LAT2 / WBSCR15 antibody

Principal name

LAT2 / WBSCR15 antibody

Alternative names for LAT2 / WBSCR15 antibody

LAB, NTAL, WBS15, WBSCR5, HSPC046, Linker for activation of T-cells family member 2, Non-T-cell activation linker, LLinker for activation of B-cells, Membrane-associated adapter molecule, Williams-Beuren syndrome chromosomal region 15 protein

SwissProt ID

Q8CGL2 (Rat), Q9GZY6 (Human), Q9JHL0 (Mouse)

Gene ID

7462 (LAT2), 56743 (Lat2), 317676, 841869 (AT1G54280)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Enzyme Immunoassay (E), Dot blot (Dot)

Background of LAT2 / WBSCR15 antibody

The adaptor protein NTAL (Non-T cell Activation Linker; human 30 kDa, mouse 25 kDa), alternatively termed as linker for activation of B cells (LAB), is expressed in plasma membrane lipid rafts of B cells, mast cells, monocytes and NK cells. The engagement and activation of antigen receptors on B and T lymphocytes initiates signal transducing pathways. These pathways are vital for the successful initiation of an adaptive immune response and it is following the cross-linking of the B cell receptor (BCR) or high-affinity FE receptors of myeloid cells, that the tyrosine phosphorylation of NTAL occurs, enabling the formation of complexes between NTAL and further signaling molecules Grb2, Gab1, Sos1 and c-Cbl. Antigen-initiated activation events include tyrosine phosphorylation of two transmembrane adaptor proteins, LAT and NTAL; however NTAL is functionally and topographically different from LAT. Recent studies have indicated, that the evidence for functional homology between LAT and NTAL/LAB, and the possibility that the differences between them might lead to specific clinical therapeutics to manipulate immune cell responses.

General readings

1. Yamasaki S, Ishikawa E, Sakuma M, Kanagawa O, Cheng AM, Malissen B, Saito T.: LAT and NTAL mediate immunoglobulin E-induced sustained extracellular signal-regulated kinase activation critical for mast cell survival. Mol Cell Biol. 2007 Jun;27(12):4406-15.
2. Brdicka T, Imrich M, Angelisova P, Brdickova N, Horvath O, Spicka J, Hilgert I, Luskova P, Draber P, Novak P, Engels N, Wienands J, Simeoni L, Osterreicher J, Aguado E, Malissen
M, Schraven B, Horejsi V.: Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling. J Exp Med. 2002 Dec 16; 196 (12):1617-26.
4. Tkaczyk C, Horejsi V, Iwaki S, Draber P, Samelson LE, Satterthwaite AB, Nahm DH, Metcalfe DD, Gilfillan AM.: NTAL phosphorylation is a pivotal link between the signaling cascades leading to human mast cell degranulation following Kit activation and Fc epsilon RI aggregation. Blood. 2004 Jul 1;104(1):207-14.
5. Volna P, Lebduska P, Draberova L, Simova S, Heneberg P, Boubelik M, Bugajev V, Malissen B, Wilson BS, Horejsi V, Malissen M, Draber P.: Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL. J Exp Med. 2004 Oct 18;200(8):1001-13.
6. Lebduska P, Korb J, Tùmová M, Heneberg P, Dráber P.: Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non-adherent mast cells. J Immunol Methods. 2007 Dec 1;328(1-2):139-51.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

LAT2 / WBSCR15 antibody

Subcellular localization of NTAL by confocal microscopy in THP-1 human acute monocytic leukemia cell line. THP-1 cells were permeabilized and immunostained using anti-NTAL (NAP-07; green). Nuclei are vizualized by propidium iodide (red). Mouse IgG1 NAP-07 Purified Hu, Ms F, ICC/IF, IP, WB
0.1 mg / €260.00
  Acris Antibodies GmbH

LAT2 / WBSCR15 antibody

Staining of Human Peripheral Lymphocytes lysate using LAT2 Antibody Cat.-No AP32820PU-N (0.01 µg/ml) (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

LAT2 / WBSCR15 antibody

Anti-LAT2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. Mouse IgG2a P-03 Purified Hu
50 µg / €345.00
  OriGene Technologies, Inc.

