LEO1 antibody

Principal name

LEO1 antibody

Alternative names for LEO1 antibody

LEO-1, RDL, RNA polymerase-associated protein LEO1, Replicative senescence down-regulated leo1-like protein

SwissProt ID

Q5XJE5 (Mouse), Q641X2 (Rat), Q8WVC0 (Human)

Gene ID

123169 (LEO1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunoprecipitation (IP), ChIP assay (ChIP), Dot blot (Dot)

Background of LEO1 antibody

LEO1 is a component of the PAF/RNA polymerase II transcription complex. It interacts with parafibromin, PAF1 and CTR9. This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

LEO1 (N-term) antibody

Western blot analysis of anti-Leo1 Antibody (N-term) (Cat. #TA300199) in HL60 cell line lysates (35ug/lane). Leo1(arrow) was detected using the purified Pab. Rabbit Ig Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

LEO1 antibody

Host: Rabbit; Target Name: LEO1; Sample Tissue: HepG2 Whole cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

LEO1 antibody

WB using the antibody against hLeo1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. Rabbit IgG Purified Hu
0.1 ml / €325.00
  OriGene Technologies, Inc.

LEO1 antibody

WB using the antibody against hLeo1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. A MW marker is shown on the left, the location of the protein of interest is shown on the right. Rabbit IgG Purified Hu
50 µg / €325.00
  OriGene Technologies, Inc.

LEO1 pSer551 antibody

Dot blot analysis of anti-Phospho-Leo1 pSer551 Antibody (Cat.#AP12974PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Ig Aff - Purified Hu
0.4 ml / €370.00
  Acris Antibodies GmbH

LEO1 pSer10 antibody

Dot blot analysis of anti-Phospho-LEO1 pSer10 Antibody (Cat.#AP12991PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Ig Aff - Purified Hu
0.4 ml / €370.00
  Acris Antibodies GmbH

LEO1 pSer10 antibody

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Hu E, P
50 µg / €470.00
  Acris Antibodies GmbH

LEO1 antibody

LEO1 (phospho S551) polyclonal antibody ( Cat # PAB8172 ). Western blot analysis of LEO1 expression in Jurkat. Rabbit Hu Dot, E, WB
0.4 ml / €400.00
  Abnova Taiwan Corp.

LEO1 antibody

HeLa cell lysate was analysed by Western blot using LEO1 polyclonal antibody ( Cat # PAB14102 ), used at dilution 1 : 2000. Size and location of the protein are indicated. Rabbit Hu ChIP, WB
50 µg / €390.00
  Abnova Taiwan Corp.

LEO1 antibody

Western Blot: Leo1 Antibody [NB100-79777] - Detection of Human Leo1 by Western Blot. Samples: Whole  cell lysate (WCL; 10, 30 or 50 mcg) from 293T cells (E) or 293T  cells that were transfected with a Leo1 expression construct (T).  Antibody: Affinity purified rabbit anti-Leo1 antibody (Cat. No.  Nb100-79777) used at 1 mcg/ml. Detection: Chemiluminescence  with an exposure time of 1 minute. Rabbit IgG Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

LEO1 antibody

Western Blot and Immunoprecipitation: Leo-1 Antibody [NB600-276] - Detection of Human Leo1on HeLa whole cell lysate using NB600-276. In B, Leo1 was immunoprecipitated by using NB600-276 at 3 mcg/10mg with or without blocking peptide. Rabbit Aff - Purified Hu, Ms ICC/IF, IP, P, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

LEO1 antibody

LEO1 Rabbit Purified Hu E, P
50 µg / €480.00
  Novus Biologicals Inc.

LEO1 antibody

Immunohistochemistry-Paraffin: LEO1 Antibody [NBP1-86236] - Immunohistochemical staining of human rectum shows moderate nuclear and cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

LEO1 antibody

Immunohistochemistry-Paraffin: LEO1 Antibody [NBP1-89423] - Immunohistochemical staining of human rectum shows nuclear and cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

LEO1

LEO1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

LEO1

LEO1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

LEO1

LEO1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

17 Item(s)

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