KLF8 antibody

Principal name

KLF8 antibody

Alternative names for KLF8 antibody

KLF8, BKLF3, ZNF7, Krueppel-like factor 8, Basic krueppel-like factor 3, Zinc finger protein 741

SwissProt ID

O95600 (Human), Q8BLM0 (Mouse)

Gene ID

11279 (KLF8)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Por (Porcine), Can (Canine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Goat, Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of KLF8 antibody

KLF8 (Krueppel-like factor 8) is a protein that in humans is encoded by the KLF8 gene. It is also named as ZNF741, or BKLF3. Kruppel-like factor proteins, including KLF8, all contain a characteristic C-terminal DNA-binding domain containing 3 Kruppel-like zinc fingers, and they all recognize CACCC and GC boxes present in promoters and enhancers. The International Radiation Hybrid Mapping Consortium mapped the KLF8 gene to the X chromosome (WI-18614). Suske et al. (2005) stated that the human KLF8 gene maps to chromosome Xp11.21, and the mouse Klf8 gene to chromosome XF3. van Vliet et al. (2000) identified a cDNA encoding KLF8, also called ZNF741. Expression of KLF8 or only its zinc fingers showed that the fingers could bind to a CACCC box. Sequence analysis predicted that the 359-amino acid protein contains a nuclear localization signal upstream of the zinc fingers, a valine-rich hydrophobic stretch, and a pro-val-asp-leu-ser (PVDLS) motif. Yeast 2-hybrid and GST pull-down analysis demonstrated that CTBP2 interacts with KLF8 through the PVDLS motif.

General readings

van Vliet J, Turner J, Crossley M.
Human Kruppel-like factor 8: a CACCC-box binding protein that associates with CtBP and represses transcription.
Nucleic Acids Res. 2000 May 1;28(9):1955-62.
PMID: 10756197

19 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

KLF8 (N-term) antibody

TA302802 staining (2µg/ml) of Human Kidney lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

KLF8 antibody

WB Suggested Anti-KLF8 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Can, GP, Hu, Ms, Rb WB
50 µg / €325.00
  OriGene Technologies, Inc.

KLF8 antibody

WB Suggested Anti-KLF8 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Placenta Rabbit IgG Purified GP, Hu, Ms, Por, Rb WB
50 µg / €325.00
  OriGene Technologies, Inc.

KLF8 antibody

Immunofluorescence of purified MaxPab antibody to KLF8 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

KLF8 antibody

KLF8 MaxPab rabbit polyclonal antibody. Western Blot analysis of KLF8 expression in human colon. Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

KLF8 antibody

KLF8 MaxPab rabbit polyclonal antibody. Western Blot analysis of KLF8 expression in human colon. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

KLF8 antibody

Western Blot analysis of KLF8 expression in transfected 293T cell line by KLF8 monoclonal antibody (M09), clone 2E10.

Lane 1: KLF8 transfected lysate(39.3 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 2E10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

KLF8 antibody

Western Blot analysis of KLF8 expression in transfected 293T cell line by KLF8 monoclonal antibody (M12), clone 3F10.

Lane 1: KLF8 transfected lysate(39.3 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 3F10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

KLF8 antibody

KLF8 polyclonal antibody ( Cat # PAB6183 ) staining ( 2 µg/ml ) of human kidney lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

KLF8 antibody

Human kidney; KLF8 antibody - N-terminal region (ARP31533_P050) in Human kidney cells using Immunohistochemistry Rabbit Aff - Purified Hu P, WB
50 µg / €350.00
  AVIVA Systems Biology

KLF8 antibody

Human HepG2; Host: Rabbit. Target Name:KLF8. Sample Tissue: HepG2 Whole Cell lysates. Antibody Dilution: 1.0ug/ml.; KLF8 antibody - middle region (ARP32859_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

KLF8 antibody

Human Placenta; WB Suggested Anti-KLF8 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Placenta; KLF8 antibody - N-terminal region (ARP57952_P050) in Human Placenta cells using Western Blot Rabbit Aff - Purified Hu, Ms, Por, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

KLF8 antibody

KLF8 Rabbit IgG Aff - Purified Hu WB
0.1 mg / €290.00
  Boster Immunoleader

KLF8 antibody

NB 100-1050 staining (2µg/ml) of Human Kidney lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

KLF8 antibody

Immunohistochemistry: Immunohistochemical analysis of paraffin-embedded Meningioma, using KLF8 antibody at 1:100 dilution. Rabbit IgG Hu P
0.1 ml / €340.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

KLF8

KLF8 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

KLF8

KLF8 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Antibody Pairs

Catalog No.    

KLF8 (Human) IP-WB Antibody Pair

KLF8 (Human) IP-WB Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

KLF8 293T Cell Transient Overexpression Lysate(Denatured)

KLF8 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

19 Item(s)

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