KITLG / SCF antibody

Principal name

KITLG / SCF antibody

Alternative names for KITLG / SCF antibody

KITL, Kit ligand, c-Kit ligand, Stem cell factor, Mast cell growth factor, MGF

SwissProt ID

P20826 (Mouse), P21581 (Rat), P21583 (Human), P79169 (Felca), P79368 (Sheep), Q06220 (Canfa), Q09108 (Chick), Q28132 (Bovin), Q29030 (Pig), Q95MD2 (Horse)

Gene ID

4254 (KITLG), 17311 (Kitl), 60427 (Kitlg)

Available reactivities

Hu (Human), Prim (Primate), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Rb (Rabbit), Eq (Equine), Sh (Sheep)

Available hosts

Mouse, Rabbit, Goat

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Functional assay (FN), Neutralisation (NEUT), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of KITLG / SCF antibody

Stem Cell Factor is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors. This pleiotropic cytokine, alternately known as mast cell growth factor (MGF) and steel-factor (SLF), plays essential roles in gametogenesis, melanogenesis and early stages of hematopoiesis. In vitro and in vivo, SCF can stimulate the proliferation of mature, as well as the proliferation and maturation of immature, mast cells. On purified primitive human and mouse hematopoietic precursors, SCF acts in a synergistic manner with various growth factors, such as IL-1, IL-3, IL-6, IL-7, and Epo, to induce myeloid, erythroid and lymphoid lineage colony formation. The finding that SCF is also expressed in the nervous system suggests a possible role for SCF in the development of the nervous system. The cDNA sequences for human, mouse and rat SCF encode transmembrane proteins which are composed of a signal peptide, a 189 amino acid extracellular domain, a hydrophobic transmembrane domain and an intracellular domain. Native SCF can exist either as the membrane bound form or as a soluble form consisting of the first 164 or 165 amino acids of the extracellular domain. The soluble form is believed to be a proteolytic cleavage product of the transmembrane protein. Both the soluble and the transmembrane form of SCF have growth factor activities. Native soluble SCF is a heavily N- and O-glycosylated protein which exists as a non-covalently associated dimer in solution. All four cysteine residues of SCF monomers are involved in int ramolecular disulfide bonds.
Murine or rat soluble SCF is highly homologous to human soluble SCF (approximately 80%). Whereas both rat and mouse SCF are active on human cells, the human protein is much less active on mouse or rat cells.

General readings

Zhang Z., et al. (2000). Proc. Natl. Acad. Sci. U.S.A. 97, 7732.Okada S , et al. (1992). Nippon Rinsho , 50, 1872.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

KITLG / SCF antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-KITLG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507209) Mouse IgG1 OTI5F6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

KITLG / SCF (C-term) antibody

Western blot analysis of KITLG Antibody (C-term) (Cat.#TA302195) in 293 cell line lysates (35ug/lane). KITLG (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu F, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

KITLG / SCF antibody

Western blot analysis of SCF in rat brain tissue lysate with SCF antibody at (A) 1 and (B) 2 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

KITLG / SCF antibody

Predicted band size: 31 kDa . Positive control: A549, A431, Hela and 3T3 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1,2,3: A549, A431, Hela and 3T3 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

KITLG / SCF antibody

To detect hSCF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hSCF. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

KITLG / SCF antibody

To detect hSCF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hSCF. Goat IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

KITLG / SCF antibody

To detect hSCF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human SCF (TA328438) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hSCF. Goat IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

KITLG / SCF antibody

To detect hSCF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human SCF (TA328437) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hSCF. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

KITLG / SCF antibody

To detect mSCF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mSCF. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

KITLG / SCF antibody

To detect mSCF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine SCF (TA328556) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSCF. Rabbit IgG Purified E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

KITLG / SCF antibody

To detect rat SCF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant rat SCF. Rabbit IgG Purified Rt E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

KITLG / SCF antibody

To detect rat SCF by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat SCF (TA328594) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant rat SCF. Rabbit IgG Purified Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

KITLG / SCF antibody

WB Suggested Anti-KITLG Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Can, Eq, Hu, Ms, Por, Rb, Rt, Sh P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

KITLG / SCF antibody

Western blot analysis of extracts from A549, A431, Hela and 3T3 cells using SCF Antibody Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

KITLG / SCF antibody

Recombinant human SCF (lane 1, 50 ng) and mouse SCF (lane 2, 50 ng) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-human SCF (clone L061D3) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Mouse L061D3 Purified Hu, Ms WB
0.1 mg / €248.00
  OriGene Technologies, Inc.

KITLG / SCF antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-KITLG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507211) Mouse IgG1 OTI6E5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

KITLG / SCF antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KITLG (RC216131, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KITLG. Mouse IgG1 OTI5E9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

KITLG / SCF antibody

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

KITLG / SCF antibody

Western Analysis of anti-Mouse SCF Cat.-No AP26030PU. Samples were loaded in 15% SDS-polyacrylamide gel under reducing conditions.
Lane 1: MWM (kDa); lane 2: rm SCF; lane 3: rh SCF. Rabbit IgG Purified Hu, Ms WB
0.2 mg / €370.00
  Acris Antibodies GmbH

KITLG / SCF antibody

Western Analysis of anti-Mouse SCF Cat.-No AP26030PU. Samples were loaded in 15% SDS-polyacrylamide gel under reducing conditions.
Lane 1: MWM (kDa); lane 2: rm SCF; lane 3: rh SCF. Rabbit IgG Purified Hu, Ms WB
0.1 mg / €270.00
  Acris Antibodies GmbH

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