LAT2 / WBSCR15 antibody

Western blot analysis of NTAL expression in rat kidney tissue lysate. Rabbit Purified Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

LAT2 / WBSCR15 antibody

Predicted cell location: Cytoplasm. Positive control: Human colon cancer tissue. Recommended dilution: 1/10-50  The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using LAT2 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

LAT2 / WBSCR15 antibody

Immunohistochemistry staining of human spleen (paraffin sections) with anti-NTAL polyclonal. Rabbit Aff - Purified Hu P, WB
0.1 mg / €260.00
  Acris Antibodies GmbH

LAT2 / WBSCR15 antibody

Figure 1. Western Blotting analysis (non-reducing conditions) of mouse NTAL in mouse spleen tissue:  Lane 1: immunostaining with polyclonal anti-NTAL antibody (AP03007PU-N); Lane 2: immunostaining with polyclonal anti-mouse NTAL antibody (specific for N-terminal domain of mouse NTAL). Rabbit Aff - Purified Ms WB
0.1 mg / €260.00
  Acris Antibodies GmbH

LAT2 / WBSCR15 antibody

Distribution of LAT and NTAL in separate clusters on mast cell plasma membrane sheets. LAT and NTAL were detected on mouse bone marrow-derived mast cells (BMMC; 2A) and RBL rat basophilic leukemia cell line (2B) by anti-LAT (LAT-1D1; AM03086PU-N) and polyclonal anti-NTAL antibody, followed by 5 or 10 nm gold-conjugated secondary antibodies, respectively. Rabbit Aff - Purified Ms, Rt ICC/IF, WB
0.1 mg / €260.00
  Acris Antibodies GmbH

LAT2 / WBSCR15 (91-243) antibody

Spleen, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

LAT2 / WBSCR15 (Center) antibody

Western blot analysis in Hela cell line lysates (35ug/lane) using LAT2 / WBSCR15 antibody Cat.-No. AP52448PU-N. This demonstrates the LAT2 antibody detected the LAT2 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

LAT2 / WBSCR15 antibody

Subcellular localization of NTAL by confocal microscopy in THP-1 human acute monocytic leukemia cell line.
THP-1 cells were permeabilized and immunostained using anti-NTAL (NAP-07; green). Nuclei are vizualized by propidium iodide (red). Mouse IgG1 NAP-07 PE Hu, Ms F
0.1 mg / €410.00
  Acris Antibodies GmbH

LAT2 / WBSCR15 antibody

LAT2 / WBSCR15 Mouse IgG1 NAP-03 Purified Hu IP, P, WB
0.1 mg / €260.00
  Acris Antibodies GmbH

LAT2 / WBSCR15 antibody

Subcellular localization of LAT2 by confocal microscopy in THP-1 human acute monocytic leukemia cell line. THP-1 cells were permeabilized and immunostained using LAT2 monoclonal antibody, clone NAP-07 ( Cat # MAB0946 ; green ) . Nuclei are vizualized by propidium iodide ( red ) . Mouse IgG1 NAP-07 Hu, Ms F, ICC/IF, IP, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

LAT2 / WBSCR15 antibody

LAT2 / WBSCR15 Mouse IgG1 NAP-07 Hu, Ms F, ICC/IF, IP, WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

LAT2 / WBSCR15 antibody

Western blotting analysis ( non-reducing conditions ) of LAT2-transfected HEK-293 FT cells using LAT2 polyclonal antibody ( Cat # PAB1261 ). Lane 1 : Cell lysate of transfected HEK-293 FT cells. Lane 2 : Cell lysate of non-transfected HEK-293 FT cells ( negative control ). ( HEK-293 FT is a subclone of the primary human embryonal kidney epithelial cell line. ) Rabbit Hu WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

LAT2 / WBSCR15 antibody

Western blotting analysis ( non - reducing conditions ) of mouse Lat2 in mouse spleen tissue. Lane 1 : Immunostaining with Lat2 polyclonal antibody ( Cat # PAB1262 ) . Lane 2 : Immunostaining with Lat2 polyclonal antibody ( specific for N - terminal domain of mouse Lat2 ) . Rabbit Ms WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

LAT2 / WBSCR15 antibody

Western blotting analysis ( non - reducing conditions ) of mouse Lat2 in mouse spleen tissue. Lane 1 : Immunostaining with Lat2 polyclonal antibody ( Cat # PAB1263 ) . Lane 2 : Immunostaining with Lat2 polyclonal antibody ( specific for N - terminal domain of mouse Lat2 ) . Rabbit Ms, Rt ICC/IF, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

LAT2 / WBSCR15 antibody

Western blot analysis of Lat2 expression in rat kidney tissue lysate. Rabbit Rt WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

LAT2 / WBSCR15 antibody

LAT2 / WBSCR15 Rabbit IgG Aff - Purified Hu WB
0.1 mg / €290.00
  Boster Immunoleader

LAT2 / WBSCR15 antibody

Immunocytochemistry: NTAL Antibody (NAP-07) [NB500-341]- Subcellular localization of NTAL by confocal microscopy in THP-1 human acute monocytic leukemia cell line.  THP-1 cells were permeabilized and immunostained using anti-NTAL (NAP-07; green). Nuclei are vizualized by propidium iodide (red). Mouse IgG1 NAP-07 Aff - Purified Hu, Ms F, ICC/IF, IP, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

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