Lactadherin antibody

Principal name

Lactadherin antibody

Alternative names for Lactadherin antibody

MFGE8, Milk fat globule-EGF factor 8, HMFG, Breast epithelial antigen BA46, MFGM

SwissProt ID

P21956 (Mouse), P70490 (Rat), P79385 (Pig), Q08431 (Human), Q95114 (Bovin)

Gene ID

4240 (MFGE8), 17304 (Mfge8)

Available reactivities

Hu (Human), Ms (Mouse)

Available hosts

Mouse, Rabbit, Hamster

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Flow Cytometry (F)

Background of Lactadherin antibody

MFGE8 (Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. Mfge8 is secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, Collins et al. (1997) mapped the MFGE8 gene to chromosome 15q25. Hanayama et al. (2002) found that MFGE8 is a factor that links apoptotic cells to phagocytes. MFGE8 specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. Bu et al. (2007) showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon (PRKCE)-dependent mechanism.

General readings

Yamaguchi, H., et al. Eur. J. Immunol. 40(6):1778-1785(2010)
Wheeler, H.E., et al. PLoS Genet. 5 (10), E1000685 (2009)
Hu, C.Y., et al. Lupus 18(8):676-681(2009)
Fu, Z., et al. Circ. Res. 104(12):1337-1346(2009)
Raymond, A., et al. J. Cell. Biochem. 106(6):957-966(2009)

Items 1 to 20 of 33 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Lactadherin antibody

  Lactadherin / MFGE8 Mouse Monoclonal (B656M) Antibody  
Anti-MFGE8 / MFG-E8 antibody IHC of human placenta, trophoblast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. Mouse IgG1 B656M Purified Hu C, ICC/IF, WB
50 µg / €345.00
  OriGene Technologies, Inc.

Lactadherin antibody

Flow cytometric analysis of MFG-E8 binding to apoptotic Jurkat cells. Apoptotic Jurkat cells induced by anti-Fas (CH-11/code no. SY001) were incubated with recombinat MFG-E8. MFG-E8 bound to the apoptotic cells was detected with AM26495AF-N. Open histogram indicates the reaction of isotypic control to the cells. Shaded histogram indicates the reaction of AM26495AF-N to the cells. Hamster IgG 2422 Azide Free Ms F, IP
0.1 mg / €370.00
  Acris Antibodies GmbH

Lactadherin antibody

Western blot analysis of MFG-E8 expression on mouse epididymides (1), recombinant HPC4 tagged mouse MFG-E8 short form (2) and long form (3) using AM26517AF-N. Hamster IgG 18A2-G10 Azide Free Ms C, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

Lactadherin antibody

Human Lung, Epithelium: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 1E7 Purified Hu E, IP, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

Lactadherin (C-term) antibody

Western blot analysis of  Lactadherin Antibody (C-term) Cat.-No AP52057PU-N in Hela cell line lysates (35ug/lane). Rabbit Ig Aff - Purified Hu F, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Lactadherin antibody

Lactadherin Mouse IgG1 MFG-06 Purified C, E, P, WB
0.2 mg / €400.00
  Acris Antibodies GmbH

Lactadherin antibody

Lactadherin Mouse IgG1 MFG-06 Purified Hu C, E, ICC/IF, P, WB
0.1 mg / €190.00
  Acris Antibodies GmbH

Lactadherin antibody

Lactadherin Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Lactadherin antibody

MFGE8 MaxPab polyclonal antibody. Western Blot analysis of MFGE8 expression in human pancreas. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Lactadherin antibody

Immunoprecipitation of MFGE8 transfected lysate using anti-MFGE8 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with MFGE8 purified MaxPab mouse polyclonal antibody (B01P) (H00004240-B01P). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

Lactadherin antibody

Western Blot analysis of MFGE8 expression in transfected 293T cell line (H00004240-T02) by MFGE8 MaxPab polyclonal antibody.

Lane 1: MFGE8 transfected lysate(43.10 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Lactadherin antibody

Detection limit for recombinant GST tagged MFGE8 is approximately 0.3ng/ml as a capture antibody. Mouse IgG2a 1E7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Lactadherin antibody

Detection limit for recombinant GST tagged MFGE8 is approximately 0.3ng/ml as a capture antibody. Mouse IgG2a 3E9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Lactadherin antibody

Lactadherin Mouse IgG1 MFG-06 Hu P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Lactadherin antibody

Lactadherin Rabbit IgG Aff - Purified Hu P, WB
0.1 mg / €290.00
  Boster Immunoleader

CRISPR

Catalog No. Species Donor Vector  

MFGE8 - human gene knockout kit via CRISPR

MFGE8 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

MFGE8 - human gene knockout kit via CRISPR

MFGE8 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

MFGE8 - human gene knockout kit via CRISPR

MFGE8 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

MFGE8 - human gene knockout kit via CRISPR

MFGE8 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Lactadherin (transcript variant 1)

  Recombinant protein of human milk fat globule-EGF factor 8 protein (MFGE8), transcript variant 1  
Lactadherin Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Items 1 to 20 of 33 total

per page
Page:
  1. 1
  2. 2
  • LinkedIn