LEFTY2 antibody

Principal name

LEFTY2 antibody

Alternative names for LEFTY2 antibody

LEFTY2, EBAF, LEFTA, LEFTYA, TGFB4, Left-right determination factor 2, Protein lefty-2, Left-right determination factor A, Protein lefty-A, Transforming growth factor beta-4, TGF-beta-4, Endometrial bleeding-associated factor

SwissProt ID

O00292 (Human), P57785 (Mouse)

Gene ID

7044 (LEFTY2), 320202 (Lefty2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Por (Porcine), African clawed frog

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of LEFTY2 antibody

During vertebrate embryogenesis, a left-right axis is established. Secreted growth factors of the TGF-beta family, including gene products derived from nodal, lefty-1 and lefty-2, play crucial roles in establishing left-right asymmetries. TGF-beta (Transforming growth factor-beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF-beta actions are directed by ligand-induced activation of TGF-beta receptors. Complexes formed move into the nucleus, where they act as components of a transcriptional complex. Lefty, a novel member of the TGF-beta superfamily, inhibits TGF-beta signaling. Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF-beta receptor. Lefty also inhibits events downstream from R-Smad phosphorylation. Lefty provides a repressed state of TGF-beta-responsive genes. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans. Members of the TGF-beta superfamily require processing for their activation. Cleavage is therefore an essential step for Lefty activation. Lefty is synthesized as a large inactive precursor (42 Kda) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28kDa form induces MAPK activity.

General readings

Cornet,P.B., (2005) J. Clin. Endocrinol. Metab. 90 (2), 1001-1011

14 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

LEFTY2 antibody

Sample (30 ug of whole cell lysate). A: A431 . 10% SDS PAGE. TA308258 diluted at 1:1000. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

LEFTY2 antibody

Mab anti-Human LEFTY antibody to detect by WB partially purified recombinant 6X His tagged human LEFTY. The blot was incubated with a 1:2,000 dilution of Mab anti-Human LEFTY at RT for 30 min followed by detection using IRDye™800 labeled Goat-a-Mouse IgG [H&L] diluted 1:1,000. Lane 1 contains an unrelated 6X His tagged protein and shows that the antibody does not recognize the epitope tag. Lane 2 contains partially purified recombinant human LEFTY. Mouse IgG2a 7C5G1H6H10 Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

LEFTY2 antibody

Western Blot analysis of LEFTY2 expression in transfected 293T cell line (H00007044-T01) by LEFTY2 MaxPab polyclonal antibody.

Lane 1: LEFTY2 transfected lysate(40.26 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

LEFTY2 antibody

LEFTY2 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

LEFTY2 antibody

Immunoprecipitation of LEFTY2 transfected lysate using anti-LEFTY2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with LEFTY2 MaxPab mouse polyclonal antibody (B01) (H00007044-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

LEFTY2 antibody

Western Blot analysis of LEFTY2 expression in transfected 293T cell line (H00007044-T01) by LEFTY2 MaxPab polyclonal antibody.

Lane 1: LEFTY2 transfected lysate(40.90 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

LEFTY2 antibody

LEFTY2 Mouse IgG2a 1D10 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

LEFTY2 antibody

LEFTY2 monoclonal antibody (M04), clone 1H6. Western Blot analysis of LEFTY2 expression in human Skeletal muscle. Mouse IgG2b 1H6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

LEFTY2 antibody

LEFTY2 monoclonal antibody, clone 7C5G1H6H10 ( Cat # MAB2032 ) is shown to detect by western blot partially purified recombinant 6X His tagged human LEFTY2.
Detection occurs after 1.0 µg of protein is loaded in each lane.
The blot was incubated with a 1:2,000 dilution of LEFTY2 monoclonal antibody, clone 7C5G1H6H10 at room temperature for 30 min followed by detection using IRDyeTM800 labeled Goat-a-Mouse IgG [H&L] diluted 1 : 1,000.
Lane 1 contains an unrelated 6X His tagged protein and shows that the antibody does not recognize the epitope tag.
Lane 2 contains partially purified recombinant human LEFTY.
The antibody may be used to detect endogenous human LEFTY. Hu, Ms, Rt
0.1 mg / €560.00
  Abnova Taiwan Corp.

LEFTY2 antibody

Human Uterus; Immunohistochemistry with Uterus tissue at an antibody concentration of 5ug/ml using anti-LEFTY2 antibody (ARP42107_P050); LEFTY2 antibody - N-terminal region (ARP42107_P050) in Human Uterus cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Hu, Ms, Por, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

LEFTY2

LEFTY2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

LEFTY2

LEFTY2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Antibody Pairs

Catalog No.    

LEFTY2 (Human) IP-WB Antibody Pair

LEFTY2 (Human) IP-WB Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

LEFTY2 293T Cell Transient Overexpression Lysate(Denatured)

LEFTY2 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

14 Item(s)

per page
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