LAT2 / WBSCR15 antibody

Principal name

LAT2 / WBSCR15 antibody

Alternative names for LAT2 / WBSCR15 antibody

LAB, NTAL, WBS15, WBSCR5, HSPC046, Linker for activation of T-cells family member 2, Non-T-cell activation linker, LLinker for activation of B-cells, Membrane-associated adapter molecule, Williams-Beuren syndrome chromosomal region 15 protein

SwissProt ID

Q8CGL2 (Rat), Q9GZY6 (Human), Q9JHL0 (Mouse)

Gene ID

7462 (LAT2), 56743 (Lat2), 317676

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Enzyme Immunoassay (E)

Background of LAT2 / WBSCR15 antibody

Kit Component:
- KN309146G1, Lat2 gRNA vector 1 in pCas-Guide vector
- KN309146G2, Lat2 gRNA vector 2 in pCas-Guide vector
- KN309146D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

1. Yamasaki S, Ishikawa E, Sakuma M, Kanagawa O, Cheng AM, Malissen B, Saito T.: LAT and NTAL mediate immunoglobulin E-induced sustained extracellular signal-regulated kinase activation critical for mast cell survival. Mol Cell Biol. 2007 Jun;27(12):4406-15.
2. Brdicka T, Imrich M, Angelisova P, Brdickova N, Horvath O, Spicka J, Hilgert I, Luskova P, Draber P, Novak P, Engels N, Wienands J, Simeoni L, Osterreicher J, Aguado E, Malissen
M, Schraven B, Horejsi V.: Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling. J Exp Med. 2002 Dec 16;196 12):1617-26.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

LAT2 / WBSCR15 antibody

Subcellular localization of NTAL by confocal microscopy in THP-1 human acute monocytic leukemia cell line. THP-1 cells were permeabilized and immunostained using anti-NTAL (NAP-07; green). Nuclei are vizualized by propidium iodide (red). Mouse IgG1 NAP-07 Purified Hu, Ms F, ICC/IF, IP, WB
0.1 mg / €260.00
  Acris Antibodies GmbH

LAT2 / WBSCR15 antibody

Staining of Human Peripheral Lymphocytes lysate using LAT2 Antibody Cat.-No AP32820PU-N (0.01 µg/ml) (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

LAT2 / WBSCR15 antibody

  LAT2 / NTAL Mouse Monoclonal (aa196-212) (NAP-03) Antibody  
Anti-LAT2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. Mouse IgG2a P-03 Purified Hu
50 µg / €400.00
  OriGene Technologies, Inc.

LAT2 / WBSCR15 antibody

  Rabbit polyclonal anti-NATL/LAB antibody  
Western blot analysis of NTAL expression in rat kidney tissue lysate. Rabbit Purified Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

LAT2 / WBSCR15 antibody

  Anti-LAT2 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human colon cancer tissue. Recommended dilution: 1/10-50  The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using LAT2 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

LAT2 / WBSCR15 antibody

Immunohistochemistry staining of human spleen (paraffin sections) with anti-NTAL polyclonal. Rabbit Aff - Purified Hu P, WB
0.1 mg / €260.00
  Acris Antibodies GmbH

LAT2 / WBSCR15 antibody

Figure 1. Western Blotting analysis (non-reducing conditions) of mouse NTAL in mouse spleen tissue:  Lane 1: immunostaining with polyclonal anti-NTAL antibody (AP03007PU-N); Lane 2: immunostaining with polyclonal anti-mouse NTAL antibody (specific for N-terminal domain of mouse NTAL). Rabbit Aff - Purified Ms WB
0.1 mg / €260.00
  Acris Antibodies GmbH

LAT2 / WBSCR15 antibody

Distribution of LAT and NTAL in separate clusters on mast cell plasma membrane sheets. LAT and NTAL were detected on mouse bone marrow-derived mast cells (BMMC; 2A) and RBL rat basophilic leukemia cell line (2B) by anti-LAT (LAT-1D1; AM03086PU-N) and polyclonal anti-NTAL antibody, followed by 5 or 10 nm gold-conjugated secondary antibodies, respectively. Rabbit Aff - Purified Ms, Rt ICC/IF, WB
0.1 mg / €260.00
  Acris Antibodies GmbH

LAT2 / WBSCR15 (91-243) antibody

Spleen, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

LAT2 / WBSCR15 (Center) antibody

Western blot analysis in Hela cell line lysates (35ug/lane) using LAT2 / WBSCR15 antibody Cat.-No. AP52448PU-N. This demonstrates the LAT2 antibody detected the LAT2 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

LAT2 / WBSCR15 antibody

Subcellular localization of NTAL by confocal microscopy in THP-1 human acute monocytic leukemia cell line.
THP-1 cells were permeabilized and immunostained using anti-NTAL (NAP-07; green). Nuclei are vizualized by propidium iodide (red). Mouse IgG1 NAP-07 PE Hu, Ms F
0.1 mg / €410.00
  Acris Antibodies GmbH

LAT2 / WBSCR15 antibody

LAT2 / WBSCR15 Mouse IgG1 NAP-03 Purified Hu IP, P, WB
0.1 mg / €260.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

LAT2 - human gene knockout kit via CRISPR

LAT2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

LAT2 - human gene knockout kit via CRISPR

LAT2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

LAT2 - human gene knockout kit via CRISPR

LAT2 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

LAT2 - human gene knockout kit via CRISPR

LAT2 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lat2 - mouse gene knockout kit via CRISPR

Lat2 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Lat2 - mouse gene knockout kit via CRISPR

Lat2 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lat2 - mouse gene knockout kit via CRISPR

Lat2 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lat2 - mouse gene knockout kit via CRISPR

Lat2 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

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