LCT antibody

Principal name

LCT antibody

Alternative names for LCT antibody

LCT, LPH, Lactase-phlorizin hydrolase, Lactase-glycosylceramidase

SwissProt ID

P09848 (Human), P09849 (Rabit), Q02401 (Rat)

Gene ID

3938 (LCT)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of LCT antibody

The terminal differentiation of intestinal development occurs during cytodifferentiation and the weaning transition age. Lactase-phlorizin hydrolase (Lact), liver fatty acid binding protein (Fabp1), and sucrase-isomaltase (SI) are well-characterized markers of these transitions. Lact gene expression is spatiotemporally regulated during mammalian gut development. The Zn finger transcription factor family member Gata4 and hepatocyte nuclearfac-1alfa each are indispensable for Lact and Fabp1 expression but both are dispensable for SI gene expression (1). Intestinal and renal epithelial cells are characterized by distinct apical and basolateral membrane domains that are separated by tight junctions and the establishment and maintenance of this polarity depend on specific gene expression and protein targeting to their correct location. One of the protein responsible for correct targeting of proteins is galactic-3, a member of conserved family of lectin proteins. The transport defects in the intestinal brush border Lact in galactin-3 null mutant mice suggest that Lact trafficking involves direct interaction with galactin-3 it transit thru noon-raft-dependent apical transport platform. Deficiency of intestinal lactase, the enzyme required for lactose digestion, can result in symptoms of gastrointestinal malabsorption, or lactose intolerance. Congenital lactase deficiency (CLD) is a cause of disaccharide intolerance and malabsorption characterized by watery diarrhea in infants fed breast milk or lactose-containing formulas. Although the exact cause of CLD is not known, mutations in the coding region of brush border Lact are found to cause CLD in a Finnish study. A mutant Lact (Glycine 1363 was replaced by serine) when expressed in Cos-1 cells resulted in misfolded, enzymatically inactive protein that accumulated in ER. The activity and trafficking is partially restored by expression at permissive temperature (2). Several mutations have been observed in Lactase gene among European populations. A single nucleotide substitution leading to an amino acid change S688 to P in exon 7 and E 1612 to X in exon 12 are found in Italian hypolactasia patients. Five base deletion V565 fsX567 leading to stop codon inexon 6 was found in one and E1612 to X in Exxon 12 was noted in Finnish patents.
In pre weaning rats the intestinal lactase is found in both membrane bound and soluble forms, the distribution and post-translational modifications depend upon hormones, thyroxine and cortisone levels (4). The post natal development of intestine is associated with decrease in particulate Lact activity partly due to proteolysis by luminal proteases, a condition commonly associated with hypolactasia in human (4, 5). Lact is expressed in at least 2 splice forms with apparent MW ranging from 200-240kDa. The Lact has at least 3 “Glycosyl hydrolase family 1” motifs spaced though out the protein. Lact has a single membrane anchor domain near the C-terminal end and has 7 putative asparagines linked glycosylation sites.

7 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

LCT Control Peptide

LCT
0.25 mg / €250.00
  Acris Antibodies GmbH

LCT (1081-1095) antibody

Western blot with AP10118PU-N. Rabbit Aff - Purified Ms, Rt WB
0.1 mg / €420.00
  Acris Antibodies GmbH

LCT antibody

LCT Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

LCT antibody

LCT Mouse IgG1 3H6 Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

LCT antibody

Immunohistochemistry-Paraffin: Lactase Antibody [NBP1-87586] Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

LCT

LCT Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

LCT

LCT Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

7 Item(s)

per page
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