Krueppel-like factor 1 (KLF1) antibody

Principal name

Krueppel-like factor 1 (KLF1) antibody

Alternative names for Krueppel-like factor 1 (KLF1) antibody

KLF1, EKLF, Krueppel-like factor 1, Erythroid transcription factor, KLF-1, Erythroid krueppel-like transcription factor

SwissProt ID

P46099 (Mouse), Q13351 (Human)

Gene ID

10661 (KLF1), 16596 (Klf1)

Available reactivities

Hu (Human), Bov (Bovine), Por (Porcine), Ms (Mouse), Rt (Rat), Ze (Zebrafish), Can (Canine), GP (Guinea Pig)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ChIP assay (ChIP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Krueppel-like factor 1 (KLF1) antibody

This product is one of a range of Investigative Grade antibodies, made against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues available to us. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot. However no additional claims are made for their ability to recognise native protein in any application. EKLF is an essential transcriptional activator that directs high-level expression of the adult beta-globin promoter by binding to its CACCC element. Probably serves as a general switch factor for erythroid development. Human EKLF is expressed in bone marrow and erythroleukemic cells lines but not in myeloid or lymphoid cell lines

General readings

Pandya K, Townes TM.Basic residues within the Kruppel zinc finger DNA binding domains are the critical nuclear localization determinants of EKLF/KLF-1.J Biol Chem. 2002 May 3;277(18):16304-12. PMID: 11844803

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

KLF1/5/7 antibody

Immunofluorescent analysis of KLF1/5/7 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu, Ms, Rt, Ze ICC/IF, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

KLF1/5/7 antibody

Immunofluorescent analysis of KLF1/5/7 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu, Ms, Rt, Ze ICC/IF, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

KLF1/5/7 antibody

Immunofluorescent analysis of KLF1/5/7 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu, Ms, Rt, Ze ICC/IF, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Krueppel-like factor 1 (KLF1) (N-term) antibody

Krueppel-like factor 1 (KLF1) Goat Aff - Purified Bov, Can, Hu, Ms, Por ChIP
0.1 mg / €325.00
  OriGene Technologies, Inc.

Krueppel-like factor 1 (KLF1) antibody

WB Suggested Anti-KLF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human kidney Rabbit IgG Purified Bov, Can, GP, Hu, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Krueppel-like factor 1 (KLF1) antibody

WB Suggested Anti-KLF1 Antibody Titration: 2.5ug/ml; Positive Control: K562 cell lysateKLF1 is supported by BioGPS gene expression data to be expressed in K562 Rabbit IgG Purified Bov, Hu, Por WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Krueppel-like factor 1 (KLF1) (+GKLF) antibody

Western blot (WB) analysis of EKLF/GKLF antibody (Cat.-No.: AP20438PU-N) in extracts from Jurkat cell treated with serum 20% 15' Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Krueppel-like factor 1 (KLF1) antibody

Krueppel-like factor 1 (KLF1) Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Krueppel-like factor 1 (KLF1) antibody

Detection limit for recombinant GST tagged KLF1 is approximately 0.03ng/ml as a capture antibody. Mouse IgG2a 5D7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Krueppel-like factor 1 (KLF1) antibody

KLF1 monoclonal antibody (M02), clone 4E10 Western Blot analysis of KLF1 expression in K-562 ( Cat # L009V1 ). Mouse IgG3 4E10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Krueppel-like factor 1 (KLF1) antibody

KLF1 monoclonal antibody (M03), clone 1E4 Western Blot analysis of KLF1 expression in K-562 ( Cat # L009V1 ). Mouse IgG3 1E4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Krueppel-like factor 1 (KLF1) antibody

Immunofluorescence of monoclonal antibody to KLF1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG3 5G12 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Krueppel-like factor 1 (KLF1) antibody

KLF1 monoclonal antibody (M05), clone 2A6 Western Blot analysis of KLF1 expression in K-562 ( Cat # L009V1 ). Mouse IgG3 2A6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Krueppel-like factor 1 (KLF1) antibody

Krueppel-like factor 1 (KLF1) Rabbit Ms E, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Krueppel-like factor 1 (KLF1) antibody

Krueppel-like factor 1 (KLF1) Goat Hu, Ms E
0.1 mg / €400.00
  Abnova Taiwan Corp.

Krueppel-like factor 1 (KLF1) antibody

Western blot analysis of extracts from Jurkat cells, treated with serum 20% 15', using KLF Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

Krueppel-like factor 1 (KLF1) antibody

Western blot analysis of extracts from Jurkat cells, treated with serum 20% 15', using KLF Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

Krueppel-like factor 1 (KLF1) antibody

Human Jurkat; WB Suggested Anti-KLF1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Jurkat cell lysate; KLF1 antibody - middle region (ARP31493_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Bov, Hu, Ms, Por, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

Krueppel-like factor 1 (KLF1) antibody

Human kidney; WB Suggested Anti-KLF1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human kidney; KLF1 antibody - N-terminal region (ARP32809_P050) in Human kidney cells using Western Blot Rabbit Aff - Purified Bov, Hu, Por WB
50 µg / €350.00
  AVIVA Systems Biology

Krueppel-like factor 1 (KLF1) antibody

Human K562; WB Suggested Anti-KLF1 Antibody Titration: 2.5ug/ml. Positive Control: K562 cell lysate. KLF1 is supported by BioGPS gene expression data to be expressed in K562.; KLF1 antibody - middle region (ARP32810_T100) in Human K562 cells using Western Blot Rabbit Aff - Purified Bov, Hu, Por WB
0.1 mg / €310.00
  AVIVA Systems Biology

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