LEF1 antibody

Principal name

LEF1 antibody

Alternative names for LEF1 antibody

Lymphoid enhancer-binding factor 1, LEF-1, T cell-specific transcription factor 1-alpha, TCF1-alpha

SwissProt ID

P27782 (Mouse), Q9QXN1 (Rat), Q9UJU2 (Human)

Gene ID

51176 (LEF1), 16842 (Lef1), 161452

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Por (Porcine), Rb (Rabbit), Can (Canine), Chk (Chicken), Rt (Rat), Ze (Zebrafish), African clawed frog

Available hosts

Rabbit, Mouse, Sheep, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of LEF1 antibody

Lymphoid Enhancer Factor -1 is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF-1, TCF-1, TCF-3 and TCF-4). These factors play a crucial role in WNT/WIngless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer.LEF-1 is expressed during development in many different differentiating tissues, and in a few tissues after birth. LEF-1 expression is required for proper development of breast, teeth, hair, whiskers and the trigeminal nerve. It is redundant with TCF-1 (for T Cell Factor-1) for correct development of T lymphocytes in the thymus. LEF-1 is a 399 amino acid protein that appears as a cluster of [polypeptides ranging in size between 55-57 kD on SDS-PAGE analysis. Alternative splice forms of LEF-1 and other LEF/TCF family members have been identified, but the expression pattern and relative abundance of these alternative forms have not been well studied. They can appear on westerns as smaller immunoreactive forms.

General readings

Gutierrez, A. Jr., et al. Blood 116(16):2975-2983(2010)
Kalsi, G., et al. Hum. Mol. Genet. 19(12):2497-2506(2010)
Chen, Q.Y., et al. J. Immunol. 184(9):5047-5054(2010)
Beagle, B., et al. PLoS ONE 5 (7), E11821 (2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

LEF1 (all isoforms) antibody

LEF1 Mouse IgG1 1C3.1D10 Purified Hu WB
0.1 mg / €380.00
  Acris Antibodies GmbH

LEF1 antibody

LEF1 Sheep IgG Purified Hu WB
0.1 mg / €370.00
  Acris Antibodies GmbH

LEF1 antibody

Western blot analysis of LEF1 over-expressed 293 cell line, cotransfected with LEF1 Validated Chimera RNAi ( Cat # H00051176-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with LEF1 monoclonal antibody (M01), clone 3H5 (Cat # H00051176-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. Mouse IgG1 3H5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

LEF1 antibody

Immunofluorescence of monoclonal antibody to LEF1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG1 5A3 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

LEF1 antibody

TA303096 (1µg/ml) staining of Daudi cell lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

LEF1 (N-term) antibody

LEF1 Antibody (N-term) (Cat. #TA324467) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the LEF1 antibody detected the LEF1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

LEF1 antibody

WB Suggested Anti-LEF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Hela cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

LEF1 antibody

WB Suggested Anti-LEF1 Antibody Titration: 2.0 ug/ml; Positive Control: Jurkat cell lysateLEF1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

LEF1 antibody

WB Suggested Anti-LEF1 Antibody Titration: 0.1-2.0ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

LEF1 antibody

The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using (LEF1 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

LEF1 antibody

Jurkat cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-LEF1 (clone 15H5A18) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection. Mouse 15H5A18 Purified Hu WB
0.1 mg / €280.00
  OriGene Technologies, Inc.

LEF1 antibody

Detection of endogenous LEF Whole cell lysates of serum starved Jurkat were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab 2D12 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Mouse IgG1 2D12 Purified Hu E, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

LEF1 antibody

Immunofluorescence staining of Cos-1 cells transfected with (top to bottom) full length hLEF-1, a LEF-1 mutant missing the nuclear localization signal, TCF-4 and TCF-1. Cells were stained with LEF-1 Antibody Cat.-No AM05260PU-L (Clone REMB1) and visualized with FITC conjugated mouse IgG. DAPI was used to detect all nuclei. The LEF-1 clone REMB1 recognized an epitope in the beta catenin binding domain that is present only in LEF-1. Mouse IgG1 REMB1 Purified Hu, Ms ICC/IF, P, WB
0.25 mg / €550.00
  Acris Antibodies GmbH

LEF1 (HMG Binding Domain) antibody

Immunofluorescence staining of Cos-1 cells transfected with (from Top Down) LEF-1, LEF-1 mutant missing nuclear localization signal, TCF-4 and TCF-1. 
Left column shows AM05261PU-N HMG/TCF antibody, (Clone REMB6) visualized with FITC anti-Mouse. 
Right column is DAPI staining to detect all nuclei. Mouse IgG1 REMB6 Purified Hu, Ms ICC/IF, WB
0.25 mg / €550.00
  Acris Antibodies GmbH

LEF1 (HMG Binding Domain) antibody

Immunofluorescence staining of Cos-1 cells transfected with (from Top Down) LEF-1, LEF-1 mutant missing nuclear localization signal, TCF-4 and TCF-1. 
Left column shows AM05261PU-N HMG/TCF antibody, (Clone REMB6) visualized with FITC anti-Mouse. 
Right column is DAPI staining to detect all nuclei. Mouse IgG1 REMB6 Purified Hu, Ms ICC/IF, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

LEF1 (236-242) antibody

LEF1 Mouse IgG1 3A12 Purified Hu WB
0.1 mg / €350.00
  Acris Antibodies GmbH

LEF1 (alternate exon) antibody

Figure 1. Western blot analysis using LEF-1 (alternate exon) antibody (AM05275PU-N) on Cos cells transfected with the indicated proteins. Mouse IgG1 1C3 Purified Hu WB
0.1 mg / €380.00
  Acris Antibodies GmbH

LEF1 antibody

Western Blot analysis of LEF1 expression in transfected 293T cell line (H00051176-T01) by LEF1 MaxPab polyclonal antibody.

Lane 1: LEF1 transfected lysate(43.89 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

LEF1 antibody

Detection limit for recombinant GST tagged LEF1 is approximately 0.1ng/ml as a capture antibody. Mouse IgG2a 6H3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

LEF1 antibody

Immunofluorescence of monoclonal antibody to LEF1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG1 2C9 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

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