LDB1 antibody

Principal name

LDB1 antibody

Alternative names for LDB1 antibody

LDB1, CLIM2, LIM domain-binding protein 1, Nuclear LIM interactor, Carboxyl-terminal LIM domain-binding protein 2, CLIM-2, LIM domain-binding factor CLIM2, hLdb1

SwissProt ID

O42252 (Chick), P70662 (Mouse), Q86U70 (Human)

Gene ID

8861 (LDB1), 16825 (Ldb1)

Available reactivities

Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Ze (Zebrafish), African clawed frog, Xen (Xenopus), Chimp (Chimpanzee), Frog, Mky (Monkey), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ChIP assay (ChIP), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P)

Background of LDB1 antibody

LDB1 is also known as CLIM 2, LIM Domain Binding 1, NLI and Nuclear LIM Domain Interactor. The LIM-domain binding protein binds to the LIM domain of LIM homeodomain proteins which are transcriptional regulators of development. Nuclear LIM interactor (NLI) / LIM domain-binding protein 1 (LDB1) is located in the nuclei of neuronal cells during development, it is co-expressed with Isl1 in early motor neuron differentiation and has a suggested role in the Isl1 dependent development of motor neurons. It is suggested that these proteins act synergistically to enhance transcriptional efficiency by acting as co-factors for LIM homeodomain and Otx class transcription factors, both of which have essential roles in development.

General readings

Howard, P.W., et al. Biochem. Biophys. Res. Commun. 396(3):674-678(2010)
Howard, P.W., et al. Biochem. J. 429(1):127-136(2010)
Bhati, M., et al. EMBO J. 27(14):2018-2029(2008)
Ryan, D.P., et al. Proteins 70(4):1461-1474(2008)
Tran, Y.H., et al. J. Biochem. 140(1):105-119(2006)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

LDB1 antibody

LDB1 antibody [N2C3] detects LDB1 protein by western blot analysis. A. 30 ug 293T whole cell extract. B. 30 ug A431 whole cell extract. C. 30 ug HeLa whole cell extract. D. 30 ug HepG2 whole cell extract. 10 % SDS-PAGE. LDB1 antibody [N2C3] (TA308742) dil Rabbit IgG Aff - Purified Hu, Ms ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

LDB1 antibody

WB using Anti-LDB1 antibody shows detection of LDB1 protein (arrowhead) in Jurkat whole cell lysate.   Approximately 30 µg of lysate was loaded prior to separation and transfer to nitrocellulose. Primary antibody was used at a 1:1,800 dilution. The membrane was washed and reacted with a 1:20,000 dilution of DyLight™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at RT. Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). Rabbit Aff - Purified Chk, Hu, Ms, Xen E
0.1 mg / €325.00
  OriGene Technologies, Inc.

LDB1 antibody

WB Suggested Anti-LDB1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

LDB1 antibody

WB Suggested Anti-LDB1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Jurkat cell lysateLDB1 is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

LDB1 (C-term) antibody

Western blot analysis of LDB1 Antibody (C-term) Cat.-No AP52458PU-N in ZR-75-1 cell line lysates (35ug/lane). This demonstrates the LDB1 antibody detected the LDB1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

LDB1 antibody

LDB1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

LDB1 antibody

LDB1 monoclonal antibody (M02), clone 2G9 Western Blot analysis of LDB1 expression in IMR-32 ( Cat # L008V1 ). Mouse IgG2b 2G9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

LDB1 antibody

LDB1 monoclonal antibody (M03), clone 4G8 Western Blot analysis of LDB1 expression in IMR-32 ( Cat # L008V1 ). Mouse IgG2b 4G8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

LDB1 antibody

Western blot using Ldb1 polyclonal antibody ( Cat # PAB10257 ) shows detection of Ldb1 protein ( arrowhead ) in Jurkat whole cell lysate.
Approximately 30 µg of lysate was loaded prior to separation and transfer to nitrocellulose.
Primary antibody was used at a 1 : 1,800 dilution in 5% BLOTTO in PBS reacted overnight at 4°C.
The membrane was washed and reacted with a 1:20,000 dilution of DyLightTM800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature ( 800 nm channel, green ).
Molecular weight estimation was made by comparison to prestained MW markers in lane M ( 700 nm channel, red ).
Fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc. Rabbit Chimp, Chk, Frog, Hu, Ms, Rt E, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

LDB1 antibody

Human HepG2; WB Suggested Anti-LDB1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: HepG2 cell lysate; LDB1 antibody - C-terminal region (ARP37730_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified African clawed frog, Can, Chk, Hu, Ms, Por, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

LDB1 antibody

Human Jurkat; WB Suggested Anti-LDB1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Jurkat cell lysate. LDB1 is supported by BioGPS gene expression data to be expressed in Jurkat.; LDB1 antibody - middle region (ARP37731_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified African clawed frog, Can, Chk, Hu, Ms, Por, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

LDB1 antibody

Immunohistochemistry: LDB1 Antibody [NBP1-31362] - Immunohistochemical analysis of paraffin-embedded U87 Xenograft, using NBP1-31362 at 1:100 dilution. Rabbit IgG Hu, Ms ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

LDB1 antibody

Western Blot: LDB1 Antibody [NBP1-77832] detection of LDB1 protein (arrowhead) in Jurkat whole cell lysate. Approximately 30 g of lysate was loaded prior to separation and transfer to nitrocellulose. Primary antibody was used at a 1:1,800 dilution in 5% BLOTTO in PBS reacted overnight at 4C. The membrane was washed and reacted with a 1:20,000 dilution of Dye Light 800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). Rabbit Aff - Purified Hu, Ms, Rt ChIP, E, WB
0.1 mg / €510.00
  Novus Biologicals Inc.

LDB1 antibody

Western Blot: PPAR gamma Antibody [NBP1-77833] detection of PPARg2 protein in a mouse 3T3 whole cell lysate (lane 1 arrowhead).  Approximately 20 ug of lysate was loaded onto a 4-20% gradient gel followed by transfer to nitrocellulose.  Primary antibody was used at a 1:2,000 dilution in 5% BLOTTO PBS solution. The membrane was washed and reacted with a 1:10,000 dilution of Dye 800 Conjugated Affinity Purified Goat-anti-Rabbit IgG [H&L] MX10.  Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (lane M). Rabbit Aff - Purified Hu, Mky E, WB
0.1 mg / €510.00
  Novus Biologicals Inc.

LDB1 antibody

Immunohistochemistry-Paraffin: LDB1 Antibody [NBP1-85573] - Immunohistochemical staining of human stomach shows distinct nuclear and cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

LDB1

LDB1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

LDB1

LDB1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

LDB1 overexpression lysate

LDB1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

18 Item(s)

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