LCP2 antibody

Principal name

LCP2 antibody

Alternative names for LCP2 antibody

LCP2, Lymphocyte cytosolic protein 2, SLP-76 tyrosine phosphoprotein, SLP76, SH2 domain-containing leukocyte protein of 76 kDa

SwissProt ID

P07187 (Drome), P91629 (Dromi), Q13094 (Human), Q60787 (Mouse)

Gene ID

3937 (LCP2)

Available reactivities

Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Bov (Bovine), Can (Canine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Enzyme Immunoassay (E), Flow Cytometry (F)

Background of LCP2 antibody

SLP-76 was origilly identified as a substrate of the ZAP-70 protein tyrosine kise following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. The SLP-76 locus has been localized to human chromosome 5q33 and the gene structure has been partially characterized in mice. The human and murine cDs both encode 533 amino acid proteins that are 72% identical and comprised of three modular domains. The NH2-terminus contains an acidic region that includes a PEST domain and several tyrosine residues which are phosphorylated following TCR ligation. SLP-76 also contains a central proline-rich domain and a COOH-termil SH2 domain. A number of additiol proteins have been identified that associate with SLP-76 both constitutively and inducibly following receptor ligation, supporting the notion that SLP-76 functions as an adaptor or scaffold protein. Studies using SLP-76 deficient T cell lines or mice have provided strong evidence that SLP-76 plays a positive role in promoting T cell development and activation as well as mast cell and platelet function.

General readings

1. Pivniouk VI, Martin TR, Lu-Kuo JM, Katz HR, Oettgen HC, Geha RS: SLP-76 deficiency impairs signaling via the high-affinity IgE receptor in mast cells. J Clin Invest. 1999 Jun;103(12):1737-43.
2. Silverman MA, Shoag J, Wu J, Koretzky GA: Disruption of SLP-76 interaction with Gads inhibits dynamic clustering of SLP-76 and FcepsilonRI signaling in mast cells. Mol Cell Biol. 2006 Mar;26(5):1826-38.
3. Koretzky GA, Abtahian F, Silverman MA: SLP76 and SLP65: complex regulation of signalling in lymphocytes and beyond. Nat Rev Immunol. 2006 Jan;6(1):67-78.
4. Beach D, Gonen R, Bogin Y, Reischl IG, Yablonski D: Dual role of SLP-76 in mediating T cell receptor-induced activation of phospholipase C-gamma1. J Biol Chem. 2007 Feb
2;282(5):2937-46.
6. Bogin Y, Ainey C, Beach D, Yablonski D: SLP-76 mediates and maintains activation of the Tec family kinase ITK via the T cell antigen receptor-induced association between SLP-76 and ITK. Proc Natl Acad Sci U S A. 2007 Apr 17;104 (16):6638-43.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

LCP2 (N-term) antibody

  Goat Polyclonal Antibody against LCP2  
TA302947 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

LCP2 antibody

  Rabbit anti-LCP2 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using LCP2 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

LCP2 antibody

Western Blotting analysis (reducing conditions) of whole cell lysate using anti-SLP76 (SLP-76/03). Lane 1: HPB-ALL human peripheral blood T cell leukemia cell line. Lane 2: JURKAT human peripheral blood T cell leukemia cell line. Lane 3: RAMOS human Burkitt lymphoma cell line. Mouse IgG2a AS55 Purified Hu IP, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

LCP2 antibody

LCP2 Mouse IgG2a H76 FITC Hu, Ms F
100 Tests / €440.00
  Acris Antibodies GmbH

LCP2 antibody

MOLT4 total lysate (1% NP40) was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-SLP76 antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Mouse IgG2a H76 Aff - Purified Hu, Ms F, IP, WB
0.2 ml / €390.00
  Acris Antibodies GmbH

LCP2 antibody

Immunohistochemistry (IHC) analyzes of SLP-76 antibody (Cat.-No.: AP21127PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

LCP2 (216-434) antibody

Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Serum Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

LCP2 antibody

Western Blotting analysis (reducing conditions) of whole cell lysate using anti-SLP76 (SLP-76/03). Lane 1: HPB-ALL human peripheral blood T cell leukemia cell line. Lane 2: JURKAT human peripheral blood T cell leukemia cell line. Lane 3: RAMOS human Burkitt lymphoma cell line. Mouse IgG2b SLP-76/03 Purified Hu, Ms, Por P, WB
0.1 mg / €260.00
  Acris Antibodies GmbH

LCP2 antibody

Immunohistochemistry staining of human thymus (paraffin sections) with anti-SLP76 polyclonal. Rabbit Purified Hu P, WB
0.1 mg / €260.00
  Acris Antibodies GmbH

LCP2 pTyr128 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using SLP-76 (Phospho-Tyr128) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €230.00
  Assay Biotech

LCP2 pTyr128 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using SLP-76 (Phospho-Tyr128) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €330.00
  Assay Biotech

LCP2 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using SLP-76 (Ab-128) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €230.00
  Assay Biotech

LCP2 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using SLP-76 (Ab-128) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

LCP2 - human gene knockout kit via CRISPR

LCP2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

LCP2 - human gene knockout kit via CRISPR

LCP2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

LCP2 - human gene knockout kit via CRISPR

LCP2 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

LCP2 - human gene knockout kit via CRISPR

LCP2 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

LCP2

  Recombinant protein of human lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa) (LCP2)  
LCP2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

LCP2 overexpression lysate

LCP2 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, LCP2 (mGFP-tagged) - Human lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa) (LCP2), 200 uL, >10^7 TU/mL

Lenti ORF particles, LCP2 (mGFP-tagged) - Human lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa) (LCP2), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

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