KLF15 antibody

Principal name

KLF15 antibody

Alternative names for KLF15 antibody

KLF15, KKLF, Krueppel-like factor 15, Kidney-enriched krueppel-like factor, CKLF, Cardiovascular Krueppel-like factor

SwissProt ID

Q9EPW2 (Mouse), Q9UIH9 (Human)

Gene ID

28999 (KLF15)

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Goat, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Dot blot (Dot)

Background of KLF15 antibody

The kidney chloride channel proteins CLCNKA and CLCNKB are highly related, although they are located in different areas of the kidney. Using a yeast 1-hybrid screen of a kidney cDNA library with the GA element of the CLCNKA promoter as bait, Uchida et al. (2000) isolated cDNAs encoding MAZ and KLF15, which they termed KKLF. Sequence analysis predicted that the 415-amino acid KLF15 protein, which is 84% identical to the rat Klf15 protein, contains 3 zinc finger motifs at its C terminus, N-terminal serine-rich stretches, and a central proline-rich segment. EMSA analysis confirmed that KLF15 and MAZ interact with the GA element of the CLCNKA promoter and showed that KLF15 binds with higher affinity and is a functional competitor of MAZ. Northern blot analysis revealed highest expression of a 2.5-kb KLF15 transcript in liver, followed by heart, skeletal muscle, and kidney. No expression was found in bone marrow or lymphoid tissues. Western blot analysis showed expression of a 50-kD protein. Immunohistochemical analysis detected nuclear expression of KLF15 in liver sinusoid stellate cells, subcapsular fibroblasts, and portal fibroblasts. KLF15 was also expressed in cardiac and skeletal muscle interstitial cells and in kidney inner medulla, glomeruli, and cortical interstitium. Immunofluorescence microscopy, however, demonstrated no colocalization of KLF15 with CLCNKA.

General readings

Uchida S et al. Transcriptional regulation of the CLC-K1 promoter by myc-associated zinc finger protein and kidney-enriched Kruppel-like factor, a novel zinc finger repressor. Mol Cell Biol 20:7319-31 (2000). PUBMED:10982849

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

KLF15 (N-term) antibody

TA302522 staining (0.3µg/ml) of HepG2 lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 12 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

KLF15 antibody

Western blot analysis of fetal liver lysates with anti-KLF15 antibody (1:1000). This antibody, under reducing conditions, identified a band of ~65kDa. Rabbit Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

KLF15 antibody

WB Suggested Anti-KLF15 Antibody Titration: 1.0-2.0ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

KLF15 antibody

WB Suggested Anti-Klf15 Antibody; Titration: 1.0 ug/ml; Positive Control: Mouse Brain Rabbit IgG Purified Hu, Ms WB
50 µg / €325.00
  OriGene Technologies, Inc.

KLF15 antibody

Immunofluorescence of purified MaxPab antibody to KLF15 on HepG2 cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

KLF15 antibody

KLF15 Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

KLF15 antibody

Western Blot analysis of KLF15 expression in transfected 293T cell line (H00028999-T02) by KLF15 MaxPab polyclonal antibody.

Lane 1: KLF15 transfected lysate(44.00 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

KLF15 antibody

KLF15 Mouse IgG2b 1F3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

KLF15 antibody

KLF15 polyclonal antibody ( Cat # PAB6181 ) staining ( 0.3 µg/ml ) of HepG2 lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 12 hour. Detected by western blot using chemiluminescence. Goat Hu, Ms E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

KLF15 antibody

Human Spleen; WB Suggested Anti-KLF15 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Spleen; KLF15 antibody - middle region (ARP31900_P050) in Human Spleen cells using Western Blot Rabbit Aff - Purified Hu, Ms, Por, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

KLF15 antibody

Human Liver; KLF15 antibody - N-terminal region (ARP32587_T100) in Human Liver cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

KLF15 antibody

Western Blot: KLF 15 Antibody [NB100-1047] - NB100-1047 Staining (0.3ug/ml) of HepG2 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

KLF15 antibody

Western blot : KLF15 Antibody (2G8 ) [NBP1-28874]- analysis using anti-KLF15 mAb against HepG2 (1) and SMMC-7721 (2) cell lysate. Mouse IgG1 2G8 Ascites Hu E, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

KLF15 antibody

Western Blot: KLF15 Antibody [NBP1-30923] - Western Blot analysis of KLF15 Antibody on MOLT4 (lane A) and Raji (lane B), diluted at 1:1000 with 10% SDS PAGE gel. Rabbit IgG Hu Dot, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

KLF15 antibody

Immunohistochemistry-Paraffin: KLF15 Antibody [NBP1-82872] - Immunohistochemical staining of human stomach shows cytoplasmic positivity in parietal cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

KLF15

KLF15 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

KLF15

KLF15 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

KLF15

KLF15 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

KLF15

KLF15 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

KLF15

KLF15 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

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