KLK5 / Kallikrein-5 antibody

Principal name

KLK5 / Kallikrein-5 antibody

Alternative names for KLK5 / Kallikrein-5 antibody

Kallikrein-Like Protein 2, KLK-5, SCTE, KLK-L2, Stratum corneum tryptic enzyme

SwissProt ID

Q9Y337 (Human)

Gene ID

25818 (KLK5)

Available reactivities

Hu (Human), Can (Canine), Rt (Rat), Ms (Mouse), Bov (Bovine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Frozen Sections (C), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))

Background of KLK5 / Kallikrein-5 antibody

Kallikrein 5 was originally described from the skin as an enzyme involved in turnover of the stratum corneum, and later cloned from human EST libraries as a Kallikrein like enzyme. The skin is known to contain trypsin like and chymotrypsin like proteinases of 32 kDa and 25 kDa respectively, and these were determined to be hK5 and hK7. Kallikrein 5 is found is greatest abundance in the skin, and also in the brain, with lesser signal in the kidney, lung, mammary gland, and testis. Human breast milk is reported to contain high levels of Kallikrein 5. Less stringent PCR detects hK5 in many tissues, and analysis of cell lines by Western blot shows that many cell lines make hK5.

General readings

1. Michael IP, Pampalakis G, Mikolajczyk SD, Malm J, Sotiropoulou G, Diamandis EP.
Human tissue kallikrein 5 is a member of a proteolytic cascade pathway involved in seminal clot liquefaction and potentially in prostate cancer progression. J Biol Chem. 2006 May 5;281(18):12743-50. Epub 2006 Mar 3.
PMID: 16517595

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

KLK5 / Kallikrein-5 antibody

TA303202 (0.1µg/ml) staining of Human Prostate lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

KLK5 / Kallikrein-5 antibody

Western Blot: KLK L2 Antibody - Western blot of KLK5 (also known as KLK-L2) in BT-474 cells using anti-KLK5 at a 1:1,000 dilution Rabbit IgG Purified Hu C, E, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

KLK5 / Kallikrein-5 antibody

Predicted band size: 32 kDa . Positive control: Mouse brain tissue lysate. Recommended dilution: 1/1000-5000. (Gel: %SDS-PAGE Lysate: 40 ug Primary antibody: 1/1600 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

KLK5 / Kallikrein-5 antibody

WB Suggested Anti-KLK5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: THP-1 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

KLK5 / Kallikrein-5 antibody

Immunohistochemical staining of human skin shows strong positivity in the keratinized layer.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

KLK5 / Kallikrein-5 antibody

Western blot analysis of anti-KLK5 Pab in mouse brain tissue lysate.KLK5(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

KLK5 / Kallikrein-5 antibody

Figure 1. Western Blot of 10 µl conditional medium reduced by beta-Mercaptoethanol using KLK5 antibody (1/1000). The antibody is conjugated to Alkaline Phosphatase. Rabbit Serum Hu WB
0.2 ml / €390.00
  Acris Antibodies GmbH

KLK5 / Kallikrein-5 (pro-) antibody

Figure 1. Western Blot of 10 µl conditional medium  using pro-KLK5 antibody (1/1000).
Lane 1: Debatured Protein.
Lane 2: Native Protein.
The antibody is conjugated to Alkaline Phosphatase. Rabbit Serum Hu WB
0.2 ml / €390.00
  Acris Antibodies GmbH

KLK5 / Kallikrein-5 antibody

KLK5 / Kallikrein-5 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

KLK5 / Kallikrein-5 antibody

KLK5 / Kallikrein-5 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

KLK5 / Kallikrein-5 antibody

KLK5 / Kallikrein-5 Mouse IgG2a 3H3 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

KLK5 / Kallikrein-5 antibody

Western blot of 10 µl conditional medium reduced by beta- mercaptoethanol. KLK5 polyclonal antibody ( Cat # PAB0138 ) used 1 : 1000. The anti-rabbit antibody is conjugated to alkaline phosphatase. Rabbit IgG Purified Hu WB
0.2 ml / €640.00
  Abnova Taiwan Corp.

KLK5 / Kallikrein-5 pro-Kallikrein-5 antibody

Western blot of 10 µl conditional medium using rPro KLK5 polyclonal antibody ( Cat # PAB0139 ) 1 : 1000. 
1) Denatured protein. 
2) Native protein. 
The anti-rabbit antibody is conjugated to alkaline phosphatase. Rabbit IgG Purified Hu WB
0.2 ml / €640.00
  Abnova Taiwan Corp.

KLK5 / Kallikrein-5 antibody

Western blot analysis of KLK5 polyclonal antibody ( Cat # PAB4411 ) in mouse brain tissue lysate . KLK5 ( arrow ) was detected using the purified KLK5 polyclonal antibody ( Cat # PAB4411 ) . Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

KLK5 / Kallikrein-5 antibody

KLK5 polyclonal antibody ( Cat # PAB7227 ) ( 0.1 µg/ml ) staining of human prostate lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

KLK5 / Kallikrein-5 antibody

KLK5 / Kallikrein-5 Rabbit Serum Hu WB
0.2 ml / €390.00
  AgriSera AB

KLK5 / Kallikrein-5 antibody

KLK5 / Kallikrein-5 Rabbit Serum Hu WB
0.2 ml / €390.00
  AgriSera AB

KLK5 / Kallikrein-5 antibody

Human THP-1; WB Suggested Anti-KLK5 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: THP-1 cell lysate; KLK5 antibody - N-terminal region (ARP45010_P050) in Human THP-1 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

KLK5 / Kallikrein-5 antibody

Stimulation of ERK1/2 phosphorylation by NDMC in NG108-15 cells. (a) Immunofluorescence analysis of phospho-ERK1/2 immunoreactivity. Cells were serum-starved for 12 h and then treated with either vehicle (control), 10 M NDMC, 1 M NTI, and NTI+NDMC for 20 min, fixed and immunostained with anti-phospho-ERK1/2 antibody followed by FITC-conjugated secondary antibody. Results are representative of three similar experiments. (b) Western blot analysis of phospho-ERK1/2 immunoreactivity. Serum-starved cells were treated for 20 min with vehicle (lane 1), 10 M NDMC (lane 2), 1 M NTI (lane 3), and NTI+NDMC (lane 4). Thereafter, cell extracts were prepared and equal amounts of proteins (30 g) were loaded in each lane. Samples were subjected to immunoblotting with either anti-phospho-ERK1/2 antibody (top) or anti-ERK1 antibody (bottom). Results are representative of three similar experiments. (c) Densitometric analysis of immunoreactive phospho-ERK1/2 bands. The optical density of the phospho-ERK1/2 bands for each drug treatment was normalized to the density of the corresponding ERK1 band and is reported as percent of control. Values are the meanSEM of three experiments. *p0.05 vs control by one-way ANOVA followed by Dunnett's test. Mouse IgG2b 193318 Aff - Purified Hu E, WB
0.5 mg / €410.00
  Neuromics Antibodies

KLK5 / Kallikrein-5 antibody

KLK5 / Kallikrein-5 Rabbit Aff - Purified Hu WB
0.1 mg / €330.00
  Neuromics Antibodies

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