subfamily. This protein is a catalytic kinesin motor with coiled-coil and Smc domains. Eg5 is localized at the centrosomes, spindle microtubules, and intracellular bridge. This motor protein is required for establishing the bipolar spindle. hEg5 is modified by phosphorylation on Thr927 by Cdc2 to allow association with the spindle apparatus. Eg5 has been shown to interact with the thyroid hormone receptor in presence of thyroid hormone and Cdc2.
Structure: Kinesin-like protein family, BimC subfamily, kinesin motor catalytic, coiled-coil, Smc domains; 119 kD
Distribution
: Centrosomes, spindle microtubules, intracellular bridge. 
Function: Motor protein required for establishing bipolar spindle. 
Modification: Phosphorylation.
Regulation: Phosphorylation on Thr927 by Cdc2 allows association with spindle apparatus.
Interaction: Thyroid hormone receptor in presence of thyroid hormone, Cdc2.

General readings

Tcherniuk,S., et.al., Biochem. Pharmacol. 79 (6), 864-872 (2010)
Parke,C.L., et.al., J. Biol. Chem. 285 (8), 5859-5867 (2010)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

KIF11 antibody

Western Blot: Eg5 Antibody TA301478 - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY KIF11 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KIF11. Rabbit IgG Purified Bov, Gt, Hst, Hu, Ms, Sh ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

KIF11 (N-term) antibody

Western blot analysis of KIF11 Antibody (N-term) (Cat. #TA325092) in CHO cell line lysates (35ug/lane). KIF11 (arrow) was detected using the purified Pab. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

KIF11 antibody

Hela cells were treated with 300 uM mimosine for 16 hrs, then placed in complete media (lane 1) or media containing 200 ng/ml nocodazole for an additional 18hrs. Cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit antibody against phosphorylated Eg5 (Thr927). Lane 1, Mimosine only treated Hela cells; Lane 2, mimosine and nocodazole treated Hela. Rabbit IgG Poly6205 Purified Hu ICC/IF, WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

KIF11 (200-500) antibody

Hela cell extract  (Lane 1) or NIH3T3 cell extract (Lane 2) was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-Eg5 (Clone 10C7/Eg5) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Mouse IgG1 10C7/Eg5 Aff - Purified Hu, Ms ICC/IF, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

KIF11 antibody

Western blot detection of Eg5 in MCF7,293T,Jurkat and Hela cell lysates using Eg5 mouse mAb (1:1000 diluted).Predicted band size:130KDa.Observed band size:130KDa. Mouse IgG1 4H3-1F12 Purified ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

KIF11 (1-1056) antibody

Immunoflourescence detection of KIF11 on acetone fixed HEp-2 cells with AM26646AF-N. Mouse IgG2b 2A5 Azide Free Hu ICC/IF, IP
0.1 mg / €390.00
  Acris Antibodies GmbH

KIF11 (1-1056) antibody

Western blot analysis of KIF11 expression in 293T using AM26647AF-N. Mouse IgG2a 3A1 Azide Free Hu IP, WB
0.1 mg / €390.00
  Acris Antibodies GmbH

KIF11 (200-500) antibody

Hela cell extract  (Lane 1) or NIH3T3 cell extract (Lane 2) was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-Eg5 (Clone 10C7/Eg5) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Mouse IgG1 10C7/Eg5 Aff - Purified Hu, Ms ICC/IF, IP, WB
25 µg / €210.00
  Acris Antibodies GmbH

KIF11 antibody

Immunohistochemistry analyzes of Eg5 antibody (AP20253PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

KIF11 antibody

KIF11 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

KIF11 antibody

Staining of KIF11 on HeLa cells fixed in 3.5% paraformaldehyde using KIF11 polyclonal antibody ( Cat # PAB12408 ). Cytoplasmic staining during interphase and spindle staining during mitosis. Rabbit Hu, Ms, Rt ICC/IF, IP, WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

KIF11 pThr926 antibody

Western blot analysis of extracts from COLO205 cells, using KIF11/Eg5 (Phospho-Thr926) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
50 µg / €205.00
  Assay Biotech

KIF11 pThr926 antibody

Western blot analysis of extracts from COLO205 cells, using KIF11/Eg5 (Phospho-Thr926) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
0.1 mg / €310.00
  Assay Biotech

KIF11 antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using KIF11/Eg5 (Ab-927) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

KIF11 antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using KIF11/Eg5 (Ab-927) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, P, WB
0.1 mg / €310.00
  Assay Biotech

KIF11 antibody

Western Blot and Immunoprecipitation: EG5/KIF11 Antibody [NB100-57514] - Detection of Human EG5/KIF11 on HeLa whole cell lysate using NB100-57514. EG5/KIF11 was also immunoprecipitated by rabbit anti-EG5/KIF11 antibody NB100-57513. Rabbit Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

KIF11 antibody

Immunofluorescence: Eg5 [p Thr927] Antibody [NB100-78409] - Overnight nocodazole treated Hela cells stained with purified rabbit polyclonal antibody against Phospho-Eg5 (Thr927), followed by Rhodamine Red-X conjugated goat anti-rabbit IgG and DAPI. against Aurora A, followed by Rhodamine Red-X conjugated goat anti-rabbit IgG and DAPI. Rabbit IgG Aff - Purified Hu ICC/IF, WB
50 µl / €320.00
  Novus Biologicals Inc.

KIF11 antibody

Hela cell extract (Lane 1) or NIH3T3 cell extract (Lane 2) was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-Eg5 (Clone 10C7/Eg5) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Mouse IgG1 10C7/Eg5 Aff - Purified Hu, Ms ICC/IF, IP, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

KIF11 antibody

KIF11 Rabbit Hu, Mam, Mky, Ms, Por, Rt ICC/IF, IP, WB
25 µl / €200.00
  Novus Biologicals Inc.

KIF11 antibody

KIF11 Rabbit IgG Aff - Purified Hu E, ICC/IF, P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

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