KIF5B antibody

Principal name

KIF5B antibody

Alternative names for KIF5B antibody

KIF5B, KNS, KNS1, Kinesin-1 heavy chain, Ubiquitous kinesin heavy chain, UKHC, Conventional kinesin heavy chain

SwissProt ID

P33176 (Human), Q2PQA9 (Rat)

Gene ID

3799 (KIF5B)

Available reactivities

Ms (Mouse), Hu (Human), Por (Porcine), Rt (Rat), Bov (Bovine), Mam (Mammalia), Can (Canine), Chk (Chicken), Ze (Zebrafish), African clawed frog, Sea Urchin, Rb (Rabbit), Xen (Xenopus), Eq (Equine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Enzyme Immunoassay (E), Frozen Sections (C), Immunoprecipitation (IP), Dot blot (Dot)

Background of KIF5B antibody

Kinesins are a superfamily of microtubule-based motor proteins involved in a variety of cellular processes including the transport of organelles in eukaryotic cells and cell division. They typically consist of 2 identical, approximately 110- to 120-kD heavy chains and 2 identical, approximately 60- to 70-kD light chains.
Kinesin has been found in a variety of organisms and cell types and is subject to spatial and temporal regulation. These proteins have a modular structure including a conserved motor domain of approximately 350 amino acids, which is responsible for microtubule binding and ATP hydrolysis. In addition to the motor domain, subfamily members share common domain organization, exhibit sequence similarity, motility properties, and cellular functions outside of the motor domain. There are currently three known Kinesin 5 family members denoted as A, B, and C. Kinesin 5A and kinesin 5C appear to be exclusively neuronal, whereas kinesin 5B appears to be ubiquitous in its expression.

General readings

General / background references:Brady ST et al. A monoclonal antibody against kinesin inhibits both anterograde and retrograde fast axonal transport in squid axoplasm. Proc Natl Acad Sci U S A 87:1061-5 (1990). PubMed PMID: 1689058
Yang JT et al. Evidence that the head of kinesin is sufficient for force generation and motility in vitro. Science 249:42-7 (1990). PubMed PMID: 2142332
Ingold AL et al. Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains. J Cell Biol 107:2657-67 (1988). PubMed PMID: 2974459
Brady ST A novel brain ATPase with properties expected for the fast axonal transport motor. Nature 317:73-5 (1985). PubMed PMID: 2412134

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

KIF5B (951-963) antibody

AP08832PU-N staining KIF5B at 2.5 µg/ml in paraffin-embedded Human uterus by Immunohistochemistry. Goat IgG Aff - Purified Bear, Bov, Can, Elephant, Eq, Hu, Mky, Platypus, Por, Prim, Rb, Tr C, E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

KIF5B antibody

Immunofluorescence staining of vesicles (red) in RBL-2H3 Rat basophilic leukemia cell line using anti-Kinesin Antibody Cat.-No SM3118P (Clone KN-03). Nuclei were stained with DAPI (blue). Mouse IgM KN-03 Purified Hu, Ms, Por, Rt ICC/IF, P, WB
0.1 mg / €260.00
  Acris Antibodies GmbH

KIF5B (C-term) antibody

TA302804 staining (0.2µg/ml) of Human Kidney lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, P, WB
20 µg / €200.00
  Acris Antibodies GmbH

KIF5B (C-term) antibody

TA302804 staining (0.2µg/ml) of Human Kidney lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

KIF5B antibody

Sample (30 ug of whole cell lysate). A: 293T. B: A431 . C: H1299. 15% SDS PAGE. TA308913 diluted at 1:1500. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

KIF5B antibody

WB Suggested Anti-KIF5B Antibody Titration: 0.2-1 ug/ml; Positive Control: Human brain Rabbit IgG Purified Bov, Can, Hu, Ms, Por, Rt, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

KIF5B antibody

WB Suggested Anti-KIF5B Antibody Titration: 0.2-1 ug/ml; Positive Control: Human brain Rabbit IgG Purified Bov, Can, Eq, Hu, Por, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

KIF5B antibody

KIF5B Mouse IgG2b H2 Purified Bov, Mam C, E, IP, P, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

KIF5B antibody

Detection of kinesin in reduced lysates of human HEK cell line (1) and porcine brain (2) by Rabbit polyclonal anti-Kinesin Antibody. Rabbit Purified Hu, Ms, Por ICC/IF, WB
0.1 mg / €260.00
  Acris Antibodies GmbH

KIF5B antibody

KIF5B Mouse IgG2a L2 Purified Bov, Mam E, WB
0.1 mg / €540.00
  Acris Antibodies GmbH

KIF5B antibody

Immunofluorescence staining of vesicles (red) in RBL-2H3 rat basophilic leukemia cell line using anti-Kinesin (KN-02). Nuclei were stained with DAPI (blue). Mouse IgM KN-02 Purified Hu, Ms, Por, Rt ICC/IF
0.1 mg / €260.00
  Acris Antibodies GmbH

KIF5B antibody

KIF5B Mouse IgG2a 2A11 Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

KIF5B antibody

Immunofluorescence staining of vesicles ( red ) in RBL-2H3 ( rat basophilic leukemia cell line ) using Kinesin (heavy chain) monoclonal antibody, clone KN-02 ( Cat # MAB3621 ). Nuclei were stained with DAPI ( blue ). Mouse IgM KN-02 Hu, Ms, Rt ICC/IF
0.1 mg / €390.00
  Abnova Taiwan Corp.

KIF5B antibody

Immunofluorescence staining of vesicles ( red ) in RBL-2H3 ( rat basophilic leukemia cell line ) using Kinesin ( heavy chain ) monoclonal antibody, clone KN-03 ( Cat # MAB3622 ). Nuclei were stained with DAPI ( blue ). Mouse IgM KN-03 Hu, Ms, Rt ICC/IF
0.1 mg / €390.00
  Abnova Taiwan Corp.

KIF5B antibody

KIF5B polyclonal antibody ( Cat # PAB6192 ) staining ( 1 µg/ml ) of paraffin embedded human kidney.  Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. Goat Hu E, P, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

KIF5B antibody

Human Liver; KIF5B antibody - N-terminal region (ARP33903_P050) in Human Liver cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze P, WB
50 µg / €350.00
  AVIVA Systems Biology

KIF5B antibody

Human Muscle; KIF5B antibody - C-terminal region (ARP33904_P050) in Human Muscle cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Can, Chk, Hu, Ms, Rt, Ze P, WB
50 µg / €350.00
  AVIVA Systems Biology

KIF5B antibody

NB 100-1059 staining (0.2µg/ml) of Human Kidney lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence Goat IgG Aff - Purified Can, Hu E, P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

KIF5B antibody

KIF5B Mouse IgM IBII Ascites Bov, Rt E, WB
0.1 ml / €360.00
  Novus Biologicals Inc.

KIF5B antibody

Immunofluorescence: Kinesin 5B Antibody (KN-01) [NB500-490] - Fig. 1. Immunofluorescence staining of 3T3 mouse fibroblast cell line. Double-labeled fluorescence reveals a co-distribution of vesicles with microtubules on the cell periphery. Vesicles (red) were stained with anti-Kinesin (KN-01), microtubules (green) with polyclonal anti-Tubulin antibody and nuclei with DAPI (blue). Mouse IgM KN-01 Aff - Purified Hu, Ms, Por ICC/IF
0.1 mg / €360.00
  Novus Biologicals Inc.

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