KITLG / SCF antibody

Principal name

KITLG / SCF antibody

Alternative names for KITLG / SCF antibody

KITL, Kit ligand, c-Kit ligand, Stem cell factor, Mast cell growth factor, MGF

SwissProt ID

P20826 (Mouse), P21581 (Rat), P21583 (Human), P79169 (Felca), P79368 (Sheep), Q06220 (Canfa), Q09108 (Chick), Q28132 (Bovin), Q29030 (Pig), Q95MD2 (Horse)

Gene ID

4254 (KITLG), 17311 (Kitl), 60427 (Kitlg)

Available reactivities

Hu (Human), Prim (Primate), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Rb (Rabbit), Eq (Equine), Sh (Sheep)

Available hosts

Mouse, Rabbit, Goat

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Functional assay (FN), Neutralisation (NEUT), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of KITLG / SCF antibody

Stem cell factor (SCF, kit ligand) is a type I membrane glycoprotein with 5 potential N-linked glycosylation sites. Soluble SCF is produced by proteolytic cleavage of the ECD. SCF is expressed primarily by fibroblasts however expression has also been detected in keratinocytes, mature granulocytes, Sertoli cells and bone marrow stromal cells. Functionally, SCF is involved in steady state maintenance of hematopoiesis and mediates the proliferation of myeloid, erythroid and lymphoid progenitors in bone marrow cultures. It has been shown to act synergistically with colony stimulating factors such as G-CSF and erythropoietin as well as interleukins. SCF is also involved in gametogenesis, melanogenesis and the membrane bound form of SCF mediates cell-cell adhesion. SCF contributes to the generation and survival of mast cells from CD34+ progenitor cells and plays a role in mast cell degranulation, resulting in the release of histamine, proinflammatory cytokines and chemokines. Furthermore, SCF also induces eosinophil activation. This suggests SCF is involved in allergic disease states and elevated levels of SCF are detected in inflammatory conditions in human and animal models such as asthma. The actions of SCF are exerted by binding to the receptor tyrosine kinase c-Kit (CD117), which results in receptor dimerization. Deregulated c-Kit signalling contributes to human tumours while inhibition of c-Kit signalling reduces histamine and mast cell levels and eosinophil infiltration. This is a HCX protein. HCX Expression System Details HCX proteins mimic the proteins in the human body because they are expressed from human, rather than animal, insect or bacterial cells. This process gives them human post-translational modifications. Recombinant DNA techniques allow a human protein with the correct amino acid sequence to be expressed in a non-human cell line. However, non-human cells lack the appropriate cellular machinery, such as specific glycosyltransferases, necessary to produce the correct human post-translational modifications of a protein. An extreme example is seen in E. coli cells, which produce recombinant proteins with no glycosylation, as the above figure illustrates. Rodent and yeast cells are able to glycosylate proteins, but they are still different from glycosylation in human cells. Expression System Resultant Proteins Human (e.g. K562, HEK293) Correct amino acid sequence Human post-translational modifications Rodent (e.g. CHO, NSO) Correct amino acid sequence Some natural glycosylation - not human-like Yeast (e.g. Pichia) Correct amino acid sequence Some natural glycosylation - not human-like E.Coli Correct amino acid sequence No PTMs Although there have been significant attempts to make non-human cell derived cytokines more human-like, there is a growing awareness that in many instances, particularly in therapeutics, cytokines should mimic those found in the body as closely as possible.

General readings

Zhang Z., et al. (2000). Proc. Natl. Acad. Sci. U.S.A. 97, 7732.Okada S , et al. (1992). Nippon Rinsho , 50, 1872.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

KITLG / SCF antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-KITLG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507209) Mouse IgG1 OTI5F6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

KITLG / SCF (C-term) antibody

Western blot analysis of KITLG Antibody (C-term) (Cat.#TA302195) in 293 cell line lysates (35ug/lane). KITLG (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu F, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

KITLG / SCF antibody

Western blot analysis of SCF in rat brain tissue lysate with SCF antibody at (A) 1 and (B) 2 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

KITLG / SCF antibody

Predicted band size: 31 kDa . Positive control: A549, A431, Hela and 3T3 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1,2,3: A549, A431, Hela and 3T3 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

KITLG / SCF antibody

To detect hSCF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hSCF. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

KITLG / SCF antibody

To detect hSCF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hSCF. Goat IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

KITLG / SCF antibody

To detect hSCF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human SCF (TA328438) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hSCF. Goat IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

KITLG / SCF antibody

To detect hSCF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human SCF (TA328437) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hSCF. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

KITLG / SCF antibody

To detect mSCF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mSCF. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

KITLG / SCF antibody

To detect mSCF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine SCF (TA328556) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSCF. Rabbit IgG Purified E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

KITLG / SCF antibody

To detect rat SCF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant rat SCF. Rabbit IgG Purified Rt E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

KITLG / SCF antibody

To detect rat SCF by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat SCF (TA328594) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant rat SCF. Rabbit IgG Purified Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

KITLG / SCF antibody

WB Suggested Anti-KITLG Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Can, Eq, Hu, Ms, Por, Rb, Rt, Sh P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

KITLG / SCF antibody

Western blot analysis of extracts from A549, A431, Hela and 3T3 cells using SCF Antibody Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

KITLG / SCF antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-KITLG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507211) Mouse IgG1 OTI6E5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

KITLG / SCF antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KITLG (RC216131, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KITLG. Mouse IgG1 OTI5E9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

KITLG / SCF antibody

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

KITLG / SCF antibody

Western Analysis of anti-Mouse SCF Cat.-No AP26030PU. Samples were loaded in 15% SDS-polyacrylamide gel under reducing conditions.
Lane 1: MWM (kDa); lane 2: rm SCF; lane 3: rh SCF. Rabbit IgG Purified Hu, Ms WB
0.2 mg / €370.00
  Acris Antibodies GmbH

KITLG / SCF antibody

Western Analysis of anti-Mouse SCF Cat.-No AP26030PU. Samples were loaded in 15% SDS-polyacrylamide gel under reducing conditions.
Lane 1: MWM (kDa); lane 2: rm SCF; lane 3: rh SCF. Rabbit IgG Purified Hu, Ms WB
0.1 mg / €270.00
  Acris Antibodies GmbH

KITLG / SCF Control Peptide

KITLG / SCF
50 µg / €160.00
  Acris Antibodies GmbH

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