LAT antibody

Principal name

LAT antibody

Alternative names for LAT antibody

pp36, p36-38, 36 kDa Phospho-Tyrosine Adapter Protein, Linker For Activation of T-Cells Family Member 1

SwissProt ID

O43561 (Human), O54957 (Mouse), O70601 (Rat)

Gene ID

27040 (LAT), 81511 (Lat), 16797

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Dot blot (Dot), Frozen Sections (C), Flow Cytometry (F)

Background of LAT antibody

This LAT.10-17 (10-17) monoclol antibody reacts with human Linker for Activation of T cells (LAT), which is essential for T cell receptor (TCR)-mediated sigl transduction. Characterized as an integral transmembrane protein, this ~37-kDa molecule does not possess a true extracellular domain. Upon TCR/CD3 engagement, LAT localizes to lipid rafts and is tyrosine phosphorylated by ZAP-70, leading to the recruitment and activation of other downstream sigling molecules such as Grb-2, PLCγ, and PI3K. This adapter molecule has been shown to be required for T cell activation, as well as thymocyte and T regulatory cell development. In humans, LAT is expressed on peripheral blood lymphocytes, lymph nodes, and tonsil. Studies in mice have shown that LAT is expressed in thymocytes, T cells, mast cells, tural killer cells, megakaryocytes, platelets, and early B cells. This antibody does not crossreact with the mouse antigen.

General readings

Finco TS et al. LAT is required for TCR-mediated activation of PLCgamma1 and the Ras pathway. Immunity 9:617-26 (1998). PubMed PMID: 9846483

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

LAT (C-term) antibody

TA302672 (0.01µg/ml) staining of peripheral blood mononucleocytes lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt E, WB
20 µg / €200.00
  Acris Antibodies GmbH

LAT (C-term) antibody

TA302672 (0.01µg/ml) staining of peripheral blood mononucleocytes lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

LAT antibody

Immunoblotting of 10e6 normal peripheral human blood cells (lane 1), Jurkat (lane 2), and C57BL/6 splenocytes (lane 3) with 5 ug/mL Anti-Human LAT Purified.  Bands were visualized using Anti-Mouse IgG HRP. Mouse LAT.10-17 (10-17) Purified Hu IP, WB
25 µg / €120.00
  OriGene Technologies, Inc.

LAT antibody

Western blot analysis of extracts from 293 cells, using LAT (Ab-255) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

LAT antibody

Western blot analysis of extracts from Jurkat cells (lane 1) and mouse thymocytes (lane 2) using anti-LAT, clone 11B.12. Mouse IgG1 11B.12 Purified Hu, Ms IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

LAT antibody

Western blot analysis of extracts of Jurkat cell line, using LAT antibody. Rabbit IgG Purified Hu
0.1 mg / €325.00
  OriGene Technologies, Inc.

LAT antibody

Figure 1B. Distribution of LAT and NTAL on rat basophilic leukemia cells. Mouse IgG2a LAT-1D1 Aff - Purified Ms, Rt ICC/IF, IP, WB
0.1 mg / €240.00
  Acris Antibodies GmbH

LAT antibody

IHC Image of AM20572PU-N
Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 LAT.10-17 (10-17) Aff - Purified Hu IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

LAT antibody

Western blot analysis of extracts from Jurkat cells (Lane 1) and Mouse thymocytes (Lane 2) using anti-LAT Antibody Clone 11B.12. Mouse IgG1 11B.12 Purified Hu, Ms IP, WB
25 µg / €220.00
  Acris Antibodies GmbH

LAT (Cytopl. Dom.) antibody

Whole cell extract from human peripheral blood mononuclear cells was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-LAT Antibody Cat.-No AM33107PU (Clone LAT1111). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system. Mouse IgG1 LAT1111 Aff - Purified Hu IP, P, WB
0.1 mg / €310.00
  Acris Antibodies GmbH

LAT (Cytopl. Dom.) antibody

Whole cell extract from human peripheral blood mononuclear cells was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-LAT Antibody Cat.-No AM33107PU (Clone LAT1111). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system. Mouse IgG1 LAT1111 Aff - Purified Hu IP, P, WB
25 µg / €210.00
  Acris Antibodies GmbH

LAT antibody

Western blot (WB) analysis of LAT antibody (Cat.-No.: AP06532PU-N) in extracts from A549 cells. Rabbit Aff - Purified Hu, Ms WB
0.1 mg / €350.00
  Acris Antibodies GmbH

LAT antibody

Western blot (WB) analysis of LAT antibody (Cat.-No.: AP06533PU-N) in extracts from NIH/3T3 cells treated with 293 UV 5'. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

LAT antibody

Western blot (WB) analysis of LAT antibody (Cat.-No.: AP21126PU-N) in extracts from Jurkat cells. Rabbit Aff - Purified Hu WB
0.1 mg / €350.00
  Acris Antibodies GmbH

LAT antibody

Flow Cytometry analysis of LAT expression in peripheral blood lymphocytes using anti-human LAT (LAT-01) (surface markers were stained prior to intracellular staining of human LAT). Panel A: gate on CD4+ cells. Panel B: gate on CD8+ cells. Mouse IgG1 LAT-01 Purified Hu F, IP, WB
0.1 mg / €240.00
  Acris Antibodies GmbH

LAT antibody

LAT Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

LAT antibody

Immunoperoxidase of monoclonal antibody to LAT on formalin-fixed paraffin-embedded human tonsil tissue. [antibody concentration 5ug/ml] Mouse IgG1 3B11-1D5 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

LAT antibody

Distribution of Lat and NTAL in separate clusters on mast cell plasma membrane sheets.
Lat and NTAL were detected on mouse bone marrow-derived mast cells ( BMMC ; A ) and RBL rat basophilic leukemia cell line ( B ) by Lat monoclonal antibody, clone LAT-1D1 ( Cat # MAB3623 ) and polyclonal anti-NTAL antibody, followed by 5 or 10 nm gold-conjugated secondary antibodies, respectively. Mouse IgG2a LAT-1D1 Ms, Rt ICC/IF, IP, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

LAT antibody

Flow Cytometry analysis of LAT expression in human peripheral blood lymphocytes ( PBL ) using LAT monoclonal antibody, clone LAT-01 ( Cat # MAB3865 ) (surface markers were stained prior to intracellular staining of human LAT).
Panel A : gate on CD4+ cells.
Panel B : gate on CD8+ cells. Mouse IgG1 LAT-01 Hu F, IP, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

LAT antibody

LAT polyclonal antibody ( Cat # PAB6652 ) ( 0.01 µg/ml ) staining of peripheral blood mononucleocytes lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu, Ms, Rt E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

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