KPNA1 / Importin alpha-1 antibody

Principal name

KPNA1 / Importin alpha-1 antibody

Alternative names for KPNA1 / Importin alpha-1 antibody

Karyopherin alpha 1, SRP1, SRP1-beta, RCH2, IPOA5, NPI-1, Nucleoprotein interactor 1, RAG cohort protein 2

SwissProt ID

A2VE08 (Bovin), O14063 (Schpo), P52170 (Xenla), P52294 (Human), P83953 (Rat), Q02821 (Yeast), Q22560 (Caeel), Q5ZML1 (Chick), Q60960 (Mouse), Q96321 (Arath)

Gene ID

3836 (KPNA1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Chk (Chicken), Ze (Zebrafish), African clawed frog, Eq (Equine), Rb (Rabbit)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of KPNA1 / Importin alpha-1 antibody

Protein transfer into and out of the nucleus occurs through the nuclear pore complex (NPCs). Karyopherin, a cytosolic, heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex. SRP1 (nucleoprotein interactor 1, NPI 1) is the alpha subunit of
karyopherin, which acts as the receptor for the targeted protein’s nuclear localization signal (Moroianu J. Crit 1997). Both subunits of karyopherin are required for protein docking at the NPC; the alpha subunit binds the substrate and the beta subunit acts as an adaptor (Moroianu J, et al. 1995).
Two other proteins, p10 and the GTPase Ran, are also required for the translocation of docked proteins to the nucleus. The karyopherin dimer dissociates during import, a process mediated by RanGTP, which binds to karyopherin ß and promotes the release of both subunits from the docking site (Moroianu J. Crit 1997). The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC (Moroianu J. Crit 1997)

General readings

Cortes P, Ye ZS, Baltimore D. RAG-1 interacts with the repeated amino acid motif of the human homologue of the yeast protein SRP1. Proc Natl Acad Sci U S A. 1994 Aug 2;91(16):7633-7. PMID: 8052633

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

KPNA1 / Importin alpha-1 antibody

Western blot analysis of extracts of various cell lines, using KPNA1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

KPNA1 / Importin alpha-1 antibody

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 2A4-1B5 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

KPNA1 / Importin alpha-1 (N-term) antibody

TA302916 staining (1µg/ml) of HeLa lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

KPNA1 / Importin alpha-1 (N-term) antibody

Western blot analysis of KPNA1 in Hela cell lysate with KPNA1 antibody at 1ug/mL. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

KPNA1 / Importin alpha-1 antibody

Predicted band size: 60 kDa . Positive control: K562 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

KPNA1 / Importin alpha-1 antibody

Host: Rabbit; Target Name: KPNA1; Sample Tissue: Human Fetal Heart; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

KPNA1 / Importin alpha-1 antibody

WB Suggested Anti-KPNA1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

KPNA1 / Importin alpha-1 control peptide

KPNA1 / Importin alpha-1
50 µg / €160.00
  Acris Antibodies GmbH

KPNA1 / Importin alpha-1 antibody

KPNA1 polyclonal antibody (A02), Lot # 061025JCS1 Western Blot analysis of KPNA1 expression in NIH/3T3 ( Cat # L018V1 ). Mouse Serum Hu, Ms, Rt E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

KPNA1 / Importin alpha-1 antibody

Immunofluorescence of purified MaxPab antibody to KPNA1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

KPNA1 / Importin alpha-1 antibody

Immunofluorescence of purified MaxPab antibody to KPNA1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

KPNA1 / Importin alpha-1 antibody

KPNA1 MaxPab rabbit polyclonal antibody. Western Blot analysis of KPNA1 expression in mouse intestine. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

KPNA1 / Importin alpha-1 antibody

Immunoperoxidase of monoclonal antibody to KPNA1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1 ug/ml] Mouse IgG1 2A4-1B5 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

KPNA1 / Importin alpha-1 antibody

KPNA1 polyclonal antibody ( Cat # PAB6557 ) staining ( 1 µg/ml ) of HeLa lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

KPNA1 / Importin alpha-1 antibody

Hum. Fetal Heart; Host: Rabbit . Target Name: KPNA1 . Sample Tissue: Human Fetal Heart . Antibody Dilution: 1.0ug/ml.; KPNA1 antibody - N-terminal region (ARP54670_P050) in Hum. Fetal Heart cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Chk, Hu, Ms, Por, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

KPNA1 / Importin alpha-1 (N-term) antibody

Human HepG2; WB Suggested Anti-KPNA1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; KPNA1 antibody - N-terminal region (ARP54671_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Bov, Chk, Hu, Ms, Por, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

KPNA1 / Importin alpha-1 (transcript variant 1)

KPNA1 / Importin alpha-1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

KPNA1 / Importin alpha-1

KPNA1 / Importin alpha-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

KPNA1 / Importin alpha-1

KPNA1 / Importin alpha-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

KPNA1 293T Cell Transient Overexpression Lysate(Denatured)

KPNA1 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

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