Leptin antibody

Principal name

Leptin antibody

Alternative names for Leptin antibody

LEP, OB, OBS, Obesity factor, Obese protein

SwissProt ID

O02720 (Canfa), O42164 (Chick), P41159 (Human), P41160 (Mouse), P50595 (Bovin), P50596 (Rat), Q28603 (Sheep), Q29406 (Pig), Q8WML2 (Rabit), Q9N2C1 (Felca), Q9TU09 (Horse)

Gene ID

3952 (LEP), 25608 (Lep), 16846

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse)

Available hosts

Mouse, Goat, Sheep, Rabbit

Available applications

Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), ELISA (capture) (E(capture)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Frozen Sections (C), Immunoprecipitation (IP), Radioimmunoassay (R)

Background of Leptin antibody

Leptin, the product of the ob (obese) gene, is a single-chain 16 kDa protein consisting of 146 amino acid residues. Leptin is produced mainly in the adipose tissue, and is considered to play an important role in appetite control, fat metabolism and body weight regulation. It targets the central nervous system, particularly hypothalamus, affecting food intake. The primary effect of leptin appears to be mediated by leptin receptors expressed mainly in the hypothalamus. In humans, leptin levels correlate with body mass index (BMI) and percentage body fat, and are elevated even in obese individuals. Leptin has a dual action; it decreases the appetite and increases energy consumption, causing more fat to be burned. Leptin is secreted in circadian fashion with nocturnal rise in both lean and obese patients. Mutations of the ob gene resulting in leptin deficiency are the cause of obesity in the ob/ob mice. Endogeneous leptin can normalize their body weight. In contrast, high levels of leptin in obese human subjects point to an insensitivity to endogeneous leptin. Other factors in addition to the amount of body fat appear to regulate leptin action: insulin, glucocorticoids, catecholamines and sex hormones. Studies have shown that leptin may be linked to reproductive function.

General readings

1. Ricci MR, Lee MJ, Russell CD, Wang Y, Sullivan S, Schneider SH, Brolin RE, Fried SK . Isoproterenol decreases leptin release from rat and human adipose tissue through posttranscriptional mechanisms. Am J Physiol Endocrinol Metab . Apr;288(4):E798-804 (2005)
See: http://ajpendo.physiology.org/cgi/content/full/288/4/E798
2. Ricci M.R., Lee M.J., et al. Isoproterenol decreases leptin release from rat and human adipose tissue through posttranscriptional mechanisms. Am. J. Physiol. Endocrinol. Metab. 2005; (288), E798-E804.
3. Maffei M., Halaas J., Ravussin E. et al. Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight reduced subjects. Nature Med. 1995; 1155 1161.
4. Maffei M., Halaas J., Ravussin E. et al. Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects. Nature Med. 1, 1155-1161 (1995) .
5. Lonnqvist F., Arner P., Nordfors L. and Shalling M. Overexpression of the obese (ob) gene in adipose tissue of human subjects. Nature Med. 1, 950-953 (1995) .
6. Zhang Y., Proenca R., Maffei M., Barone M., Leopold L., Friedman J. Positional Cloning of the Mouse Obese Gene and its Human Homologue. Nature 372, 425-432 (1994).
7. Considine R.V., Sinha M.K., Heiman M.L. et al. Serum immunoreactive leptin concentrations in normal weight and obese humans. N. Engl. J. Med. 1996; (334), 292 295.
8. Considine R.V., Sinha M.K., Heiman M.L. et al. Serum immunoreactive leptin concentrations in normal-weight and obese humans. N. Engl. J. Med. 334, 292-295 (1996).
9. Halaas J.L., Gajiwala K.S., Maffei M. et al. Weight reducing effects of the plasma protein encoded by the obese gene. Science 1995; (269), 543 546.
10. Halaas J.L., Gajiwala K.S., Maffei M. et al. Weight-reducing effects of the plasma protein encoded by the obese gene. Science 269, 543-546 (1995).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Leptin (N-term) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue reacted with Leptin Antibody (N-term) Cat.-No AP52465PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Aff - Purified Hu F, ICC/IF, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Leptin antibody

  Rabbit polyclonal anti-Leptin antibody  
Western blot analysis using recombinant rat Leptin. Lane 1: 5 ng, Lane 2: 10 ng. Rabbit Purified Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Leptin antibody

  Anti-LEP Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human thyroid and liver cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using LEP antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Leptin antibody

  Anti-LEP Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human thyroid and cervical cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using LEP antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Leptin antibody

  Biotinylated Anti-Human Leptin Rabbit Polyclonal Antibody  
To detect hLeptin by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hLeptin. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Leptin antibody

  Anti-Human Leptin Rabbit Polyclonal Antibody  
To detect hLeptin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Leptin (TA328366) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hLeptin. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Leptin antibody

  Biotinylated Anti-Murine Leptin Rabbit Polyclonal Antibody  
To detect mLeptin by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mLeptin. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Leptin antibody

  Anti-Murine Leptin Rabbit Polyclonal Antibody  
To detect mLeptin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine Leptin (TA328528) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mLeptin. Rabbit IgG Purified E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Leptin antibody

  Rabbit anti-LEP Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using LEP antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Leptin antibody

  Mouse Monoclonal Leptin Antibody  
Indirect ELISA assay for anti-Leptin mouse mAb.Antigen coating concentration: 4ug/ml. Mouse IgG2a 5C11-G6-B12 Purified
0.1 ml / €325.00
  OriGene Technologies, Inc.

Leptin antibody

  Mouse Monoclonal Leptin Antibody  
Indirect ELISA assay for anti-Leptin mouse mAb.Antigen coating concentration: 4ug/ml. Mouse IgG1 7E3-D11-B7 Purified
0.1 ml / €325.00
  OriGene Technologies, Inc.

Leptin antibody

  Rabbit Polyclonal Anti-LEP Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (LEP Antibody) at dilution 1/35, on the right is treated with fusion protein. (Original magnification: ×200) Rabbit IgG Purified Hu E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Leptin antibody

Leptin Mouse IgG1 Azide Free Hu E, WB
0.5 mg / €220.00
  Acris Antibodies GmbH

Leptin antibody

Leptin Goat Biotin Rt E, WB
50 µg / €340.00
  Acris Antibodies GmbH

Leptin antibody

Leptin Goat Biotin Rt E, WB
25 µg / €230.00
  Acris Antibodies GmbH

Leptin antibody

Leptin Goat Aff - Purified Rt E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Leptin antibody

Leptin Goat Aff - Purified Rt E, WB
50 µg / €230.00
  Acris Antibodies GmbH

Leptin antibody

Leptin Sheep IgG Purified Hu, Ms, Rt E
1 ml / €430.00
  Acris Antibodies GmbH

Leptin antibody

Breast: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Serum Hu, Ms E, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

Leptin antibody

Leptin Rabbit IgG Ig Fraction Ms E, WB
1 mg / €1,180.00
  Acris Antibodies GmbH

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