LARS2 antibody
Principal name
LARS2 antibody
Alternative names for LARS2 antibody
LARS2, KIAA0028, Leucyl-tRNA synthetase, mitochondrial, EC=6.1.1.4, Leucine-tRNA ligase, LeuRS
SwissProt ID
P11325 (Yeast), P13503 (Sacdo), P15181 (Neucr), Q09828 (Schpo), Q15031 (Human), Q54ET5 (Dicdi), Q5RDP4 (Ponab), Q8VDC0 (Mouse)
Ncbi ID
9606, NP_056155, NM_015340, NP_056155.1, NP_694808.1, NM_153168
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of LARS2 antibody
- Kit Component:
- - KN309142G1, Lars2 gRNA vector 1 in pCas-Guide vector
- KN309142G2, Lars2 gRNA vector 2 in pCas-Guide vector
- KN309142D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Li, R., et al. Mol. Cell. Biol. 30(9):2147-2154(2010)
Reiling, E., et al. Diabetologia 53(1):103-110(2010)
Zhou, W., et al. Acta Biochim. Biophys. Sin. (Shanghai) 41(1):54-62(2009)
Park, H., et al. RNA 14(11):2407-2416(2008)
't Hart, L.M., et al. Diabetes 54(6):1892-1895(2005)
Western Blot analysis of LARS2 expression in transfected 293T cell line (H00023395-T03) by LARS2 MaxPab polyclonal antibody.Lane 1: LARS2 transfected lysate(102.00 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of LARS2 Antibody (Center) Cat.-No AP52445PU-N in CEM cell line lysates (35ug/lane). This demonstrates the LARS2 antibody detected the LARS2 protein (arrow).
Flow cytometric analysis of K562 cells using LARS2 Antibody (Center) Cat.-No AP52445PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Sample (30 ug of whole cell lysate). A: Raji. 7.5% SDS PAGE. TA308130 diluted at 1:5000
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using LARS2(TA308130) antibody at 1:500 dilution.
LARS2 antibody [N1N2], N-term detects LARS2 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37â for 30 min. Green: LARS2 protein stained by LARS2 antibody [N1N2], N-term (TA308
Sample (30 ug of whole cell lysate). A: Molt-4 . 7.5% SDS PAGE. TA308628 diluted at 1:3000
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using LARS2(TA308628) antibody at 1:100 dilution.
Immunofluorescence analysis of methanol-fixed A431, using LARS2(TA308628) antibody at 1:200 dilution.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308130 | LARS2 antibody |
||||||
| Rabbit Polyclonal antibody to LARS2 (leucyl-tRNA synthetase 2, mitochondrial) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
|||||||
| TA308628 | LARS2 antibody |
||||||
| Rabbit Polyclonal antibody to LARS2 (leucyl-tRNA synthetase 2, mitochondrial) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| AP52445PU-N | LARS2 (Center) antibody |
||||||
 |
Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€370.00
|
|
| Acris Antibodies GmbH | |||||||
| +1 additional image |
|||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN205207 | LARS2 - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,200.00
|
| OriGene Technologies, Inc. | |||
| KN205207BN | LARS2 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN205207LP | LARS2 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN205207RB | LARS2 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN309142 | Lars2 - mouse gene knockout kit via CRISPR |
||
|
Mouse | GFP-Puro |
1 kit /
€1,200.00
|
| OriGene Technologies, Inc. | |||
| KN309142BN | Lars2 - mouse gene knockout kit via CRISPR |
||
|
Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN309142LP | Lars2 - mouse gene knockout kit via CRISPR |
||
|
Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN309142RB | Lars2 - mouse gene knockout kit via CRISPR |
||
|
Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Lysates
| Catalog No. | ||
|---|---|---|
| LY414602 | LARS2 overexpression lysate |
|
|
0.1 mg /
€280.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC205207L2V | Lenti ORF particles, LARS2 (mGFP-tagged) - Human leucyl-tRNA synthetase 2, mitochondrial (LARS2), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,050.00
|
|
| OriGene Technologies, Inc. | ||
| RC205207L1V | Lenti ORF particles, LARS2 (Myc-DDK tagged) - Human leucyl-tRNA synthetase 2, mitochondrial (LARS2), nuclear gene encoding mitochondrial protein , 200 uL, >10^7 TU/mL |
|
|
200 µl /
€940.00
|
|
| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR211108L2V | Lenti ORF particles, Lars2 (GFP-tagged) - Mouse leucyl-tRNA synthetase, mitochondrial (Lars2), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,650.00
|
|
| OriGene Technologies, Inc. | ||
| MR211108L1V | Lenti ORF particles, Lars2 (Myc-DDK-tagged) - Mouse leucyl-tRNA synthetase, mitochondrial (Lars2), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,480.00
|
|
| OriGene Technologies, Inc. | ||
