KRAS antibody

Principal name

KRAS antibody

Alternative names for KRAS antibody

GTPase KRas, KRAS2, RASK2, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras

SwissProt ID

P01116 (Human), P08644 (Rat), P32883 (Mouse), Q05147 (Xenla)

Gene ID

3845 (KRAS), 16653 (Kras)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Flow Cytometry (F)

Background of KRAS antibody

K-ras, V-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog, is a protein that in humans is encoded by the KRAS gene. Like other members of the Ras family, the K-ras protein is a GTPase and is an early player in many signal transduction pathways. K-ras is usually tethered to cell membranes because of the presence of an isoprenyl group on its C-terminus. The K-ras gene spans 38 kb and contains 4 exons. By in situ hybridization, the K-ras gene is mapped to chromosome 12p12.1-p11.1. K-ras acts as a molecular on/off switch. Once it is turned on it recruits and activates proteins necessary for the propagation of growth factor and other receptors' signal, such as c-Raf and PI 3-kinase. K-ras binds to GTP in the active state and possesses an intrinsic enzymatic activity which cleaves the terminal phosphate of the nucleotide converting it to GDP

General readings

McGrath JP., et al. (1983) Nature. 304(5926): 501-506.Schubbert S., et al. (2006) Nat Genet. 38(3): 331-336.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

KRAS antibody

  KRAS mouse monoclonal antibody, clone OTI4F6 (formerly 4F6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRAS (RC222697, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRAS. Mouse IgG1 OTI4F6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

KRAS antibody

  KRAS mouse monoclonal antibody, clone OTI2C1 (formerly 2C1)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRAS (RC222697, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRAS. Mouse IgG2b OTI2C1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

KRAS antibody

  Anti-KRAS Rabbit Polyclonal Antibody  
Predicted band size: 21 kDa . Positive control: Brain tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

KRAS antibody

  KRAS mouse monoclonal antibody, clone OTI1D1 (formerly 1D1)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRAS (RC222697, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRAS. Mouse IgG1 OTI1D1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

KRAS antibody

  KRAS mouse monoclonal antibody, clone OTI3B12 (formerly 3B12)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRAS (RC222697, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRAS. Mouse IgG1 OTI3B12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

KRAS antibody

  KRAS mouse monoclonal antibody, clone OTI5H6 (formerly 5H6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRAS (RC222697, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRAS. Mouse IgG1 OTI5H6 Purified Hu WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

KRAS antibody

  KRAS mouse monoclonal antibody, clone OTI4C6 (formerly 4C6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRAS (RC222697, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRAS. Mouse IgG1 OTI4C6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

KRAS antibody

  KRAS mouse monoclonal antibody, clone OTI2F8 (formerly 2F8)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRAS (RC222697, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRAS. Mouse IgG1 OTI2F8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

KRAS antibody

  KRAS mouse monoclonal antibody, clone OTI4B3 (formerly 4B3)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRAS (RC222697, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRAS. Mouse IgG1 OTI4B3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

KRAS antibody

  KRAS mouse monoclonal antibody, clone OTI8B4 (formerly 8B4)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRAS (RC222697, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRAS. Mouse IgG2b OTI8B4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

KRAS antibody

  KRAS mouse monoclonal antibody, clone OTI6A6 (formerly 6A6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRAS (RC222697, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRAS. Mouse IgG1 OTI6A6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

KRAS antibody

  KRAS mouse monoclonal antibody, clone OTI2G6 (formerly 2G6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRAS (RC201958, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRAS. Mouse IgG1 OTI2G6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

KRAS antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRAS antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : HeLa cell lysate 
Lane 2. : HepG2 cell lysate 
Lane 3. : Ramos cell lysate 
Lane 4. : A549 cell lysate 
Lane 5. : Balb/3T3 cell lysate Mouse AT2F8 Purified Hu E, F, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

KRAS antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRAS antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : HeLa cell lysate 
Lane 2. : HepG2 cell lysate 
Lane 3. : Ramos cell lysate 
Lane 4. : A549 cell lysate 
Lane 5. : Balb/3T3 cell lysate Mouse AT2F8 Purified Hu E, F, ICC/IF, WB
50 µl / €220.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

KRAS - human gene knockout kit via CRISPR

KRAS - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

KRAS - human gene knockout kit via CRISPR

KRAS - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

KRAS - human gene knockout kit via CRISPR

KRAS - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

KRAS - human gene knockout kit via CRISPR

KRAS - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Kras - mouse gene knockout kit via CRISPR

Kras - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Kras - mouse gene knockout kit via CRISPR

Kras - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

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