Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Paraffin Sections (P)
Background of Kinase suppressor of Ras 2 antibody
Kinase suppressor of Ras (KSR) and MAP kinase kinase kinase (MEKK3) are integral members of the MAP kinase pathway. Ksr is a conserved protein that positively regulates Ras signaling and may function as a scaffold for Raf, MEK and ERK. There are two types of Ksr proteins: Ksr-1 and Ksr-2. These two are individually necessary for a few specific Ras-dependent processes, but they are required together for most aspects of Ras-mediated signaling. Ksr-2 plays a key role in Ras-mediated signaling during germline meiotic progression and functions redundantly with Ksr-1 during the development of the excretory system pathway, hermaphrodite vulva and male spicules. Ksr-2 also functions as a negative regulator of the MEKK3-mediated activation of the MAP kinase pathways (specifically ERK and JNK) and of the NFκB pathways, and it simultaneously inhibits MEKK3-mediated IL-8 production.
Detection limit for recombinant GST tagged KSR2 is approximately 0.03ng/ml as a capture antibody.
Western blot analysis of KSR2 over-expressed 293 cell line, cotransfected with KSR2 Validated Chimera RNAi ( Cat # H00283455-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with KSR2 monoclonal antibody (M08) clone 1G4 (Cat # H00283455-M08 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
KSR2 Antibody (C-term) (Cat. #AP52428PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KSR2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
KSR2 Antibody (C-term) (Cat. #AP52428PU-N) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.