LDHB antibody

Principal name

LDHB antibody

Alternative names for LDHB antibody

LDHB, L-lactate dehydrogenase B chain, LDH-B, EC=1.1.1.27, LDH heart subunit, LDH-H, Renal carcinoma antigen NY-REN-46

SwissProt ID

P00336 (Pig), P00337 (Chick), P07195 (Human), P13490 (Rabit), P16125 (Mouse), P42119 (Xenla), P42123 (Rat), Q5E9B1 (Bovin)

Gene ID

3945 (LDHB), 3939 (LDHA), 281275, 407246 (LOC407246)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Chk (Chicken), Rb (Rabbit), Bov (Bovine), Por (Porcine)

Available hosts

Mouse, Rabbit, Sheep, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Immunoprecipitation (IP), Dot blot (Dot), Immunodiffusion (ID), Radioimmunoassay (R)

Background of LDHB antibody

Lactate dehydrogenase (LDH) is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). There are 5 different isoenzymes of LDH, LDH1 to LDH5, each composed of 4 subunits which may be of 2 different types - M and H subunits. These subunits are encoded by two different genes: The M subunit is encoded by gene LDHA whilst the H subunit is encoded by LDHB. Usually LDH2 is the predominant form in the serum. An LDH1 level higher than the LDH2 level suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH1, into the bloodstream). Suitable for immunoblotting (western or dot blot), ELISA, conjugation and most immunological methods requiring high titer and specificity.

General readings

1. Kopperschlager G et al. J Chromatogr B Biomed Appl.
684(1-2):25-49, 1996.
2. Rodriguez S et al. Oncogene 22(12): 1880-1891, 2003

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

LDHB antibody

Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 2H6 Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

LDHB antibody

Immunoperoxidase of monoclonal antibody to LDHB on formalin-fixed paraffin-embedded human tonsil tissue. [antibody concentration 5ug/ml] Mouse IgG1 2H6 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

LDHB antibody

Human Lung; Researcher: Dr. Crissy Dudgeon, Ph.D., CINJ. Application: IHC. Species+tissue/cell type: Human lung adenocarcinoma cell line A549. Primary antibody dilution: 1:100. Secondary antibody: Goat anti-rabbit AlexaFluor 488. Secondary antibody dilution: 1:400; LDHB Antibody - C-terminal region (ARP48210_P050) in Human Lung cells using Immunohistochemistry Rabbit Aff - Purified Can, Chk, Hu, Ms, Rb, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

LDHB antibody

Predicted band size: 37 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/ 1000-5000. (Gel: 10%SDS-PAGE Lysate: 30 ug Primary antibody: 1/1000 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

LDHB (15-25) antibody

Anti-LDHB antibody (0.3µg/ml) staining of Human Heart (A) and Human Kidney (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

LDHB (15-25) antibody

TA329084 (0.1 ug/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

LDHB antibody

The Recombinant Human LDHB (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LDHB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : Recombinant LDHB protein
Lane 2. : A549 cell lysate
Lane 3. : 293T cell lysate
Lane 4. : K562 cell lysate
Lane 5. : Jurkat cell lysate Mouse IgG2a AT14D7 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

LDHB antibody

The Recombinant Human LDHB (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LDHB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : Recombinant LDHB protein
Lane 2. : A549 cell lysate
Lane 3. : 293T cell lysate
Lane 4. : K562 cell lysate
Lane 5. : Jurkat cell lysate Mouse IgG2a AT14D7 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

LDHB antibody

LDHB Rabbit IgG Azide Free Bov Dot, E, ICC/IF, ID, IP, R, WB
10 mg / €230.00
  Acris Antibodies GmbH

LDHB antibody

LDHB Rabbit IgG Biotin Bov Dot, E, ICC/IF, ID, IP, R, WB
1 ml / €340.00
  Acris Antibodies GmbH

LDHB antibody

LDHB Rabbit IgG Aff - Purified Bov Dot, E, ICC/IF, ID, IP, R, WB
1 mg / €430.00
  Acris Antibodies GmbH

LDHB antibody

LDHB Rabbit IgG Azide Free Por Dot, E, ICC/IF, ID, IP, R, WB
10 mg / €230.00
  Acris Antibodies GmbH

LDHB antibody

LDHB Rabbit IgG Biotin Por Dot, E, ICC/IF, ID, IP, R, WB
1 ml / €340.00
  Acris Antibodies GmbH

LDHB antibody

LDHB Rabbit IgG Aff - Purified Por Dot, E, ICC/IF, ID, IP, R, WB
1 mg / €430.00
  Acris Antibodies GmbH

LDHB (Isoenzyme H4) antibody

LDHB Sheep IgG Aff - Purified Rb Dot, E, ICC/IF, ID, IP, R, WB
1 mg / €430.00
  Acris Antibodies GmbH

LDHB antibody

Detection limit for recombinant GST tagged LDHB is approximately 0.3ng/ml as a capture antibody. Mouse IgG1 2F11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

LDHB antibody

Immunoblot analysis of cell lysates.
Lane 1 : HeLa cell lysate.
 Lnae 2 : Ramos cell lysate.
Lane 3 : A-431 cell lysate.
Lane 4 : Jurkat cell lysate.
Lane 5 : K-562 cell lysate. Mouse AF21F4 Hu, Ms, Rt E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

LDHB antibody

Immunoblot analysis of cell lysates.
Lane 1 : HeLa cell lysate.
 Lnae 2 : RD cell lysate.
Lane 3 : Ramos cell lysate.
Lane 4 : A-431 cell lysate.
Lane 5 : Jurkat cell lysate.
Lane 6 : K-562 cell lysate.
Lane 7 : C6 cell lysate. Mouse 60H11 Hu, Ms, Rt E, IP, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

LDHB antibody

Immunohistochemistry: LDH-B (Heart Subunit) Antibody (EP1566Y) [NB110-57160] - Immunohistochemical analysis of paraffin-embedded human liver carcinoma using anti-LDH-B (Cat # 110-57160). Rabbit IgG EP1566Y Supernatant Hu, Ms, Rt F, ICC/IF, IP, P, WB
0.1 ml / €490.00
  Novus Biologicals Inc.

LDHB antibody

Western Blot: Lactate Dehydrogenase B Antibody [NBP1-31945-(30 ug of whole cell lysate) A: A549 B: Hela 10% SDS PAGE  diluted at 1:1000 Rabbit IgG Hu ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

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