LIME1 antibody

Principal name

LIME1 antibody

Alternative names for LIME1 antibody

LIME1, LIME, LIME-1, Lck-interacting membrane protein, Lck-interacting molecule, Lck-interacting transmembrane adapter 1

SwissProt ID

Q9EQR5 (Mouse), Q9H400 (Human)

Gene ID

54923 (LIME1), 72699 (Lime1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit, Goat

Available applications

Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), Enzyme Immunoassay (E), Flow Cytometry (F), ELISA (detection) (E(detection))

Background of LIME1 antibody

LIME (Lck-interacting molecule) is a 31 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.

General readings

1. Horejsi V, Zhang W, Schraven B.: Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat Rev Immunol. 2004 Aug;4(8):603-16.
2. Simeoni L, Smida M, Posevitz V, Schraven B, Lindquist JA.: Right time, right place: the organization of membrane proximal signaling. Semin Immunol. 2005 Feb;17(1):35-49.
3. Tedoldi S, Paterson JC, Hansmann ML, Natkunam Y, Rüdiger T, Angelisova P, Du MQ, Roberton H, Roncador G, Sanchez L, Pozzobon M, Masir N, Barry R, Pileri S, Mason DY, Marafioti T, Horejsi V.: Transmembrane adaptor molecules: a new category of lymphoid-cell markers. Blood. 2006 Jan 1;107(1):213-21.
4. Brdickova N, Brdicka T, Angelisová P, Horváth O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsi V. LIME: a new membrane Raft-associated adaptor
protein involved in CD4 and CD8 coreceptor signaling. J Exp Med. 2003 Nov 17;198(10):1453-62.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

LIME1 antibody

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-LIME1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA807703)(1:150) Mouse IgG1 OTI5D3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

LIME1 antibody

TA305824 staining (1ug/ml) of Mouse Heart lysate (RIPA buffer, 35ug total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

LIME1 antibody

Host: Rabbit; Target Name: LIME1; Sample Tissue: Fetal Lung lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

LIME1 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-LIME1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807653) Mouse IgG1 OTI3B10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

LIME1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIME1 (RC201909, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIME1.(1:2000) Mouse IgG1 OTI7A9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

LIME1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIME1 (RC201909, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIME1. Mouse IgG1 OTI9E9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

LIME1 (86-202) antibody

Western blot of  mouse thymocyte lysate (1% SDS); non-reduced sample, immunostained by mAb mLIME-05 and goat anti-mouse IgG (H+L)-HRP conjugate. Mouse IgG2a mLIME-05 Purified Ms IP, WB
0.1 mg / €260.00
  Acris Antibodies GmbH

LIME1 (141-295) antibody

Spleen, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Serum Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

LIME1 antibody

Western Blotting analysis (reducing conditions) of human LIME using anti-human LIME (LIME-10). Lane 1: J77 cell line transfected with LIME. Lane 2: non-transfected J77 cell line (cells are essentialy devoid of endogenous LIME expression). Mouse IgG2a LIME10 Purified Hu WB
0.1 mg / €260.00
  Acris Antibodies GmbH

LIME1 antibody

Induction of LIME tyrosine phosphorylation. Peripheral blood T cells were left unstimulated (-) or stimulated with anti-human CD3 (MEM-92; SM3152P)  or anti-human CD4 (MEM-16; AM03098PU-N), and LIME was immunoprecipitated from laurylmaltoside lysates with the LIME-06 antibody (immunoaffinity sorbent) and analyzed by Western blotting to visualize tyrosine-phosphorylated LIME (top) and total LIME (bottom). Mouse IgG1 LIME-06 Purified Hu IP
0.1 mg / €260.00
  Acris Antibodies GmbH

LIME1 antibody

LIME1 Mouse IgG1 LIME-06 PE Hu F
0.1 mg / €410.00
  Acris Antibodies GmbH

LIME1 antibody

Immunohistochemistry staining of human spleen (paraffin sections) with anti-LIME polyclonal antibody. Rabbit Purified Hu P, WB
0.1 mg / €260.00
  Acris Antibodies GmbH

LIME1 antibody

LIME1 Rabbit Purified Ms IP, WB
0.1 mg / €260.00
  Acris Antibodies GmbH

LIME1 antibody

Western Blot analysis of LIME1 expression in transfected 293T cell line (H00054923-T01) by LIME1 MaxPab polyclonal antibody.

Lane 1: LIME1 transfected lysate(32.45 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

LIME1 antibody

Induction of LIME1 tyrosine phosphorylation. Peripheral blood T cells were left unstimulated (-) or stimulated with anti-human CD3 ( MEM-92 ) or anti-human CD4 ( MEM-16 ), and LIME1 was immunoprecipitated from laurylmaltoside lysates with the LIME1 monoclonal antibody, clone LIME-06 ( Cat # MAB0960 ) ( immunoaffinity sorbent ) and analyzed by Western blotting to visualize tyrosine-phosphorylated LIME1 ( top ) and total LIME1 ( bottom ). Mouse IgG1 LIME-06 Hu IP
0.1 mg / €390.00
  Abnova Taiwan Corp.

LIME1 antibody

LIME1 Mouse IgG1 LIME-06 Hu F
0.1 mg / €470.00
  Abnova Taiwan Corp.

LIME1 antibody

The Lime1 polyclonal antibody ( Cat # PAB6643 ) staining ( 1 µg/ml ) of mouse heart lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Ms E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

LIME1 antibody

LIME1 Rabbit Ms IP, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

LIME1 antibody

NB 100-1436 staining (1µg/ml) of Mouse Heart lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat IgG Aff - Purified Ms E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

LIME1 antibody

Western Blot: LIME Antibody (LIME-10) [NB500-343] - Fig. 1. Western Blotting analysis (reducing conditions) of human LIME using anti-human LIME (LIME-10). Lane 1: J77 cell line transfected with LIME Lane 2: non-transfected J77 cell line (cells are essentialy devoid of endogenous LIME expression). Mouse IgG2a LIME-10 Purified Hu P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

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