LGR4 / GPR48 antibody

Principal name

LGR4 / GPR48 antibody

Alternative names for LGR4 / GPR48 antibody

G-protein coupled receptor 48, Leucine-rich repeat-containing G-protein coupled receptor 4

SwissProt ID

A2ARI4 (Mouse), Q9BXB1 (Human), Q9Z2H4 (Rat)

Gene ID

55366 (LGR4), 107515 (Lgr4), 286994

Available reactivities

Chimp (Chimpanzee), Hu (Human), Macaque, Orang-Utan, Ms (Mouse), Bov (Bovine), Can (Canine), Mky (Monkey), Por (Porcine), Prim (Primate), Rb (Rabbit), Rt (Rat), Bear, Eq (Equine), Hst (Hamster), Op (Opossum), Xen (Xenopus), Tr (Turkey), Bt (Bat), Chk (Chicken)

Available hosts

Mouse, Rabbit, Rat

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Flow Cytometry (F)

Background of LGR4 / GPR48 antibody

Kit Component:
- KN309256G1, Lgr4 gRNA vector 1 in pCas-Guide vector
- KN309256G2, Lgr4 gRNA vector 2 in pCas-Guide vector
- KN309256D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

1. Weng,J., Luo,J., Cheng,X., Jin,C., Zhou,X., Qu,J., Tu,L., Ai,D.,Li,D., Wang,J., Martin,J.F., Amendt,B.A. and Liu,M.(2008) Deletion of G proteincoupled
receptor 48 leads to ocular anterior segment dysgenesis (ASD) through down-regulation of Pitx2. Proc. Natl. Acad. Sci. U.S.A. 105 (16), 6081-6086.
2. Gao,Y., Kitagawa,K., Hiramatsu,Y., Kikuchi,H., Isobe,T., Shimada,M., Uchida,C., Hattori,T., Oda,T., Nakayama,K., Nakayama,K.I., Tanaka,T., Konno,H. and Kitagawa,M. (2006) Up-regulation of GPR48 induced by down-regulation of p27Kip1 enhances carcinoma cell invasiveness and metastasis. Cancer Res. 66 (24), 11623-11631.
3. Loh,E.D., Broussard,S.R. and Kolakowski,L.F. (2001) Molecular characterization of a novel glycoprotein hormone G-protein-coupled receptor. Biochem. Biophys. Res. Commun. 282 (3), 757-764.
4. Loh,E.D., Broussard,S.R., Liu,Q., Copeland,N.G., Gilbert,D.J., Jenkins,N.A. and Kolakowski,L.F. Jr. (2000) Chromosomal localization of GPR48, a novel glycoprotein hormone receptor like GPCR, in human and mouse with radiation hybrid and interspecific backcross mapping. Cytogenet. Cell Genet. 89 (1-2), 2-5.
5. Hsu,S.Y., Liang,S.G. and Hsueh,A.J. (1998) Characterization of two LGR genes homologous to gonadotropin and thyrotropin receptors with extracellular leucine-rich repeats and a G protein-coupled, seven-transmembrane region. Mol. Endocrinol. 12 (12), 1830-1845.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

LGR4 / GPR48 antibody

  LGR4 mouse monoclonal antibody, clone OTI2D1 (formerly 2D1)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGR4 (RC221345, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGR4. Mouse IgG1 OTI2D1 Purified Hu F, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

LGR4 / GPR48 antibody

  LGR4 mouse monoclonal antibody, clone OTI5B8 (formerly 5B8)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGR4 (RC221345, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGR4. Mouse IgG1 OTI5B8 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

LGR4 / GPR48 antibody

  Rabbit polyclonal anti-LGR4 antibody  
Western blot analysis of extracts from Jurkat cells, using LGR4 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

