LCT antibody

Principal name

LCT antibody

Alternative names for LCT antibody

LCT, LPH, Lactase-phlorizin hydrolase, Lactase-glycosylceramidase

SwissProt ID

P09848 (Human), P09849 (Rabit), Q02401 (Rat)

Gene ID

3938 (LCT)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of LCT antibody

The terminal differentiation of intestinal development occurs during cytodifferentiation and the weaning transition age. Lactase-phlorizin hydrolase (Lact), liver fatty acid binding protein (Fabp1), and sucrase-isomaltase (SI) are well-characterized markers of these transitions. Lact gene expression is spatiotemporally regulated during mammalian gut development. The Zn finger transcription factor family member Gata4 and hepatocyte nuclearfac-1alfa each are indispensable for Lact and Fabp1 expression but both are dispensable for SI gene expression (1). Intestinal and renal epithelial cells are characterized by distinct apical and basolateral membrane domains that are separated by tight junctions and the establishment and maintenance of this polarity depend on specific gene expression and protein targeting to their correct location. One of the protein responsible for correct targeting of proteins is galactic-3, a member of conserved family of lectin proteins. The transport defects in the intestinal brush border Lact in galactin-3 null mutant mice suggest that Lact trafficking involves direct interaction with galactin-3 it transit thru noon-raft-dependent apical transport platform. Deficiency of intestinal lactase, the enzyme required for lactose digestion, can result in symptoms of gastrointestinal malabsorption, or lactose intolerance. Congenital lactase deficiency (CLD) is a cause of disaccharide intolerance and malabsorption characterized by watery diarrhea in infants fed breast milk or lactose-containing formulas. Although the exact cause of CLD is not known, mutations in the coding region of brush border Lact are found to cause CLD in a Finnish study. A mutant Lact (Glycine 1363 was replaced by serine) when expressed in Cos-1 cells resulted in misfolded, enzymatically inactive protein that accumulated in ER. The activity and trafficking is partially restored by expression at permissive temperature (2). Several mutations have been observed in Lactase gene among European populations. A single nucleotide substitution leading to an amino acid change S688 to P in exon 7 and E 1612 to X in exon 12 are found in Italian hypolactasia patients. Five base deletion V565 fsX567 leading to stop codon inexon 6 was found in one and E1612 to X in Exxon 12 was noted in Finnish patents.
In pre weaning rats the intestinal lactase is found in both membrane bound and soluble forms, the distribution and post-translational modifications depend upon hormones, thyroxine and cortisone levels (4). The post natal development of intestine is associated with decrease in particulate Lact activity partly due to proteolysis by luminal proteases, a condition commonly associated with hypolactasia in human (4, 5). Lact is expressed in at least 2 splice forms with apparent MW ranging from 200-240kDa. The Lact has at least 3 “Glycosyl hydrolase family 1” motifs spaced though out the protein. Lact has a single membrane anchor domain near the C-terminal end and has 7 putative asparagines linked glycosylation sites.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

LCT antibody

  Mouse monoclonal Anti-Mlac6 Clone 4E10 10.2  
LCT Mouse IgG1 4E10 10.2 Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

LCT antibody

  Mouse monoclonal Anti-Mlac10 Clone 6G11  
LCT Mouse IgG1 6G11 Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

LCT antibody

  Mouse monoclonal Anti-Mlac9 Clone 3B6.1/2.F11  
LCT Mouse IgG2b 3B6.1/2.F11 Purified Hu IP
0.1 mg / €325.00
  OriGene Technologies, Inc.

LCT antibody

  Mouse monoclonal Anti-Mlac5 Clone 3G5.1/2.G6  
LCT Mouse IgG1 3G5.1/2.G6 Purified Rb IP
0.1 mg / €325.00
  OriGene Technologies, Inc.

LCT antibody

  Mouse monoclonal Anti-Mlac4 Clone 7C10-7.1  
LCT Mouse IgG1 7C10-7.1 Purified Hu IP
0.1 mg / €325.00
  OriGene Technologies, Inc.

LCT antibody

  Mouse monoclonal Anti-Mlac3 Clone 4C9 1.2  
LCT Mouse IgG2a 4C9 1.2 Purified Hu IP
0.1 mg / €325.00
  OriGene Technologies, Inc.

LCT antibody

  Mouse monoclonal Anti-Mbbm1 Clone VA4-20B  
LCT Mouse IgG1 VA4-20B Purified Hu ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

LCT antibody

  Mouse monoclonal Anti-Mlac8 Clone 3F4 G5E3  
LCT Mouse IgG1 3F4 G5E3 Purified Rb IP
0.1 mg / €325.00
  OriGene Technologies, Inc.

LCT Control Peptide

LCT
0.25 mg / €250.00
  Acris Antibodies GmbH

LCT (1081-1095) antibody

Western blot with AP10118PU-N. Rabbit Aff - Purified Ms, Rt WB
0.1 mg / €420.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

LCT - human gene knockout kit via CRISPR

LCT - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

LCT - human gene knockout kit via CRISPR

LCT - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

LCT - human gene knockout kit via CRISPR

LCT - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

LCT - human gene knockout kit via CRISPR

LCT - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, LCT (Myc-DDK tagged) - Human lactase (LCT), 200 uL, >10^7 TU/mL

Lenti ORF particles, LCT (Myc-DDK tagged) - Human lactase (LCT), 200 uL, >10^7 TU/mL  
200 µl / €2,280.00
  OriGene Technologies, Inc.

15 Item(s)

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