LIMA1 antibody

Principal name

LIMA1 antibody

Alternative names for LIMA1 antibody

LIMA1, EPLIN, SREBP3, LIM domain and actin-binding protein 1, Epithelial protein lost in neoplasm

SwissProt ID

Q9UHB6 (Human)

Gene ID

51474 (LIMA1)

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Immunoprecipitation (IP), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of LIMA1 antibody

Epithelial protein lost in neoplasm (EPLIN) is a cytoskeleton-associated protein characterized by the presence of a single centrally located lin-11, isl-1 and mec-3 (LIM) domain. It also contains at least two Actin-binding domains, in which the C-terminal domain binds more effectively than the N-terminal domain. By binding Actin monomers and filaments, EPLIN is involved in regulation of the Actin cytoskeleton by increasing the number and size of Actin stress fibers, delaying filament nucleation, reducing formation of branched filaments and bundling of Actin filaments. It also inhibits membrane ruffling and Actin filament depolymerization. EPLIN is strongly expressed in placenta, kidney, pancreas, prostate, ovary, spleen and heart, and to a lesser degree in lung, liver, brain, skeletal muscle, thymus, testis and intestine. It is expressed as two isoforms, EPLIN-α and EPLIN-β. Downregulation of EPLIN-α expression may contribute to the motility of invasive tumor cells.

General readings

Chircop, M., et al. Cell Cycle 8(5):757-764(2009)
Abe, K., et al. Proc. Natl. Acad. Sci. U.S.A. 105(1):13-19(2008)
Jiang, W.G., et al. Mol. Cancer 7, 71 (2008)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

LIMA1 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-LIMA1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808537)(1:150) Mouse IgG1 OTI1A3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

LIMA1 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-LIMA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808539)(1:150) Mouse IgG2a OTI6F7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

LIMA1 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-LIMA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808560)(1:150) Mouse IgG1 OTI9F3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

LIMA1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-LIMA1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808565)(1:150) Mouse IgG1 OTI1B4 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

LIMA1 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-LIMA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808587)(1:150) Mouse IgG1 OTI7H8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

LIMA1 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-LIMA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808601)(1:150) Mouse IgG1 OTI7H5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

LIMA1 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-LIMA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808615)(1:150) Mouse IgG1 OTI8F7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

LIMA1 antibody

WB Suggested Anti-EPLIN Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: Human Placenta Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

LIMA1 antibody

WB Suggested Anti-LIMA1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: Human Placenta Rabbit IgG Purified Eq, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

LIMA1 antibody

Western blot analysis of LIMA1 in human colon tissue lysate with LIMA1 antibody at (A) 0.5 and (B) 1 ug/mL. Rabbit IgG Purified Hu ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

LIMA1 (N-term) antibody

Western blot analysis of LIMA1 Antibody (N-term) Cat.-No AP52484PU-N in A549 cell line lysates (35ug/lane). This demonstrates the LIMA1 antibody detected the LIMA1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

LIMA1 antibody

LIMA1 Mouse IgG2a 1B7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

LIMA1 antibody

Human Placenta; WB Suggested Anti-EPLIN Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Human Placenta; LIMA1 Antibody - middle region (ARP34552_P050) in Human Placenta cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Por WB
50 µg / €350.00
  AVIVA Systems Biology

LIMA1 antibody

Hum. Fetal Heart; Host:Rabbit. Target Name:LIMA1. Sample Tissue:Human Fetal Heart. Antibody Dilution: 1.0ug/ml.; LIMA1 antibody - N-terminal region (ARP34817_P050) in Hum. Fetal Heart cells using Western Blot Rabbit Aff - Purified Hu, Ms WB
50 µg / €350.00
  AVIVA Systems Biology

LIMA1 antibody

Western Blot: EPLIN Antibody [NB100-2305] - Detection of Human EPLIN by Western Blot. Samples:  A) Whole cell lysate (50 mcg) from DU145 and SKBR3 cells. B) EPLIN that was immunoprecipitated from 500 mcg of whole cell lysate from DU145 cells.  Antibodies:  Affinity purified rabbit anti-EPLIN antibody NB100-2035 was used at 0.3 mcg/ml (A) or 1 mcg/ml (B) for WB. In B, EPLIN was immunoprecipitated using another rabbit anti-EPLIN antibody.  Detection: Chemiluminescence with exposure times of 30 minutes (A) or 30 seconds (B). Rabbit Aff - Purified Hu IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

LIMA1 antibody

Immunoprecipitation: EPLIN Antibody [NB100-2310] - Detection of Human EPLIN by Immunoprecipitation.  Samples:  Whole cell lysate (500 mcg) from DU145 cells.  Antibodies:  Affinity purified rabbit anti-EPLIN antibody NB100-2310 used at 10 mcg/mg lysate for IP.  Detection:  Chemiluminescence with an exposure time of 30 seconds. Rabbit Aff - Purified Hu IP
0.1 mg / €440.00
  Novus Biologicals Inc.

LIMA1 antibody

Immunohistochemistry-Paraffin: EPLIN Antibody [NBP1-87947] - Immunohistochemistry-Paraffin: EPLIN Antibody [NBP1-87947] Immunohistochemical staining of human stomach shows strong membranous and cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

LIMA1

LIMA1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

LIMA1

LIMA1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

LIMA1 overexpression lysate

LIMA1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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