LC3B antibody

Principal name

LC3B antibody

Alternative names for LC3B antibody

MAP1LC3B, MAP1A/MAP1B, Map1lc3b, Map1alc3, Map1lc3, Microtubule-associated protein 1 light chain 3 beta, Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1A / 1B light chain 3 B

SwissProt ID

O41515 (Bovin), Q62625 (Rat), Q9CQV6 (Mouse), Q9GZQ8 (Human)

Gene ID

81631 (MAP1LC3B), 67443 (Map1lc3b), 64862

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Xen (Xenopus), Ze (Zebrafish), Can (Canine), Hst (Hamster), Mky (Monkey), Por (Porcine), Prim (Primate)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Flow Cytometry (F), Dot blot (Dot)

Background of LC3B antibody

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3b is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.

General readings

Hiroshi Aoki, Yasunari Takada, Seiji Kondo, Raymond Sawaya, Bharat Aggarwal, and Yasuko Kondo, Evidence That Curcumin Suppresses the Growth of Malignant Gliomas In Vitro and In Vivo Through Induction of Autophagy: Role of Akt and ERK Signaling Pathway, Mol Pharmacol. 2007 Mar 29; [Epub ahead of print]

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

LC3B (N-term) (incl. pos. control) antibody

Endogenous LC-3 punctae detected with AM20212PU-N LC3 antibody (Clone 5F10): The majority of LC-3 was diffusely localized in unstimulated COS-7 cells, wheres punctated signals of LC-3 increase after induction of autophagy by vinblastin stimulation for 2 hr. Cells were fixed with paraformaldehyd followed by methanol treatment. Cells were permeabilized with 0.3% TritonX100. Endogenous LC-3 was detected with mab 5F10. 
Images by courtesy of I. Ciechomska and A. Tolkovsky, University of Cambridge, UK. Mouse IgG1 5F10 Purified Can, Hst, Hu, Ms, Rt ICC/IF, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

LC3B antibody

Detection of LC3 in mouse ES cell lysates Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

LC3B antibody

Detection of LC3B in treated U87-MG (human glioblastoma astrocytoma) lysates Rabbit IgG Purified Hu, Ms F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

LC3B (N-term) antibody

Immunofluorescent analysis of U251 cells, using LC3 Antibody (APG8B)(N-term)(Cat. #TA324625). U251 cells(right) were treated with Chloroquine (50 μM,16h). TA324625 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue). Rabbit Ig Purified Hu, Rt ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

LC3B antibody

Western Blot: LC3I Antibody [TA336849] - WB analysis of LC3I in Neuro2A cell lysate. Rabbit Purified Bov, Hu, Ms, Por, Prim, Rt C, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

LC3B antibody

Western blot analysis using MAP1LC3B mAb against human MAP1LC3B (AA: 1-125) recombinant protein. (Expected MW is 40.2 kDa) Mouse IgG1 5H12 Ascites Hu E, F, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

LC3B antibody

Detection of autophagic LC3 in mouse ES cell lysate.. The atg5-/- lane (ES cells, cultured to form embryonic bodies, that are deficient in conversion of LC3-1 to LC3-11) demonstrates the specificity of NB 100-2331, as there is no detection of LC3-11. Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

LC3B antibody

Predicted band size: 14 kDa . Positive control: K-562, Hela, Jurkat and 293 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 12%SDS-PAGE Lane1,2,3,4: K-562, Hela, Jurkat and 293 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

LC3B antibody

Western blot analysis of MAP1LC3B in human brain tissue lysate with MAP1LC3B antibody at 1 ug/ml. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

LC3B antibody

Immunohistochemical analysis of paraffin-embedded breast carcinoma tissue using Phospho-Akt(Ser473) Antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

LC3B (N-term) (incl. pos. control) antibody

LC3B Mouse IgG1 5F10 Biotin Can, Hst, Hu, Ms, Rt ICC/IF, WB
0.1 mg / €490.00
  Acris Antibodies GmbH

LC3B (N-term) (incl. pos. control) antibody

Detection of endogenous LC-3: Whole cell lysates of untreated tumor cells were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with AM20213PU-N LC3 antibody (Clone: 2G6) at 0.5 µg/ ml for 1h at RT and developed by ECL (exp. time: 30 sec).
Lane 1: HeLa 
Lane 2: HepG2 
Lane 3: HEK 293 
Lane 4: SH-SY5Y
Lane 5: MDCK
Lane 6: PC12 
Lane 7: CMT
Lane 8: Neuro2A
Lane 9: NIH-3T3 Mouse IgG1 2G6 Purified Hst, Hu, Mky, Ms, Rt ICC/IF, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

LC3B (incl. pos. control) antibody

LC3B Mouse IgG1 5H3 Purified Hu ICC/IF, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

LC3B Cleaved antibody

Time course study of mouse leukaemic monocyte macrophage cells treated with U18666A, a drug that causes cholesterol and lipid storage in cells, thereby blocking fusion between late endosomes and lysosomes. Cleaved-LC3B Antibody (APG8b) Cat.-No AP32168PU-N detected punctuate staining indicative of autophagic vacuole or phagosome structures. Data courtesy of Dr. Barry Boland, Department of Pharmacology, Oxford University. Rabbit Ig Purified Hu, Ms ICC/IF
0.4 ml / €370.00
  Acris Antibodies GmbH

LC3B (C-term) antibody

Formalin-Fixed, Paraffin-embedded human testis tissue reacted with Autophagy LC3B Antibody (C-term) Cat. AP32195PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

LC3B pThr12 antibody

Dot blot analysis of Phospho-LC3 (APG8b)- Thr12 Antibody (Cat.-No AP32197PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 µg/ml. Rabbit Ig Purified Hu Dot
0.4 ml / €370.00
  Acris Antibodies GmbH

LC3B (1-30) antibody

Western blot analysis of MAP1LC3B (arrow) using LC3B Antibody (T6) Cat.-No AP32203PU-N in Y79 cell line lysates and mouse brain tissue lysates (35 µg/lane). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP1LC3B gene (Lane 2) (Origene Technologies). Rabbit Ig Aff - Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

LC3B antibody

LC3B Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

LC3B antibody

LC3B Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

LC3B antibody

Detection limit for recombinant GST tagged MAP1LC3B is approximately 0.03ng/ml as a capture antibody. Mouse IgG2b 4E11 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

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