LEF1 antibody

Principal name

LEF1 antibody

Alternative names for LEF1 antibody

Lymphoid enhancer-binding factor 1, LEF-1, T cell-specific transcription factor 1-alpha, TCF1-alpha

SwissProt ID

P27782 (Mouse), Q9QXN1 (Rat), Q9UJU2 (Human)

Gene ID

51176 (LEF1)

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Por (Porcine), Rb (Rabbit), Can (Canine), Chk (Chicken), Rt (Rat), Ze (Zebrafish), African clawed frog

Available hosts

Rabbit, Mouse, Goat, Sheep

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of LEF1 antibody

The human T cell-specific transcription factor TCF-1 alpha plays a key role in the tissue-specific activation of the T cell receptor (TCR) C alpha enhancer and binds to pyrimidine-rich elements (5'-PyCTTTG-3') present in a variety of other T cell-specific control regions. TCF-1 alpha is currently unique among the newly emerging family of DNA-binding regulatory proteins that share the HMG motif in that it is a highly tissue-specific RNA polymerase II transcription factor (1). TCF-1 alpha participates in the regulation of TCR alpha enhancer by facilitating the assembly of multiple proteins into a higher order nucleoprotein complex. The function of LEF-1 is dependent, in part, on the HMG domain that induces a sharp bend in the DNA helix, and on an activation domain that stimulates transcription only in a specific context of other enhancer-binding proteins (2). Constitutive activation of the Wnt signaling pathway is a root cause of many colon cancers. Activation of this pathway is caused by genetic mutations that stabilize the beta-catenin protein, allowing it to accumulate in the nucleus and form complexes with any member of the lymphoid enhancer factor and T-cell factor family of transcription factors (referred to collectively as LEF/TCFs) to activate transcription of target genes (3).

General readings

Gutierrez, A. Jr., et al. Blood 116(16):2975-2983(2010)
Kalsi, G., et al. Hum. Mol. Genet. 19(12):2497-2506(2010)
Chen, Q.Y., et al. J. Immunol. 184(9):5047-5054(2010)
Beagle, B., et al. PLoS ONE 5 (7), E11821 (2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

LEF1 (all isoforms) antibody

LEF1 Mouse IgG1 1C3.1D10 Purified Hu WB
0.1 mg / €370.00
  Acris Antibodies GmbH

LEF1 antibody

LEF1 Sheep IgG Purified Hu WB
0.1 mg / €360.00
  Acris Antibodies GmbH

LEF1 antibody

Western blot analysis of LEF1 over-expressed 293 cell line, cotransfected with LEF1 Validated Chimera RNAi ( Cat # H00051176-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with LEF1 monoclonal antibody (M01), clone 3H5 (Cat # H00051176-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. Mouse IgG1 3H5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

LEF1 antibody

Immunofluorescence of monoclonal antibody to LEF1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG1 5A3 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

LEF1 antibody

TA303096 (1µg/ml) staining of Daudi cell lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
20 µg / €200.00
  Acris Antibodies GmbH

LEF1 antibody

TA303096 (1µg/ml) staining of Daudi cell lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

LEF1 (N-term) antibody

LEF1 Antibody (N-term) (Cat. #TA324467) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the LEF1 antibody detected the LEF1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

LEF1 antibody

WB Suggested Anti-LEF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Hela cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

LEF1 antibody

WB Suggested Anti-LEF1 Antibody Titration: 2.0 ug/ml; Positive Control: Jurkat cell lysateLEF1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

LEF1 antibody

WB Suggested Anti-LEF1 Antibody Titration: 0.1-2.0ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

LEF1 antibody

Detection of endogenous LEF Whole cell lysates of serum starved Jurkat were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab 2D12 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Mouse IgG1 2D12 Purified Hu E, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

LEF1 antibody

Immunofluorescence staining of Cos-1 cells transfected with (top to bottom) full length hLEF-1, a LEF-1 mutant missing the nuclear localization signal, TCF-4 and TCF-1. Cells were stained with LEF-1 Antibody Cat.-No AM05260PU-L (Clone REMB1) and visualized with FITC conjugated mouse IgG. DAPI was used to detect all nuclei. The LEF-1 clone REMB1 recognized an epitope in the beta catenin binding domain that is present only in LEF-1. Mouse IgG1 REMB1 Purified Hu, Ms ICC/IF, P, WB
0.25 mg / €540.00
  Acris Antibodies GmbH

LEF1 (HMG Binding Domain) antibody

Immunofluorescence staining of Cos-1 cells transfected with (from Top Down) LEF-1, LEF-1 mutant missing nuclear localization signal, TCF-4 and TCF-1. 
Left column shows AM05261PU-N HMG/TCF antibody, (Clone REMB6) visualized with FITC anti-Mouse. 
Right column is DAPI staining to detect all nuclei. Mouse IgG1 REMB6 Purified Hu, Ms ICC/IF, WB
0.25 mg / €540.00
  Acris Antibodies GmbH

LEF1 (HMG Binding Domain) antibody

Immunofluorescence staining of Cos-1 cells transfected with (from Top Down) LEF-1, LEF-1 mutant missing nuclear localization signal, TCF-4 and TCF-1. 
Left column shows AM05261PU-N HMG/TCF antibody, (Clone REMB6) visualized with FITC anti-Mouse. 
Right column is DAPI staining to detect all nuclei. Mouse IgG1 REMB6 Purified Hu, Ms ICC/IF, WB
0.1 mg / €360.00
  Acris Antibodies GmbH

LEF1 (236-242) antibody

LEF1 Mouse IgG1 3A12 Purified Hu WB
0.1 mg / €340.00
  Acris Antibodies GmbH

LEF1 (alternate exon) antibody

Figure 1. Western blot analysis using LEF-1 (alternate exon) antibody (AM05275PU-N) on Cos cells transfected with the indicated proteins. Mouse IgG1 1C3 Purified Hu WB
0.1 mg / €370.00
  Acris Antibodies GmbH

LEF1 antibody

Western Blot analysis of LEF1 expression in transfected 293T cell line (H00051176-T01) by LEF1 MaxPab polyclonal antibody.

Lane 1: LEF1 transfected lysate(43.89 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

LEF1 antibody

Detection limit for recombinant GST tagged LEF1 is approximately 0.1ng/ml as a capture antibody. Mouse IgG2a 6H3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

LEF1 antibody

Immunofluorescence of monoclonal antibody to LEF1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG1 2C9 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

LEF1 antibody

LEF1 monoclonal antibody (M05), clone 1H2 Western Blot analysis of LEF1 expression in Jurkat ( Cat # L017V1 ). Mouse IgG1 1H2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

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