Layilin antibody

Principal name

Layilin antibody

Alternative names for Layilin antibody

LAYN

SwissProt ID

Q6UX15 (Human)

Gene ID

143903 (LAYN)

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F)

Background of Layilin antibody

Layilin is a widely expressed integral membrane hyaluronan receptor and a transmembrane protein with homology to C-type lectins. This protein interacts with the ERM protein family of cytoskeletal membrane linker molecule members, including talin, ezrin, moesin, and radixin. The carboxy-terminal domain of layilin is capable of binding to the amino-terminal domain of radixin. An interdomain interaction between the amino- and the carboxy-terminal domains of radixin inhibits its ability to bind to layilin. Layilin plays an important role in cell adhesion and motility. It is important for the maintenance of the cortical structure in the cell because it mediates signals from the extracellular matrix to the cytoskeleton. In spreading cells, Layilin acts as a membrane-binding site for talin in the peripheral ruffles, and it may play a role in the migration of cells to a wound site.

General readings

Bono P., et al. (2005) Exp Cell Res 1;308(1): 177-87Mark L., et al. (1998) The Journal of Cell Biology 143(2): 429

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Layilin antibody

Predicted band size: 43 kDa . Positive control: Mouse brain tissue lysate. Recommended dilution: 1/200-1000 Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Layilin antibody

WB Suggested Anti-LAYN Antibody Titration: 0.2-1 ug/ml; Positive Control: MCF7 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Layilin antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LAYN (RC206197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LAYN. Mouse IgG1 OTI4C11 Purified Hu F, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Layilin antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LAYN (RC206197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LAYN. Mouse IgG1 OTI4D3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Layilin antibody

The cell lysates of A549 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LAYN antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b AT20G8 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Layilin antibody

The cell lysates of A549 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LAYN antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b AT20G8 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

Layilin antibody

LAYN MaxPab polyclonal antibody. Western Blot analysis of LAYN expression in human liver. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Layilin antibody

Human MCF-7; WB Suggested Anti-LAYN Antibody Titration: 0.2-1 ug/ml. Positive Control: MCF7 cell lysate; LAYN antibody - N-terminal region (ARP53385_P050) in Human MCF-7 cells using Western Blot Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

Layilin antibody

Western BlotLayilin  Antibody [NBP1-69660] - This Anti-LAYN antibody was used in Western Blot of MCF7 tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

Layilin antibody

Immunohistochemistry-Paraffin: LAYN Antibody [NBP1-83541] - Immunohistochemical staining of human stomach, lower shows strong cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Layilin

Layilin Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Layilin (22-235, His-tag)

Layilin Human Purified > 85 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

Layilin (22-235, His-tag)

Layilin Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Layilin

Layilin Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

Layilin

Layilin Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

LAYN 293T Cell Transient Overexpression Lysate(Denatured)

LAYN 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

LAYN overexpression lysate

LAYN overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

17 Item(s)

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