KLF4 antibody

Principal name

KLF4 antibody

Alternative names for KLF4 antibody

EZF, GKL, Krueppel-like factor 4, Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor

SwissProt ID

O43474 (Human), Q60793 (Mouse)

Gene ID

9314 (KLF4), 16600 (Klf4), 5793140 (apaH), 931 (MS4A1)

Available reactivities

Hu (Human), Ms (Mouse), Can (Canine), Chk (Chicken), Rb (Rabbit), Rt (Rat), African clawed frog, Bov (Bovine), Prim (Primate)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Flow Cytometry (F), Immunoprecipitation (IP), Frozen Sections (C)

Background of KLF4 antibody

KLF4 Monoclol Antibody: KLF4 is a transcription factor that functions as both a transcriptiol activator and repressor to regulate proliferation and differentiation of multiple cell types. The role of KLF4 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing KLF4 and the transcription factors POU5F1, Sox2, and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing KLF4, Sox2, POU5F1 and c-Myc, elimiting the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. KLF4 interacts directly with POU5F1 and Sox2 in iPS and ES cells and activates the target gene NOG.

General readings

1. Evans PM, Zhang W, Chen X, et al. Kruppel-like factor 4 is acetylated by p300 and regulates gene transcription via modulation of histone acetylation. J. Bio. Chem. 2007; 282:33994-4002.
2. Carpenter MK, Rosler E, and Rao MS. Characterization and differentiation of human embryonic stem cells. Cloning Stem Cells 2003; 5:79-88.
3. Takahashi K and Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 2006; 1263:663-76.
4. Okita K, Nakagawa M, Hyenjong H, et al. Generation of mouse induced pluripotent stem cells without viral vectors. Science 2008; 322:949-53.
5. Wei Z, Yang Y, Zhang P, et al. Klf4 interacts directly with Oct4 and Sox2 to promote reprogramming. Stem Cells 2009; 27:2969-78.
Carpenter MK, Rosler E, and Rao MS. Characterization and differentiation of human embryonic stem cells. Cloning Stem Cells 2003; 5:79-88.
Takahashi K and Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 2006; 1263:663-76.
Okita K, Nakagawa M, Hyenjong H, et al. Generation of mouse induced pluripotent stem cells without viral vectors. Science 2008; 322:949-53.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

KLF4 (N-term) antibody

Western blot analysis of KLF4 Antibody (N-term C74) (Cat. #TA324722) in Jurkat,293 cell line lysates (35ug/lane).KLF4 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu P
0.4 ml / €325.00
  OriGene Technologies, Inc.

KLF4 antibody

Western blot analysis of lysate from HT29 cell line, using KLF4 Antibody(Cat. #TA324724). TA324724 was diluted at 1:1000. A goat anti-rabbit (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug . Rabbit Ig Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

KLF4 antibody

Western blot analysis of KLF4-S457 (Cat.# TA324725) in MDA-MB231, NCI-H460 cell line lysates (35ug/lane). KLF4 (arrow) was detected using the purified Pab. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

KLF4 (1-170) antibody

Immunohistochemistry: Paraffin embedded sections of human gastric cancer tissue were incubated with anti-human KLF4 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer. Diaminobenzidine (DAB) was used for detection. Mouse IgG1 AT4E6 Purified Hu E, F, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

KLF4 (1-170) antibody

Immunohistochemistry: Paraffin embedded sections of human gastric cancer tissue were incubated with anti-human KLF4 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer. Diaminobenzidine (DAB) was used for detection. Mouse IgG1 AT4E6 Purified Hu E, F, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

KLF4 (N-term) antibody

TA302803 (0.1µg/ml) staining of Human Duodenum lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Rt E, WB
20 µg / €200.00
  Acris Antibodies GmbH

KLF4 (N-term) antibody

TA302803 (0.1µg/ml) staining of Human Duodenum lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

KLF4 antibody

Western blot analysis of KLF4 in human liver tissue lysate with KLF4 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

KLF4 antibody

KLF4 Antibody (C-term) (Cat. #TA324452) western blot analysis in A431,MCF-7 cell line lysates (35ug/lane).This demonstrates the KLF4 antibody detected the KLF4 protein (arrow). Rabbit IgG Purified Hu F, ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

KLF4 (N-term) antibody

KLF4 Antibody (N-term) (Cat. #TA324723) western blot analysis in Hela,MCF-7,SW480 cell line lysates (35ug/lane).This demonstrates the KLF4 antibody detected the KLF4 protein (arrow). Rabbit Ig Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

KLF4 antibody

Western blot analysis of KLF4 in mouse liver tissue lysate with KLF4 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide. Mouse IgG1 4G6E11 Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

KLF4 antibody

Western blot analysis of KLF4 in mouse liver tissue lysate with KLF4 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide. Mouse IgG1 4E5C3 Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

KLF4 antibody

Western Blot: KLF4 Antibody [TA337056] - Lysate from human 293 cells in the 1) absence and 2) presence of immunizing peptide, 3) mouse NIH 3T3 and 4) RAW cells probed with KLF4 antibody at 1 ug/ml (293) and 2 ug/ml (NIH 3T3 and RAW). I goat anti-rabbit Ig Rabbit IgG Purified Bov, Hu, Ms, Prim, Rt F, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

KLF4 antibody

Western blot analysis of extracts from CHO-K1 cells, transfected with pcDNA3.1-Hygro(+)-mKLF4-Flag construct (A) or transfected with pDNA3.1-Hygro(+)-Flag vector (B),using KLF4 mouse mAb (1:1000 diluted).Predicted band size:55/65KDa.Observed band size:55/65KDa. Mouse IgG2b 3B3-B11-E6 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

KLF4 antibody

Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and human rectum tissues (right) using KLF4 antibody with DAB staining. Mouse IgG1 1E5 Ascites Hu E, P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

KLF4 antibody

Immunohistochemical analysis of paraffin-embedded colon cancer tissues (left) and lung cancer tissues (right) using KLF4 antibody Cat.-No AM06517SU-N with DAB staining Mouse IgG1 1E6 Ascites Hu E, ICC/IF, P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

KLF4 antibody

Figure 2. Immunoflurescence analysis of KLF4 antibody AM11052PU-N (5 ug/ml). Hela cells transfected with pMX constructs of human KLF4 (top) and NIH3T3 cells transfected with pMX constructs of mouse KLF4 (bottom) were analyzed at approximately 62 hours after transfection. Mouse IgG1 56CT5.1.6 Purified Hu ICC/IF, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

KLF4 (80-129) antibody

Western blot of Transfected 293T cell lysate using KLF4 antibody Cat.-No AP07186PU-N Rabbit Purified Bt, Can, Eq, Hu, Mky, Prim P, WB
0.1 mg / €530.00
  Acris Antibodies GmbH

KLF4 Control Peptide

KLF4
50 µg / €160.00
  Acris Antibodies GmbH

KLF4 antibody

KLF4 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

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