LGR4 / GPR48 antibody

  Mouse monoclonal anti-LGR4 antibody  
Anti-LGR4 monoclonal antibody was used diluted to 5 ug/ml to detect LGR4 staining at the membrane of cells in various human tissues. A. Brain cerebellum. B. Pancreas islet. Mouse IgG2b 6G8.B3.G5.C3 Purified Chimp, Hu, Macaque, Orang-Utan E, P, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

LGR4 / GPR48 (N-term, extracell. dom.) antibody

  Rabbit Polyclonal Anti-LGR4 Antibody (N-Terminus)  
LGR4 / GPR48 Rabbit Aff - Purified Bear, Bov, Can, Elephant, Hu, Mky, Ms, Por, Prim, Rb, Rt P
50 µg / €400.00
  OriGene Technologies, Inc.

LGR4 / GPR48 (C-term) antibody

  Rabbit Polyclonal Anti-LGR4 Antibody (C-Terminus)  
LGR4 / GPR48 Rabbit Aff - Purified Bear, Bov, Can, Elephant, Eq, Hst, Hu, Mky, Ms, Op, Platypus, Por, Prim, Rb, Rt, Tr, Xen P
50 µg / €400.00
  OriGene Technologies, Inc.

LGR4 / GPR48 antibody

  Rabbit Polyclonal Anti-LGR4 Antibody (Cytoplasmic Domain)  
Anti-LGR4 antibody IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu, Mky, Por, Rb
50 µg / €400.00
  OriGene Technologies, Inc.

LGR4 / GPR48 antibody

  Rabbit Polyclonal Anti-LGR4 Antibody (C-Terminus)  
Anti-LGR4 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Bov, Bt, Can, Chk, Eq, Hu, Ms, Por, Rt
50 µg / €400.00
  OriGene Technologies, Inc.

LGR4 / GPR48 antibody

  Rabbit Polyclonal Anti-LGR4 Antibody (N-Terminus)  
Anti-LGR4 antibody IHC of human brain, caudate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu
50 µg / €400.00
  OriGene Technologies, Inc.

LGR4 / GPR48 antibody

  Rabbit Polyclonal Anti-LGR4 Antibody  
LGR4 / GPR48 Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

LGR4 / GPR48 antibody

FACS analysis of BOSC23 cells using BBX-3A8 Cat.-No AM32985PU-N. BOSC23 cells were transiently transfected with an expression vector encoding either LGR4-hum (red curve), LGR4-murine (blue curve) or an irrelevant protein (control transfectant: black curve). Binding of BBX-3A8 was detected with a PE-conjugated secondary antibody. A positive signal was obtained with LGR4- hum and LGR4-mur transfected cells. Rat IgG2a BBX-3A8 Purified Hu, Ms E, F
0.1 mg / €360.00
  Acris Antibodies GmbH

LGR4 / GPR48 antibody

FACS analysis of BOSC23 cells using BBX-1H1 Cat.-No AP32978PU-N. BOSC23 cells were transiently transfected with an expression vector encoding either LGR4-hum (red curve), LGR4-murine (blue curve) or an irrelevant protein (control transfectant: black curve). Binding of BBX-3A8 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with LGR4-hum transfected cells. Rat IgG2a BBX-1H1 Purified Hu E, F
0.1 mg / €360.00
  Acris Antibodies GmbH

LGR4 / GPR48 antibody

Western blot analysis of extracts from Jurkat cells, using LGR4 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
50 µg / €230.00
  Assay Biotech

LGR4 / GPR48 antibody

Western blot analysis of extracts from Jurkat cells, using LGR4 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

LGR4 - human gene knockout kit via CRISPR

LGR4 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

LGR4 - human gene knockout kit via CRISPR

LGR4 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

LGR4 - human gene knockout kit via CRISPR

LGR4 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

LGR4 - human gene knockout kit via CRISPR

LGR4 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lgr4 - mouse gene knockout kit via CRISPR

Lgr4 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Lgr4 - mouse gene knockout kit via CRISPR

Lgr4 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

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