LC3 antibody

Principal name

LC3 antibody

Alternative names for LC3 antibody

MAP1A / 1B light chain 3 A, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1 light chain 3-like protein 1, MAP1LC3A, LC3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, Microtubule-associated proteins 1A/1B light chain 3A, Microtubule-associated protein 1 light chain 3 alpha

SwissProt ID

Q2HJ23 (Bovin), Q6XVN8 (Rat), Q91VR7 (Mouse), Q9H492 (Human), Q9H492-2

Gene ID

84557 (MAP1LC3A)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), African clawed frog, Op (Opossum), Prim (Primate)

Available hosts

Rabbit, Goat, Mouse

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Frozen Sections (C), Dot blot (Dot)

Background of LC3 antibody

LC3 (microtubule-associated protein light chain 3), the most studied autophagy biomarker, was origilly identified as a subunit of microtubule-associated proteins 1A and 1B (MAP1LC3) and was later found to contain similarity to yeast protein Apg8/Aut7/Cvt5. Distributed ubiquitously in eukaryotes, LC3 is expressed as 3 splice variants/isoforms (LC3A, LC3B and LC3C) which undergo post-translatiol processing, wherein, the unprocessed form of LC3 is proteolytically cleaved by Atg4 protease to form LC3-I with carboxytermil exposed glycine. During autophagy, this exposed glycine of LC3-I is conjugated by Atg7 (an E1-like activity), Atg3 (an E2-like conjugating activity) and by Atg12-Atg5-Atg16L multimers (E3-like ligase activity) to phosphatidylethanolamine (PE) moiety for generating LC3-II. The lipophilic character of PE group facilitates LC3-II insertion into autophagosomes membranes, and as a result LC3-II is degraded when autophagosomes fuse with lysosomes to form autolysosomes for lysus of intra-autophagosomal components by lysosomal hydrolases. Conversion of LC3I to LC3II when correlated with autophagosome numbers is considered as the best marker of autophagy because LC3-II is the only well-characterized protein which specifically localize to autophagic structures throughout autophagy (from phagophore to lysosomal degradation). LC3 is a great tool in research as autophagy is implicated in numerous physiological/pathological processes including responses to exercise/aging, cancer, metabolic and neurodegenerative disorders, and cardiovascular/pulmory diseases.

General readings

Settembre, C., et al. A block of autophagy in lysosomal storage disorders. Hum. Mol. Genet. 17(1): 119-129, 2008

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

LC3 pSer12 antibody

Something like SH-SY5Y cells expressing GFP-LC3-WT or-S12D treated with rapamycin or vehicle for 1h. Rabbit Ig Aff - Purified Hu Dot, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

LC3 antibody

Western blot analysis of anti-LC3 Mab (Cat. #TA324327) at 8 ug/ml. Lane 1: Y79 (soluble fraction of cell extract); Lane 2: 293 transfected with human LC3 (whole cell extract). Mouse IgG1 166AT1234 Purified Hu, Ms, Rt ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

LC3 (N-term) antibody

Western blot analysis of anti-LC3 (APG8a) Pab (Cat. #TA324624) in rat brain lysate. Both lipidated (arrow, II) and non-lipidated APG8a (arrow, I) were detected in membrane fraction (P) but only non-lipidated LC3 was detected in soluble fraction (S). Rabbit Ig Purified Hu, Ms, Rt ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

LC3 Cleaved antibody

Western blot analysis of anti-cleaved-LC3 (APG8a) Pab (Cat. #TA324626) in mouse brain tissue lysate. Cleaved-LC3 (APG8a) was detected using the purified Pab. Rabbit Ig Purified Hu, Ms ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

LC3 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MAP1LC3A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803577) Mouse IgG2a OTI10H6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

LC3 antibody

Predicted cell location: Cytoplasm . Positive control: Human colon and gastric cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using MAP1LC3A antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

LC3 antibody

Predicted cell location: Cytoplasm . Positive control: Human liver and lung cancer tissue . Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using MAP1LC3A antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

LC3 antibody

Western blot analysis of MAP1LC3A in HeLa cell lysate with MAP1LC3A antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide. Rabbit IgG Purified Hu ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

LC3 antibody

Western blot analysis of extracts of various cell lines, using MAP1LC3A antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

LC3 antibody

Western Blot: LC3 Antibody [TA336735] - Human brain lysate. Rabbit Purified Bov, Hu, Ms, Rt, Ze C, F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

LC3 antibody

Western Blot: LC3 Antibody [TA336967] - Lysate from human brain in the 1) absence, 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain probed with ATG8 antibody at 1 ug/ml. Rabbit IgG Purified Bov, Hu, Ms, Op, Prim, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

LC3 antibody

Western blot detection of LC3A in CHO-K1 cell lysate(B)and CHO-K1 transfected by LC3A (A)cell lysate using LC3A mouse mAb (1:2000 diluted). Mouse IgG1 4C8-B7-B9 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

LC3 antibody

Western blot analysis of MAP1LC3 in human brain tissue lysate with MAP1LC3 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

LC3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP1LC3A (RC202222, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP1LC3A. Mouse IgG1 OTI2D5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

LC3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP1LC3A (RC202222, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP1LC3A. Mouse IgG1 OTI4C5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

LC3 antibody

Immunofluorescence staining of methanol-fixed HeLa cells using LC3A antibody (Red). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

LC3 antibody

Immunofluorescence staining of methanol-fixed HeLa cells using LC3A antibody (Red). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
50 µl / €230.00
  Acris Antibodies GmbH

LC3 (97-106) antibody

Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat IgG Aff - Purified Av, Bear, Bov, Bt, Can, Elephant, Eq, Hst, Hu, Insect, L, Mky, Ms, Op, Por, Prim, Rt, Xen P
50 µg / €470.00
  Acris Antibodies GmbH

LC3 antibody

Western blot analysis of LC3 (APG8a) (arrow) using LC3 Antibody Cat.-No AP32160PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the LC3 (APG8a) gene (Lane 2) (Origene Technologies). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

LC3 (D106) antibody

SY5Y cells were pretreated with 5nM bafilomycin for 24hr and fixed in methanol. Treatment with Autophagy LC3 Antibody (APG8a) (D106) Cat.-No AP32164PU-N at dilution 1/100. Data courtesy of Jianhui Zhu, MD, PhD & Charleen T. Chu, MD, PhD, University of Pittsburgh School of Medicine. Rabbit Ig Aff - Purified Hu ICC/IF, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

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