LASS2 antibody

Principal name

LASS2 antibody

Alternative names for LASS2 antibody

LASS2, TMSG1, LAG1 longevity assurance homolog 2, SP260, Tumor metastasis-suppressor gene 1 protein

SwissProt ID

Q3ZBF8 (Bovin), Q924Z4 (Mouse), Q96G23 (Human)

Gene ID

29956 (CERS2), 76893 (Lass2)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Paraffin Sections (P)

Background of LASS2 antibody

Kit Component:
- KN303182G1, Cers2 gRNA vector 1 in pCas-Guide vector
- KN303182G2, Cers2 gRNA vector 2 in pCas-Guide vector
- KN303182D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

1: Becker I, Wang-Eckhardt L, Yaghootfam A, Gieselmann V, Eckhardt M.Differential expression of (dihydro)ceramide synthases in mouse brain: oligodendrocyte-specific expression of CerS2/Lass2.
Histochem Cell Biol. 2008 Feb;129(2):233-41. Epub 2007 Sep 28.
2: Wang X, Carre W, Saxton AM, Cogburn LA. Manipulation of thyroid status and/or GH injection alters hepatic gene expression in the juvenile chicken. Cytogenet Genome Res. 2007;117(1-4):174-88.
3: Senkal CE, Ponnusamy S, Rossi MJ, Bialewski J, Sinha D, Jiang JC, Jazwinski SM, Hannun YA, Ogretmen B. Role of human longevity assurance gene 1 and C18-ceramide in chemotherapy-induced cell death in human head and neck squamous cell carcinomas. Mol Cancer Ther. 2007 Feb;6 (2):712-22.
4: Yu Y, Lu H, Pan H, Ma JH, Ding ZJ, Li YY. Expression of LASS2 controlled by LAG1 or ADH1 promoters cannot functionally complement Lag1p. Microbiol Res. 2006;161(3):203-11. Epub 2005 Sep 22.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

LASS2 antibody

  Anti-CERS2 Rabbit Polyclonal Antibody  
Predicted band size: 45 kDa. Positive control: Human liver cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/750 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 40 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

LASS2 antibody

  Anti-CERS2 Rabbit Polyclonal Antibody  
Predicted band size: 45 kDa. Positive control: Human liver cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/1000 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 40 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

LASS2 antibody

  Rabbit Polyclonal Anti-LASS2 Antibody  
WB Suggested Anti-LASS2 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysateCERS2 is supported by BioGPS gene expression data to be expressed in HepG2 Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

LASS2 antibody

  Rabbit Polyclonal Anti-LASS2 Antibody  
WB Suggested Anti-LASS2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human brain Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

LASS2 antibody

  Rabbit Polyclonal Anti-LASS2 Antibody  
LASS2 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

LASS2 Control Peptide

LASS2 Purified
50 µg / €220.00
  Acris Antibodies GmbH

LASS2 antibody

Western blot analysis using LAG1 longevity assurance homolog 2 (AP05284PU-N) at 10 µg/ml on human placenta lysate 14 µg/lane. Lane A] antibody alone, Lane B] conjugate alone. Visualized using Pierce West Femto substrate system. Anti Rabbit
secondary used at 1:10K dilution. Exposure for 5 minutes Rabbit Aff - Purified Hu WB
50 µg / €490.00
  Acris Antibodies GmbH

LASS2 antibody

Western blot analysis using LAG1 longevity assurance homolog 2 (AP05284SU-N) at 10ug/ml on human placenta lysate 14 µg/lane. Lane A] antibody alone, Lane B] conjugate alone. Visualized using Pierce West Femto substrate system. Anti Rabbit secondary used at 1:10K dilution. Exposure for 5 minutes Rabbit Purified Hu WB
0.1 mg / €380.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

CERS2 - human gene knockout kit via CRISPR

CERS2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

CERS2 - human gene knockout kit via CRISPR

CERS2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

CERS2 - human gene knockout kit via CRISPR

CERS2 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Cers2 - mouse gene knockout kit via CRISPR

Cers2 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Cers2 - mouse gene knockout kit via CRISPR

Cers2 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Cers2 - mouse gene knockout kit via CRISPR

Cers2 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

LASS2 (transcript variant 2)

  Purified recombinant protein of Homo sapiens LAG1 homolog, ceramide synthase 2 (LASS2), transcript variant 2  
LASS2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

LASS2 (transcript variant 1)

  Recombinant protein of human LAG1 homolog, ceramide synthase 2 (LASS2), transcript variant 1  
LASS2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

CERS2 overexpression lysate

CERS2 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

CERS2 overexpression lysate

CERS2 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

CERS2 overexpression lysate

CERS2 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, CERS2 (mGFP-tagged) - Human LAG1 homolog, ceramide synthase 2 (LASS2), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, CERS2 (mGFP-tagged) - Human LAG1 homolog, ceramide synthase 2 (LASS2), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €960.00
  OriGene Technologies, Inc.

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