LCK antibody

Principal name

LCK antibody

Alternative names for LCK antibody

p56-LCK, LSK, Proto-oncogene tyrosine-protein kinase LCK, Lymphocyte cell-specific protein-tyrosine kinase, T cell-specific protein-tyrosine kinase

SwissProt ID

P06239 (Human), P06240 (Mouse), P42683 (Chick), Q01621 (Rat)

Gene ID

3932 (LCK), 16818 (Lck)

Available reactivities

Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Sh (Sheep)

Available hosts

Rabbit, Mouse, Goat

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Frozen Sections (C), EPair for Elisa (EPair)

Background of LCK antibody

Lck is a lymphoid specific cytosolic protein tyrosine kinase (PTK), which is essential for T cell development and function. It is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T cell antigen receptor (TCR) linked signal transduction pathways. Association of the TCR with a peptide antigen bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated Lck to the vicinity of the TCR/CD3 complex. Lck then phosphorylates tyrosine residues within the immunoreceptor tyrosine based activation motifs (ITAMs) in the cytoplasmic tails of the TCR chains and CD3 subunits. The phosphoITAMs serve as docking sites for Src homology domain 2 (SH2) containing molecules, predominantly ZAP 70 and Syk. Only then can ZAP 70 undergo tyrosine phosphorylation, become enzymatically active and further phosphorylate downstream effector molecules. In addition, Lck contributes to signaling by other receptor molecules. Lck is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature TCR. Lck (p56lck), a member of the Src family of non-receptor tyrosine protein kinases, is expressed predominantly in T cells. Lck function is critical both for T cell development in the thymus and activation of mature T cells in the periphery by antigen. The activity of Lck is known to be regulated by phosphorylation of two conserved tyrosine residues, Tyr-505 (equivalent to Tyr-529 in c-Src) and Tyr-394 (equivalent to Tyr-418 in c-Src).

General readings

1. Mingjian Shi, John C. Cooper, Chao-Lan Yu
Mol. Cancer Res., Jan 2006; 4: 39 ¨C 45
2. Robert F. Stachlewitz, Michelle A. Hart, Brian Bettencourt
J. Pharmacol. Exp. Ther., Oct 2005; 315: 36 ¨C 41
3. Ibrahim Y. Hawash, Kamala P. Kesavan, Anthony I. Magee
J. Biol. Chem., Feb 2002; 277: 5683 ¨C 5691

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

LCK antibody

Western Blotting analysis (reducing conditions) of Human Lck in whole cell lysate using anti-Human Lck (LCK-01). 
Lane 1: J. CaM-1.6 cell line (a mutant derivate of the JURKAT cell line) transfected with Lck. 
Lane 2: HEK-293T cell line transfected with Lck. 
Lane 3: HEK-293T cell line (non-transfected). Mouse IgG1 LCK-01 Purified Hu F, ICC/IF, IP, WB
0.1 mg / €240.00
  Acris Antibodies GmbH

LCK antibody

Western blot analysis of LCK over-expressed 293 cell line, cotransfected with LCK Validated Chimera RNAi ( Cat # H00003932-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with LCK monoclonal antibody (M01) clone 3F7-F5 (Cat # H00003932-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. Mouse IgG1 3F7-F5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

LCK (39-52) antibody

(0.1ug/ml) staining of Human Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

LCK antibody

Western blot analysis of extracts from Jurkat cells, using LCK (Ab-59) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

LCK antibody

Predicted band size: 58 kDa . Positive control: Jurkat cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lysate: 30 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

LCK antibody

LCK Antibody (C-term) (Cat. #TA324741) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the LCK antibody detected the LCK protein (arrow). Rabbit IgG Purified Hu F, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

LCK antibody

LCK Antibody(Cat. #TA324963) western blot analysis in HL-60,Jurkat,NCI-H292 cell line lysates (35ug/lane).This demonstrates the LCK antibody detected the LCK protein (arrow). Mouse IgG1 845CT3.5.2 Ascites Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

LCK (N-term) antibody

Western blot analysis of lysates from mouse thymus and rat thymus tissue lysate(from left to right), using LSK Antibody (I37)(Cat.  #TA324976).  TA324976 was diluted at 1:1000 at each lane.  A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

LCK antibody

Western blot analysis of Lck expression in Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

LCK antibody

Western blot analysis of Lck expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

LCK antibody

Western blot detection of LCK in Jurkat and Ramos cell lysates and using LCK mouse mAb (1:1000 diluted).Predicted band size: 58KDa.Observed band size: 58KDa. Mouse IgG1 3E5-C8-B9 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

LCK antibody

Figure 1.Western blot of jurkat cell lysate using anti-p56 lck antibody (AM05306PU-N). Mouse IgG2b 3A5 Purified Hu, Ms, Rt E, IP, WB
0.1 mg / €570.00
  Acris Antibodies GmbH

LCK antibody

Western blot analysis using LCK antibody Cat.-No AM06133SU-N against MOLT-4 (Lane 1), CCRF-CEM (Lane 2), CCRF-HSB-2 (Lane 3) and Jurkat (Lane 4) cell lysate. Mouse IgG2a 8E5F9 Ascites Hu E, WB
0.1 ml / €440.00
  Acris Antibodies GmbH

LCK antibody

Thymus: Formalin-Fixed Paraffin-Embedded (FFPE) using ant-Lck antibody. Mouse IgG1 33D196 Purified Hu P, WB
50 µl / €470.00
  Acris Antibodies GmbH

LCK control peptide

LCK Purified
50 µg / €190.00
  Acris Antibodies GmbH

LCK antibody

Western blot analysis of Lck expression in rat kidney tissue lysate. Rabbit Aff - Purified Chk, Hu, Ms, Rt IP, P, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

LCK pTyr505 antibody

LCK Rabbit Aff - Purified Hu IP, P, WB
0.1 mg / €480.00
  Acris Antibodies GmbH

LCK pTyr394 antibody

Western blot analysis of extracts from JK cells untreated or treated with starvation using Lck (Phospho-Tyr394) Antibody AP02429PU-N Rabbit Aff - Purified Hu, Ms WB
0.1 ml / €320.00
  Acris Antibodies GmbH

LCK pTyr394 antibody

Western blot analysis of extracts from JK cells untreated or treated with starvation using Lck (Phospho-Tyr394) Antibody AP02429PU-N Rabbit Aff - Purified Hu, Ms WB
50 µl / €240.00
  Acris Antibodies GmbH

LCK antibody

Western blot (WB) analysis of Lck antibody (Cat.-No.: AP06534PU-N) at 1/500 dilution Lane1:Hela whole cell lysate Lane2:Mouse kidney tissue lysate Lane3:Rat liver tissue lysate Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

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