Leptin antibody

Principal name

Leptin antibody

Alternative names for Leptin antibody

LEP, OB, OBS, Obesity factor, Obese protein

SwissProt ID

O02720 (Canfa), O42164 (Chick), P41159 (Human), P41160 (Mouse), P50595 (Bovin), P50596 (Rat), Q28603 (Sheep), Q29406 (Pig), Q8WML2 (Rabit), Q9N2C1 (Felca), Q9TU09 (Horse)

Gene ID

3952 (LEP), 25608 (Lep), 16846

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Sh (Sheep), Eq (Equine), Por (Porcine)

Available hosts

Rabbit, Mouse, Goat, Sheep, Chicken

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), ELISA (capture) (E(capture)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Frozen Sections (C), Immunoprecipitation (IP), Radioimmunoassay (R), Neutralisation (NEUT)

Background of Leptin antibody

LEP is a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development.This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

1. Ricci MR, Lee MJ, Russell CD, Wang Y, Sullivan S, Schneider SH, Brolin RE, Fried SK . Isoproterenol decreases leptin release from rat and human adipose tissue through posttranscriptional mechanisms. Am J Physiol Endocrinol Metab . Apr;288(4):E798-804 (2005)
See: http://ajpendo.physiology.org/cgi/content/full/288/4/E798
2. Ricci M.R., Lee M.J., et al. Isoproterenol decreases leptin release from rat and human adipose tissue through posttranscriptional mechanisms. Am. J. Physiol. Endocrinol. Metab. 2005; (288), E798-E804.
3. Maffei M., Halaas J., Ravussin E. et al. Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight reduced subjects. Nature Med. 1995; 1155 1161.
4. Maffei M., Halaas J., Ravussin E. et al. Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects. Nature Med. 1, 1155-1161 (1995) .
5. Lonnqvist F., Arner P., Nordfors L. and Shalling M. Overexpression of the obese (ob) gene in adipose tissue of human subjects. Nature Med. 1, 950-953 (1995) .
6. Zhang Y., Proenca R., Maffei M., Barone M., Leopold L., Friedman J. Positional Cloning of the Mouse Obese Gene and its Human Homologue. Nature 372, 425-432 (1994).
7. Considine R.V., Sinha M.K., Heiman M.L. et al. Serum immunoreactive leptin concentrations in normal weight and obese humans. N. Engl. J. Med. 1996; (334), 292 295.
8. Considine R.V., Sinha M.K., Heiman M.L. et al. Serum immunoreactive leptin concentrations in normal-weight and obese humans. N. Engl. J. Med. 334, 292-295 (1996).
9. Halaas J.L., Gajiwala K.S., Maffei M. et al. Weight reducing effects of the plasma protein encoded by the obese gene. Science 1995; (269), 543 546.
10. Halaas J.L., Gajiwala K.S., Maffei M. et al. Weight-reducing effects of the plasma protein encoded by the obese gene. Science 269, 543-546 (1995).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Leptin (N-term) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue reacted with Leptin Antibody (N-term) Cat.-No AP52465PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Aff - Purified Hu F, ICC/IF, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Leptin antibody

Western blot analysis using recombinant rat Leptin. Lane 1: 5 ng, Lane 2: 10 ng. Rabbit Purified Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Leptin antibody

Predicted cell location: Cytoplasm. Positive control: Human thyroid and liver cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using LEP antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Leptin antibody

Predicted cell location: Cytoplasm. Positive control: Human thyroid and cervical cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using LEP antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Leptin antibody

To detect hLeptin by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hLeptin. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Leptin antibody

To detect hLeptin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Leptin (TA328366) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hLeptin. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Leptin antibody

To detect mLeptin by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mLeptin. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Leptin antibody

To detect mLeptin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine Leptin (TA328528) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mLeptin. Rabbit IgG Purified E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Leptin antibody

Western blot analysis of extracts of various cell lines, using LEP antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Leptin antibody

Indirect ELISA assay for anti-Leptin mouse mAb.Antigen coating concentration: 4ug/ml. Mouse IgG2a 5C11-G6-B12 Purified
0.1 ml / €325.00
  OriGene Technologies, Inc.

Leptin antibody

Indirect ELISA assay for anti-Leptin mouse mAb.Antigen coating concentration: 4ug/ml. Mouse IgG1 7E3-D11-B7 Purified
0.1 ml / €325.00
  OriGene Technologies, Inc.

Leptin antibody

Leptin Mouse IgG1 Azide Free Hu E, WB
0.5 mg / €220.00
  Acris Antibodies GmbH

Leptin antibody

Leptin Goat Biotin Rt E, WB
50 µg / €340.00
  Acris Antibodies GmbH

Leptin antibody

Leptin Goat Biotin Rt E, WB
25 µg / €230.00
  Acris Antibodies GmbH

Leptin antibody

Leptin Goat Aff - Purified Rt E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Leptin antibody

Leptin Goat Aff - Purified Rt E, WB
50 µg / €230.00
  Acris Antibodies GmbH

Leptin antibody

Leptin Sheep IgG Purified Hu, Ms, Rt E
1 ml / €430.00
  Acris Antibodies GmbH

Leptin antibody

Breast: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Serum Hu, Ms E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Leptin antibody

Leptin Rabbit IgG Ig Fraction Ms E, WB
1 mg / €1,150.00
  Acris Antibodies GmbH

Leptin (C-term) antibody

Confocal immunofluorescent analysis of Leptin Antibody (C-term) Cat.-No AP13112PU-N with HepG2 cell followed by Alexa Fluor®488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). Rabbit Ig Purified Hu ICC/IF, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

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