lacI antibody

Principal name

lacI antibody

Alternative names for lacI antibody

Lactose operon repressor, b0345, JW0336

SwissProt ID

P03023 (Ecoli), P06220 (Klepn), Q01936 (Rhird)

Gene ID

945007 (lacI)

Available reactivities

Hu (Human), E. coli (Escherichia coli), Chk (Chicken)

Available hosts

Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB)

Background of lacI antibody

The lac repressor protein (Lac I) is an important genetic control protein. It controls the expression of the lactose metabolizing enzymes in Escherichia coli. This regulation involves specific recognition of the operator DNA sequence (Lac O) and consequent inhibition of transcription initiation by RNA polymerase; modulation of binding at this target site by small sugar molecules, inducers, provides the basis for regulation. Different ligand binding activities are associated with the isolated domains of Lac I: specific and nonspecific DNA binding with the NH2 termini, and inducer and specific DNA binding with the core protein. This antibody could possibly be used to recover and purify protein/nucleic acid complexes (host and viral proteins associated with the viral DNA) from infected cells.

3 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

lacI antibody

Western blot using Affinity purified anti-Lac I antibody shows detection of a 38 kDa band corresponding to recombinant Lac I (arrowhead). The blot was blocked with 5% BLOTTO in TTBS overnight at 4°C. Primary antibody was used at a 1/1,000 dilution followed by reaction with a 1/10,000 dilution of HRP Goat anti-Rabbit IgG. ECL was used for detection. Rabbit IgG Aff - Purified E. coli, Shigella Sonnei E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

lacI antibody

Western blot using Affinity purified anti-Lac I antibody shows detection of a 38 kDa band corresponding to recombinant Lac I (arrowhead). The blot was blocked with 5% BLOTTO in TTBS overnight at 4°C. Primary antibody was used at a 1/1,000 dilution followed by reaction with a 1/10,000 dilution of HRP Goat anti-Rabbit IgG. ECL was used for detection. Rabbit IgG Aff - Purified E. coli, Shigella Sonnei E, WB
25 µl / €190.00
  Acris Antibodies GmbH

lacI antibody

Western blot using lacI polyclonal antibody ( Cat # PAB10255 ) shows detection of a 38 kDa band corresponding to recombinant lacI ( arrowhead ). The blot was blocked with 5% BLOTTO in TTBS overnight at 4°C. Primary antibody was used at a 1 : 1,000 dilution followed by reaction with a 1 : 10,000 dilution of HRP goat anti-rabbit IgG. ECL was used for detection.
Personal communication, S. Hughes & M. Abram, NCI, Bethesda, MD. Rabbit Hu E, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

3 Item(s)

per page
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